Figure 6: . Fluorescent proteins often have overlapping excitation and emission spectra.

Figure 6:

Fluorescent proteins often have overlapping excitation and emission spectra. eGFP and eYFP have substantial overlap, making clear separation of the signals difficult, even with the best filter choices.

From: Assay Development Guidelines for Image-Based High Content Screening, High Content Analysis and High Content Imaging

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Assay Guidance Manual [Internet].
Sittampalam GS, Coussens NP, Nelson H, et al., editors.
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