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Accession: PRJNA152661 ID: 152661

Macaca Fascicularis Differential Gene Expression Following Stroke: Control versus Tat-NR2B9c Treated Cortex at 1 hour and 6 hours following middle cerebral aretry occlusion (MCAO)

Differential gene expression comparison between cortex of placebo and PSD-95 inhibitor (Tat-NR2B9c), treated cynomolgus macaques at 1 and 6 hours after middle cerebral artery occlusion. The purpose of this study was to identify changes in differential gene expression in the cortex of animals receiving strokes across the two time points and bewtwee the two treatment groups. Differential gene expression was defined as the ratio of fluroescene detected for expressed sequence tags in the left, unoperated cortex versus the contralateral, right, ischemic cortex at each time point. Two colour, Agilent, 4x44k whole genome macaca mulata microarrays with sample rna hybridized to Cy5 channel and universal macaca mulatta rna control on the Cy3 channel were obtained for each hemisphere. Comparisons between treatment groups at each time point and within in each treatment group across each time point were made to detect differential gene expression. Significant differential gene expression was defined as greater than 2-fold change and p<0.05 by T-test. Overall design: Three condition experiment comparing the effect of treatment with placebo versus Tat-NR2B9c administered 5 min after MCAO with tissue samples collected 1h and 6h after MCAO and a second comparison of gene expression within each treatment group between 1h and 6h: 3 biological replicates in each group
AccessionPRJNA152661; GEO: GSE35589
Data TypeTranscriptome or Gene expression
ScopeMultispecies
PublicationsCook DJ et al., "Treatment of stroke with a PSD-95 inhibitor in the gyrencephalic primate brain.", Nature, 2012 Feb 29;483(7388):213-7
SubmissionRegistration date: 8-Feb-2012
University of Toronto
RelevanceModel Organism
Project Data:
Resource NameNumber
of Links
Publications
PubMed1
Other datasets
GEO DataSets1
GEO Data Details
ParameterValue
Data volume, Spots1004424
Data volume, Processed Mbytes15
Data volume, Supplementary Mbytes335

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