FIGURE 47.2. Glycosidases used for structural analysis.

FIGURE 47.2

Glycosidases used for structural analysis. (Left) A biantennary N-glycan is shown with exoglycosidases that can be used to remove each monosaccharide sequentially. Exoglycosidases act only on terminal sugars. Also shown are endoglycosidases that remove the intact N-glycan. N-Glycanase cleaves the GlcNAc-Asn bond, releasing the N-glycan and converting asparagine into aspartate. Endoglycosidase F cleaves between the core N-acetylglucosamine residues and therefore leaves an N-acetylglucosamine attached to the protein. (Right) Endoglycosidase H (Endo H) cleaves between the core N-acetylglucosamine residues of oligomannose or hybrid N-glycans that have at least four mannose residues as shown. Endo H does not act on complex N-glycans.

Symbol Key: FIGURE 47.2. Glycosidases used for structural analysis.

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From: Chapter 47, Structural Analysis of Glycans

Cover of Essentials of Glycobiology
Essentials of Glycobiology. 2nd edition.
Varki A, Cummings RD, Esko JD, et al., editors.
Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2009.
Copyright © 2009, The Consortium of Glycobiology Editors, La Jolla, California.

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