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| VecScreen | BLAST | PubMed | Entrez | Nucleotide | Genome |
If you need help evaluating the significance of the results, please see Interpretation of VecScreen Results.
BLASTN 2.2.12 [Aug-07-2005]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman
(1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
RID: 1130968697-15533-63348408207.BLASTQ3
Database: UniVec
2113 sequences; 539,198 total letters
If you have any problems or questions with the results of this search
please refer to the BLAST FAQs
Query= gnl|VecScreen|Example Database sequence with vector contamination
(1057 letters)
Distribution of Vector Matches on the Query Sequence
| 1 | 264 | 528 | 792 | 1057 |
 |
 |
 |
 |
Strong Moderate 
Weak
Segment of suspect origin:
Segments matching vector:
Strong match: 1009-1057
Moderate match: 12-33
Weak match: 34
Suspect origin: 1-11
> gnl|uv|X52328.1:714-808 pBluescript II SK(+) vector DNA, phagemid
excised from lambda ZAPII
Length=95
Score = 84.4 bits (42), Expect = 7e-14
Identities = 48/49 (97%), Gaps = 1/49 (2%)
Strand=Plus/Plus
Query 1009 GCCGCCACCGCGGTGGAGCTCCCAGCTTTTGTTCCCTTTAGTGAGGGTT 1057
|||||||||||||||||||||| ||||||||||||||||||||||||||
Sbjct 27 GCCGCCACCGCGGTGGAGCTCC-AGCTTTTGTTCCCTTTAGTGAGGGTT 74
> gnl|uv|U02448.1:3446-3536 Cloning vector pMAMneo-LUC
Length=91
Score = 44.4 bits (22), Expect = 0.075
Identities = 22/22 (100%), Gaps = 0/22 (0%)
Strand=Plus/Plus
Query 12 GGGcccccccTCGAGGTCGACC 33
||||||||||||||||||||||
Sbjct 29 GGGCCCCCCCTCGAGGTCGACC 50
> gnl|uv|U13860.1:6124-7626-49 pMSG cloning vector
Length=1552
Score = 32.4 bits (16), Expect = 308
Identities = 16/16 (100%), Gaps = 0/16 (0%)
Strand=Plus/Minus
Query 19 cccTCGAGGTCGACCC 34
||||||||||||||||
Sbjct 1528 CCCTCGAGGTCGACCC 1513
Database:
Posted date: Dec 12, 2002 2:17 PM
Number of letters in database: 539,198
Number of sequences in database: 2,113
Lambda K H
1.39 0.747 1.38
Gapped
Lambda K H
1.39 0.747 1.38
Matrix: blastn matrix:1 -5
Gap Penalties: Existence: 3, Extension: 3
Number of Sequences: 2113
Number of Hits to DB: 4605
Number of extensions: 360
Number of successful extensions: 360
Number of sequences better than 700: 82
Number of HSP's better than 700 without gapping: 82
Number of HSP's gapped: 352
Number of HSP's successfully gapped: 88
Number of extra gapped extensions for HSPs above 700: 230
Length of query: 1057
Length of database: 539198
Length adjustment: 14
Effective length of query: 1043
Effective length of database: 509616
Effective search space: 531529488
Effective search space used: 1750000000000
A: 0
X1: 10 (20.0 bits)
X2: 15 (30.0 bits)
X3: 25 (50.0 bits)
S1: 10 (20.4 bits)
S2: 16 (32.4 bits)
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Revised: July 28, 2009.