Figure 19-35. Fluorescence micrographs showing the organization of chromosomes and microtubules during four mitotic stages.

Figure 19-35Fluorescence micrographs showing the organization of chromosomes and microtubules during four mitotic stages

Cultured PtK2 fibroblasts were stained with a fluorescent anti-tubulin antibody (green) and the DNA-binding dye ethidium homodimer (purple). Both photographs in each panel show the same cell, stained with both reagents (top) or just anti-tubulin antibody (bottom). Thus, in the top panels the areas of overlap are blue, and serve to highlight DNA. (a) During early prophase, the nucleus is surrounded by interphase microtubules. (b) By late prophase, the nuclear membrane has broken down and the chromosomes have condensed. The replicated centrosomes (centrioles) have migrated to the poles of the developing spindle. The microtubules radiate from the poles. (c) At metaphase, the chromosomes have aligned midway between the poles to form the metaphase plate. Dense bundles of microtubules connect the chromosomes to the poles. The purple stain of the chromosomes is visible outside the spindle, where there is no overlap with the green of the microtubules. (d) During late anaphase the chromosomes are pulled to the poles along the radiating microtubules. [From J. C. Waters, R. W. Cole, and C. L. Rieder, 1993, J. Cell Biol. 122:361; courtesy of C. L. Rieder.]

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From: Section 19.5, Microtubule Dynamics and Motor Proteins during Mitosis

Cover of Molecular Cell Biology
Molecular Cell Biology. 4th edition.
Lodish H, Berk A, Zipursky SL, et al.
New York: W. H. Freeman; 2000.
Copyright © 2000, W. H. Freeman and Company.

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