Table 3Stepwise Protocol Used for the Hela, HepG2 or AML12 cell 1536-Well Assay

StepValueDescription
14 μLHeLa, HepG2 or AML12 cells (700/well) 2% FBS OPTI-MEM-phenol red free
2Time37°C incubation O/N
323 nLControls: SB 408124-lipid over storage; Triacsin C – lipid under storage; 20 μM each
423 nL46 μM to 3 nM compound
51 μLOleic acid 400 μM in medium
6Time37 °C incubation O/N
74 μLDyes (Bodipy/Cell Tracker Red)
8Time37 °C incubation 2hrs
9Time30 min at r.t.
10DetectAcumen (488 nm ex; 500–530 em; 575–640 em)

From: Modulators of Lipid Storage

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Probe Reports from the NIH Molecular Libraries Program [Internet].

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