Table 6VEGF stimulation assay protocol

SequenceParameterValueDescription
1Cells400 μL400,000 cells/well
2Time24 hIncubate at 37 °C and 5% CO2
3Reagent1 μLCompounds in DMSO, relaxin as positive control
4Time2 hIncubate at 37 °C and 5% CO2
5DetectorVEGF mRNA expressionRoche LightCycler 480

From: Discovery, optimization, and biological activity of the first potent and selective small-molecule agonist series of human relaxin receptor 1 (RXFP1)

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