Figure 7.8. Exon trapping.

Figure 7.8Exon trapping

The exon-trap vector consists of two exon sequences preceded by promoter sequences - the signals required for gene expression in a eukaryotic host (Section 9.2.2). New DNA containing an unmapped exon is ligated into the vector and the recombinant molecule introduced into the host cell. The resulting RNA transcript is then examined by RT-PCR to identify the boundaries of the unmapped exon.

From: Chapter 7, Understanding a Genome Sequence

Cover of Genomes
Genomes. 2nd edition.
Brown TA.
Oxford: Wiley-Liss; 2002.
Copyright © 2002, Garland Science.

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