Figure 12. Ca2+-imaging results showing [Ca2+]i increases in primary human epidermal melanocytes (HEM) Black bars, non-transfected cells.

Figure 12Ca2+-imaging results showing [Ca2+]i increases in primary human epidermal melanocytes (HEM) Black bars, non-transfected cells

Red bars, HEMs cotransfected with shRNA to TRPML1. Cells were treated with selected TRPML3 activating compounds from 5 different scaffolds (SF-11 (Probe 2), -21, -22(not cell permeable), -31 (Probe 1), -41, and -51) and singleton SN-2 at 30 μM (mean values ± SEM, n ≥ 3 independent experiments with 5–10 cells). An expression vector for YFP was cotransfected with the shRNA for identification of the transfected cells. See (1) for details.

From: Identification of Selective Agonists of the Transient Receptor Potential Channels 3 (TRPML3)

Cover of Probe Reports from the NIH Molecular Libraries Program
Probe Reports from the NIH Molecular Libraries Program [Internet].

NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.