Table 4Type II and IIS restriction enzymes isolated from various H. pylori strains

StrainRestriction enzymeRecognition sequenceaPrototypeOrigin (reference)b
26695HpyAIIGAAGAMboIIUK (30)
HpyAIIIGATCMboI
HpyAIVGANTCHinfI
HpyAVGACNNNNNGTCEam1105I
J99Hpy99I[down double arrow]CGWCGNoneUSA (3)
Hpy99IIGTSACTsp45I
Hpy99IIIGCGCHhaI
Hpy99IVCCNNGGSecI
60190HpyIIGAAGAMboIIUK (10)
HpyIVGANTCHinfI
HpyVTCGATaqI
HpyVIIICCGGHpaII
J178Hpy178IIGAAGAMboIIUSA (23)
Hpy178IIITC[down double arrow]NNGANone
Hpy178VIGGATGFokI
Hpy178VIIGGCCHaeIII
J188Hpy1881TCN[down double arrow]GANoneUSA (23)
Hpy188IIITCNNGAHpy178III
J166Hpy166ITCNGAHpy188IUSA (23)
Hpy166IIGTNNACMjaIV
Hpy166IIICCTCMnlI
CH4HpyCH4IC[down double arrow]ATGNlaIIIChina
HpyCH4IICTNAGDdeI
HpyCH4IIIACN[down double arrow]GTTsp4CI
HpyCH4IVA[down double arrow]CGTMaeII
HpyCH4VTG[down double arrow]CACviRI
HpyCH4VITCNNGAHpy178III
HPK5HpyHPK5ICTNAGDdeIJapan (31)
HpyHPK5IIGATCMboI
A8-5Hpy8IGTNNACMjaIVLithuania (25)
Hpy8IIGTSACTsp45I
Hpy8IIIGWGCWCHgiAI
JP26HpyJP26ITGCACviRIJapan
HpyJP26IITCGATaqI
RobertsHpyBIGT[down double arrow]ACRsaIUK
HpyBIIGTN[down double arrow]NACMjaIV
NCTC11637HpyCIGAT[down double arrow]ATCEcoRVAustralia (2)
RFL10HpyF10IGCGCHhaILithuania (25)
HpyF10IIGANTCHinfI
HpyF10IIICCNNGGSecI
HpyF10IVGTACRsaI
HpyF10VGGCCHaeIII
HpyF10VIGCNNNNNNNGCMwoI
RFL35HpyF35ITCGATaqILithuania (25)
HpyF35IIACGTMaeII
HpyF35IIIACNGTTsp4CI
HpyF35IVGTSACTsp45I
a

Cleavage sites, when known, are indicated by arrows. W, A or T; S, G or C; N, any nucleotide.

b

UK, United Kingdom; USA, United States.

From: Chapter 24, Restriction and Modification Systems

Cover of Helicobacter pylori
Helicobacter pylori: Physiology and Genetics.
Mobley HLT, Mendz GL, Hazell SL, editors.
Washington (DC): ASM Press; 2001.
Copyright © 2001, ASM Press.

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