Figure 5.30. A method for blocking repetitive DNA sequences in a hybridization probe.

Figure 5.30A method for blocking repetitive DNA sequences in a hybridization probe

In this example the probe molecule contains two genome-wide repeat sequences (shown in green). If these sequences are not blocked then the probe will hybridize non-specifically to any copies of these genome-wide repeats in the target DNA. To block the repeat sequences, the probe is prehybridized with a DNA fraction enriched for repetitive DNA.

From: Chapter 5, Mapping Genomes

Cover of Genomes
Genomes. 2nd edition.
Brown TA.
Oxford: Wiley-Liss; 2002.
Copyright © 2002, Garland Science.

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