Figure 26. The vitreal surface of retinal flat-mounts prepared after the retinas were detached for 3 days and reattached for 28 days and labeled with antibodies to vimentin (red) and GFAP (green).

Figure 26

The vitreal surface of retinal flat-mounts prepared after the retinas were detached for 3 days and reattached for 28 days and labeled with antibodies to vimentin (red) and GFAP (green). A, high magnification image of the retinal surface. The Muller cell endfeet are largely club shaped, and the red color indicates a predominance of vimentin. The vitreal edge of some of these endfeet show predominant GFAP (yellow/green color). B, an image showing the outgrowth of ribbon-like extensions of Muller cells' cytoplasm into the vitreous. Within the retina the endfeet are red, indicating a predominance of vimentin, whereas the endfeet outgrowths into the vitreous are green, indicating a shift to GFAP. C, a complex epiretinal "membrane" growing on the vitreal surface. These membranes consist mainly of Muller cell processes in which the "leading" edge of the membrane shows a predominance of GFAP (green). D, a higher magnification image showing the complex structure of the epiretinal membranes with their mixture of Muller cell endfeet and branched cells, presumed to be astrocytes based on their structure and labeling with the antibody to GFAP (arrowheads).

From: Cellular Remodeling in Mammalian Retina Induced by Retinal Detachment

Cover of Webvision
Webvision: The Organization of the Retina and Visual System [Internet].
Kolb H, Fernandez E, Nelson R, editors.
Salt Lake City (UT): University of Utah Health Sciences Center; 1995-.

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