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Figure 8: The set of rules after initial sorting using the CellProfiler Analyst classifier tool

Figure 8: The set of rules after initial sorting using the CellProfiler Analyst classifier tool. In this example, only 2 rules were found out of the specified maximum of 5, both pertaining to the mean pH3 intensity of the nuclei channel, indicating that this feature was sufficient to achieve perfect classification on the training set.

From: Advanced Assay Development Guidelines for Image-Based High Content Screening and Analysis

Cover of Assay Guidance Manual
Assay Guidance Manual [Internet].
Sittampalam GS, Gal-Edd N, Arkin M, et al., editors.
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