Table 7-1Analytical Methods for Determining RDX in Biological Materials

Sample matrixPreparation methodAnalytical
method
Sample
detection
limit
Percent
recovery
Reference
PlasmaExtract with methylene chloride and pentane; filter; concentrateHPLC/TEA100 ng/LNo dataFine et al. 1984
PlasmaAdd NaCl/acetic acid solution to sample; extract with toluene; add water; evaporate organic phase; combine aqueous phase with acetonitrile-containing internal standard; filterHPLC/UV146 µg/L87.7 (spike levels 0–2,000 ng/g; SD±19–188 ng; CV 7–19)U.S. Army 1981a
Serum and urineMix sample with internal standard; clean up on C18-bonded-phase extraction column, eluting with methanol; concentrateHPLC/UV100 µg/L90±2.0–101±1.1 (1–10 mg/L in serum); 98±1.6–101±1.3 (1–10 mg/L in urine)Turley and Brewster 1987
KidneyAdd NaCl/acetic acid solution to sample; extract with toluene; add water; evaporate organic phase; combine aqueous phase with acetonitrile-containing internal standard; filterHPLC/UV95 ng/g99.5 (spike levels 0–2,000 ng/g; SD±12–58; CV 2–11)U.S. Army 1981a
Muscle/fatHomogenize sample; extract with acetonitrile; concentrate; add internal standard and purified water; filterHPLC/UV62 ng/g102.9 (spike levels 0–2,000 ng/g; SD±2.2–86; CV 3.9–14)U.S. Army 1981a
LiverHomogenize sample; add NaCl/acetic acid solution; evaporate; redissolve in acetonitrile-containing internal standard; filterHPLC/UV150 ng/g87.7 (spike levels 0–1,000 ng/g; SD±18–69; CV 7–22)U.S. Army 1981a
Hand swabsWipe hand with swab soaked in acetone; squeeze out acetone and concentrateHPLC/TEA; HRGC/TEA10 pg/injNo dataFine et al. 1984
Hand swabsWipe hand with swab soaked in ether; extract with ether; centrifuge to remove debris; decant supernatant and evaporate; redissolve in pentane; clean up on Amberlite XAD-7 beads, eluting with ethyl acetate; evaporate; redissolve in pentane and repeat Amberlite XAD-7 clean-upGC/ECD50 ng/swab (1.7 ng/inj)47 (at 200 ng/swab)Douse 1982
TLC20 ng/swabNo data
Hand swabs, standardsWipe hand with dry swab; extract with methanol/potassium phosphate; directly inject standardsHPLC/PMDE8 pg/inj (standards)No dataLloyd 1983

CV = coefficient of variation; ECD = electron capture detection; GC = gas chromatography; HPLC = high-performance liquid chromatography; HRGC = high-resolution gas chromatography; inj = injection; PMDE = pendant mercury drop electrode; TEA = thermal energy analyzer; TLC = thin layer chromatography; UV = ultraviolet

From: 7, ANALYTICAL METHODS

Cover of Toxicological Profile for RDX
Toxicological Profile for RDX.
Abadin H, Smith C, Ingerman L, et al.

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