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Riddle DL, Blumenthal T, Meyer BJ, et al., editors. C. elegans II. 2nd edition. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 1997.

Cover of C. elegans II

C. elegans II. 2nd edition.

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Section VIFrequency of Operons and Trans -Splicing

Although it was originally estimated that the products of only 10−15% of C. elegans genes were subjected to trans-splicing (Bektesh et al. 1988), it is now clear that approximately 70% are trans-spliced (Zorio et al. 1994). The original estimate, based on a hybrid-arrest translation experiment with an SL1 antisense oligonucleotide, failed to take account of the fact that the TMG caps on the trans-spliced mRNAs inhibit translation in the rabbit reticulocyte system (Darzynkiewicz et al. 1988). This resulted in a large underestimation of the frequency of trans-splicing. Zorio et al. (1994) estimated the frequency of SL1 and SL2 trans-splicing by two different methods. First, the published literature was surveyed for genes for which the 5′ends had been determined. Of the 115 genes included in the survey, 57% were trans-spliced to SL1, 13% to SL2, and 30% were not trans-spliced. Second, 2 Mb of sequenced genomic DNA were surveyed for the presence of potential trans-splice sites upstream of predicted genes, and genes in clusters were tested for SL2 specificity to determine whether they were likely to be in operons. The results of this analysis are remarkably congruent with the survey of reported genes. The products of 57% of genes are estimated to be trans-spliced to SL1, 16% to SL2, and 27% are not trans-spliced. Taken together, these estimates indicate that the products of about 70% of C. elegans genes are trans-spliced and about 25% of C. elegans genes are transcribed in operons.

Copyright © 1997, Cold Spring Harbor Laboratory Press.
Bookshelf ID: NBK20176
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