FIGURE 3.12. Schematic of the detection configuration for two-photon excited fluorescence.

FIGURE 3.12

Schematic of the detection configuration for two-photon excited fluorescence. The excitation laser light is depicted as a solid line, while the fluorescent signals are shown as dashed and dotted lines. The collection system shown below the sample stage can be added for samples that are not too optically thick, such as brain slice.

From: Chapter 3, In Vivo Two-Photon Laser Scanning Microscopy with Concurrent Plasma-Mediated Ablation Principles and Hardware Realization

Cover of In Vivo Optical Imaging of Brain Function
In Vivo Optical Imaging of Brain Function. 2nd edition.
Frostig RD, editor.
Boca Raton (FL): CRC Press; 2009.
Copyright © 2009, Taylor & Francis Group, LLC.

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