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Accession: PRJNA124729 ID: 124729

Gene expression changes in the medial prefrontal cortex and nucleus accumbens following abstinence from cocaine self-administration (Norway rat)

See Genome Information for Rattus norvegicus
Many studies of cocaine-responsive gene expression have focused on changes occurring during cocaine exposure, but few studies have examined the persistence of these changes with cocaine abstinence. Persistent changes in gene expression, as well as alterations induced during abstinence may underlie long-lasting drug craving and relapse liability. Results: Whole-genome expression analysis was conducted on a rat cocaine binge-abstinence model that has previously been demonstrated to engender increased drug seeking and taking with abstinence. Gene expression changes in two mesolimbic terminal fields (mPFC and NAc) were identified in a comparison of cocaine-naïve rats with rats after 10 days of cocaine selfadministration followed by 1, 10, or 100 days of enforced abstinence (n=6-11 per group). A total of 1,461 genes in the mPFC and 414 genes in the NAc were altered between at least two time points (ANOVA, p<0.05; ±1.4 fold-change). These genes can be subdivided into: 1) changes with cocaine self-administration that do not persist into periods of abstinence, 2) changes with cocaine self-administration that persist with abstinence, 3) and those not changed with cocaine self-administration, but changed during enforced abstinence. qPCR analysis was conducted to confirm gene expression changes observed in the microarray analysis. Overall design: Gene expression changes in two mesolimbic terminal fields (mPFC and Nac) were identified in a comparison of cocaine-naϊve rats with rats after 10 days of cocaine self-administration followed by 1, 10 or 100 days of enforced abstinence. 5-6 replicates per condition.
Project Type:Transcriptome or Gene expression
Attributes:Scope: Multiisolate; Material: Transcriptome; Capture: Whole; Method type: Array
Relevance:Model Organism
Other Accessions:GEO:GSE20885
Project Data:
Resource NameNumber
of Links
Publications
PubMed1
PMC1
Other datasets
GEO DataSets1
GEO Data Details
ParameterValue
Data volume, Spots1117017
Data volume, Processed Mbytes16
Data volume, Supplementary Mbytes32
Publications:
  • Freeman WM et al., "Gene expression changes in the medial prefrontal cortex and nucleus accumbens following abstinence from cocaine self-administration.", BMC Neurosci, 2010 Feb 26;11:29
Submission:
Registration date: 16-Mar-2010
Genome Sciences Facility, Penn State College of Medicine

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