Format

Send to:

Choose Destination

Links from EST

UI-E-CQ0

Identifiers
BioSample: SAMN00170174; EST: LIBEST_010279
Organism
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
Attributes
development stageadult
tissueeye
lab hostDH10B (Life Technologies) (T1 phage resistant)
tissueoptic nerve
re_1EcoR I
v_typePlasmid
re_2Not I
vectorpT7T3-Pac (Pharmacia) with a modified polylinker
Description

UI-E-CQ0 is a cDNA library containing the following tissue(s): optic nerve. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CCATTAAGTG. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

Submission
Children's Memorial Research Center, Soares, MB; 2002-02-27
Accession:
SAMN00170174
ID:
170174

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk