FIGURE 6.1. RH1691 is a fast and linear voltage-sensitive dye.


RH1691 is a fast and linear voltage-sensitive dye. (A) Chemical structure of the voltage sensitive dye RH1691. (B) The VSD RH1691 is a fluorescent dye excited at ~630 nm and emitting fluorescence >665 nm. The dye is thought to insert primarily into the plasma membrane lipid bilayer and its electrochemical structure is modified depending upon the transmembrane electrical field. This change in structure alters both the absorption and emission properties of the dye. (C) Biophysical measurements of RH1691 fluorescence applied to voltage-clamped Xenopus oocytes. Changing membrane potential (Vm, grey trace) results in well-correlated fluorescence changes (ΔF/F0, black trace). (D) RH1691 fluorescence changes linearly as a function of membrane potential (Vm) in measurements carried out in Xenopus oocytes. (E) Higher time resolution view of RH1691 fluorescence changes (black data points, grey trace shows a sigmoidal fit to the fluorescence data) following voltage changes (solid black line) in Xenopus oocytes. RH1691 follows membrane potential changes with sub-millisecond resolution. (Panels C, D, and E are modified from Berger, T., Borgdorff, A.J., Crochet, S., Neubauer, F.B., Lefort, S., Fauvet, B., Ferezou, I., Carleton, A., Luscher, H.R., and Petersen, C.C.H. 2007. J. Neurophysiol. 97: 3751–3762. With permission.)

From: Chapter 6, Imaging the Brain in Action: Real-Time Voltage- Sensitive Dye Imaging of Sensorimotor Cortex of Awake Behaving Mice

Cover of In Vivo Optical Imaging of Brain Function
In Vivo Optical Imaging of Brain Function. 2nd edition.
Frostig RD, editor.
Boca Raton (FL): CRC Press; 2009.
Copyright © 2009, Taylor & Francis Group, LLC.

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