Table 4.4Sizes of human genomic libraries prepared in different types of cloning vector

Number of clones*
Type of vectorInsert size (kb)P = 95%P = 99%
λ replacement18532 500820 000
Cosmid, fosmid40240 000370 000
P112577 000118 000
BAC, PAC30032 00050 000
YAC60016 00024 500
Mega-YAC1400685010 500

Calculated from the equation:

Image ch4e1.jpg

where N is the number of clones required, P is the probability that any given segment of the genome is present in the library, a is the average size of the DNA fragments inserted into the vector, and b is the size of the genome.

From: Chapter 4, Studying DNA

Cover of Genomes
Genomes. 2nd edition.
Brown TA.
Oxford: Wiley-Liss; 2002.
Copyright © 2002, Garland Science.

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