Figure 1.. Canonical biogenesis of microRNA.

Figure 1.

Canonical biogenesis of microRNA. MicroRNAs are transcribed on long primary transcripts (pri-miRNA) then cleaved by Drosha to form a 60-80 nt pre-miRNA, which is exported to the cytoplasm by Exportin-5 and Ran-GTP. The pre-miRNA is cleaved by Dicer, which removes the hairpin loop, forming a duplex. Argonaute (Ago) proteins separate the duplex, and release the star (*) strand. The mature 19-25 nt miRNA and Ago form the core of the RNA-induced silencing complex (RISC), which is guided by the miRNA to complementary sequences in target mRNA (mRNA). The binding of RISC results in translational inhibition, destabilization, or degradation of the targeted mRNA.

From: MicroRNAs and the Progression of Colorectal Cancer

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