Figure 2: Fluorescence-based Membrane Potential Assay Format for Detecting Potassium Channel Activators

Figure 2: Fluorescence-based Membrane Potential Assay Format for Detecting Potassium Channel Activators. Fluorescent dyes are used to measure cell depolarization following addition of high potassium concentration to the extracellular solution. Potassium induces only a minimal change in membrane potential in control (top panels), while a large depolarization and fluorescent signal are seen after preincubation with an agonist.

From: Ion Channel Screening

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Assay Guidance Manual [Internet].
Sittampalam GS, Gal-Edd N, Arkin M, et al., editors.
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