Morton Fetal Cochlea

Library Description

Organism:	Homo sapiens
Developmental stage:	16-22 week fetus
Organ:	ear
Tissue:	cochlea
Vector:	pBluescript SK-
Vector type:	phagemid (ampicillin resistant)
Cloning site 1:	EcoRI
Cloning site 2:	XhoI
Lab host:	SOLR cells (kanamycin resistant)

Reference: Genomics 23, 42-50 (1994) Cloned unidirectionally. Primer: Oligo dT. Fetal cochlea, normal. 37% of inserts <0.5 kb, 56% 0.5-1.0 kb, 7% >1 kb. Uni-ZAP XR Vector. Library constructed by N. Robertson, C. Morton. ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

Source: Wilson RK, Washington University School of Medicine

Gene Content Analysis

12,665 ESTs from this library were grouped into 4,719 UniGene entries (putative genes) [UniGene build #221, 30-Jul-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
197	15555	Hs.489142	Collagen, type I, alpha 2.
161	12712	Hs.111779	Secreted protein, acidic, cysteine-rich (osteonectin).
143	11291	Hs.443625	Collagen, type III, alpha 1.
137	10817	Hs.669724	Transcribed locus, strongly similar to NP_036454.1 LSM5 homolog, U6 small nuclear RNA associated isoform a [Homo sapiens].
80	6317	Hs.628678	Vimentin.
71	5606	Hs.535192	Eukaryotic translation elongation factor 1 alpha 1.
55	4343	Hs.389378	MON2 homolog (S. cerevisiae).
52	4106	Hs.374596	Tumor protein, translationally-controlled 1.
50	3948	Hs.36053	Chromosome 5 open reading frame 13.
50	3948	Hs.172928	Collagen, type I, alpha 1.
45	3553	Hs.644125	Potassium channel tetramerisation domain containing 12.
44	3474	Hs.586423	Eukaryotic translation elongation factor 1 alpha 1.
44	3474	Hs.520640	Actin, beta.
38	3000	Hs.8102	Ribosomal protein S20.
36	2842	Hs.109439	Osteoglycin.
34	2685	Hs.514581	Actin, gamma 1.
34	2685	Hs.118076	Ribosomal protein S4, X-linked.
31	2448	Hs.519909	Myristoylated alanine-rich protein kinase C substrate.
31	2448	Hs.183684	Eukaryotic translation initiation factor 4 gamma, 2.
29	2290	Hs.681002	Collagen, type I, alpha 1.
29	2290	Hs.524910	Ferritin, heavy polypeptide 1.
29	2290	Hs.387804	Poly(A) binding protein, cytoplasmic 1.
29	2290	Hs.313	Secreted phosphoprotein 1.
28	2211	Hs.643683	Integral membrane protein 2B.
28	2211	Hs.419463	Ribosomal protein L23a.
27	2132	Hs.41119	Otoraplin.
26	2053	Hs.650585	Laminin, beta 1.
26	2053	Hs.546261	Heterogeneous nuclear ribonucleoprotein A1.
26	2053	Hs.410037	Connective tissue growth factor.
26	2053	Hs.1787	Proteolipid protein 1.
25	1974	Hs.518827	Cyclin I.
23	1816	Hs.712658	Transcribed locus.
23	1816	Hs.21016	Coagulation factor C homolog, cochlin (Limulus polyphemus).
22	1737	Hs.75360	Carboxypeptidase E.
22	1737	Hs.590892	Collagen, type IX, alpha 1.
22	1737	Hs.136348	Periostin, osteoblast specific factor.
21	1658	Hs.507916	TSC22 domain family, member 1.
20	1579	Hs.521461	Neurofilament, light polypeptide.
20	1579	Hs.468442	Calmodulin 2 (phosphorylase kinase, delta).
20	1579	Hs.429581	Reticulon 4.
20	1579	Hs.422181	S100 calcium binding protein B.
20	1579	Hs.23616	Lin-28 homolog B (C. elegans).
19	1500	Hs.644633	TIMP metallopeptidase inhibitor 3.
19	1500	Hs.527193	Ribosomal protein S23.
19	1500	Hs.520348	Ubiquitin C.
18	1421	Hs.501012	Adducin 3 (gamma).
18	1421	Hs.495710	Glycoprotein M6B.
18	1421	Hs.434980	Amyloid beta (A4) precursor protein.
18	1421	Hs.372031	Peripheral myelin protein 22.
18	1421	Hs.302145	Hemoglobin, gamma G.
18	1421	Hs.203717	Fibronectin 1.
18	1421	Hs.125898	GNAS complex locus.
17	1342	Hs.633514	TIMP metallopeptidase inhibitor 2.
17	1342	Hs.519445	Nuclear receptor subfamily 2, group F, member 1.
17	1342	Hs.265174	Ribosomal protein L32.
16	1263	Hs.82129	Carbonic anhydrase III, muscle specific.
16	1263	Hs.76224	EGF-containing fibulin-like extracellular matrix protein 1.
16	1263	Hs.636480	Tubulin, beta.
16	1263	Hs.528305	DEAD (Asp-Glu-Ala-Asp) box polypeptide 17.
16	1263	Hs.511817	Pleckstrin homology domain interacting protein.
16	1263	Hs.505735	Nascent polypeptide-associated complex alpha subunit.
16	1263	Hs.495755	Retinoblastoma binding protein 7.
16	1263	Hs.487774	Heterogeneous nuclear ribonucleoprotein A2/B1.
16	1263	Hs.437638	X-box binding protein 1.
16	1263	Hs.356572	Ribosomal protein S3A.
15	1184	Hs.713950	Zinc finger and BTB domain containing 44.
15	1184	Hs.652183	ADP-ribosylation factor 4.
15	1184	Hs.557550	Nucleophosmin (nucleolar phosphoprotein B23, numatrin).
15	1184	Hs.523446	Collagen, type XI, alpha 1.
15	1184	Hs.437594	Ribosomal protein, large, P2.
15	1184	Hs.433701	Ribosomal protein L37a.
15	1184	Hs.348883	Forkhead box C1.
14	1105	Hs.712994	Septin 2.
14	1105	Hs.571841	Ribosomal protein L7.
14	1105	Hs.503178	Spectrin, beta, non-erythrocytic 1.
14	1105	Hs.436687	SET nuclear oncogene.
14	1105	Hs.356331	Peptidylprolyl isomerase A (cyclophilin A).
14	1105	Hs.165859	Anthrax toxin receptor 1.
13	1026	Hs.708235	Transcribed locus.
13	1026	Hs.654841	Solute carrier family 35, member F1.
13	1026	Hs.459927	Prothymosin, alpha.
13	1026	Hs.426296	Chromosome 10 open reading frame 104.
13	1026	Hs.407580	Plakophilin 4.
13	1026	Hs.150580	Eukaryotic translation initiation factor 1.
13	1026	Hs.120633	CDNA FLJ33813 fis, clone CTONG2002744.
12	947	Hs.576320	Chromosome 4 open reading frame 34.
12	947	Hs.522584	Thymosin beta 4, X-linked.
12	947	Hs.520703	Transcribed locus, strongly similar to NP_989488.2 eukaryotic translation elongation factor 1 alpha 1 [Gallus gallus].
12	947	Hs.507798	Lipoma HMGIC fusion partner.
12	947	Hs.459940	Lipopolysaccharide-induced TNF factor.
12	947	Hs.433529	Ribosomal protein S11.
12	947	Hs.408182	Collagen, type II, alpha 1.
12	947	Hs.34114	ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide.
12	947	Hs.131704	Neuropilin 1.
11	869	Hs.715135	Transcribed locus.
11	869	Hs.707896	FBJ murine osteosarcoma viral oncogene homolog.
11	869	Hs.705679	Transcribed locus.
11	869	Hs.643544	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide.
11	869	Hs.596087	Transcribed locus.
11	869	Hs.561432	Cri-du-chat region mRNA, clone NIBB11.