Mouse and Rat
Frequently Asked Questions
How can I create a Definition line for my sequence submission automatically from within Sequin?
An easy way is to use the Annotate>Generate Definition Line menu item. This option creates Definition lines based on the source and feature annotations you have made to the record, and conforms to GenBank style guidelines. An existing sequence title (i.e., DEFINITION line) can be edited by double-clicking on it and making changes in the editing window that pops up.
What is the new Entrez Genomes equivalent of the alphabetical list containing chromosome-specific markers that I used to see in the old Entrez Genomes views of human chromosomes?
Although the old alphabetical marker list is no longer available, any marker that used to be in an Entrez Genomes human chromosome view can now be found using the new STS searching page (www.ncbi.nlm.nih.gov/genome/sts/query.cgi?).
The STS search page includes all markers from dbSTS and all the human maps used in Entrez Genomes and GeneMap '99.
There does not seem to be a print function in Cn3D. How can I print Cn3D images?
You can print a Cn3D image by first exporting the image as a GIF file using Cn3Ds File/Export/GIF function. You can then print this GIF image using most image-viewing programs.
Where can I get a very basic summary of my GenBank data access options?
For a basic summary of data access routes, take a look at www.ncbi.nlm.nih.gov/Genbank/GenbankSearch.html.
Is NCBI News available in PDF format?
Yes, in addition to HTML access to NCBI News, you can print a PDF version of the current and back issues at www.ncbi.nlm.nih.gov/About/newsletter.html.
If I run a BLAST search against only the nr database, am I likely to miss anything important?
Yes. The BLAST htgs (High Throughput Genomic Sequence) database is excluded from nr and must be searched separately; the same is true of the BLAST EST and GSS databases. The Microbial Genomes: Finished and Unfinished link on the main BLAST page provides access to data on 68 finished and unfinished microbial genomes, which are also not contained in the nr database. Researchers interested in BLASTing against human contig data should access these data by using the Human Genome BLAST link from the main BLAST page, rather than searching nr.