Human Fetal Cochlea cDNA Library

Library Description

Organism:	Homo sapiens
Developmental stage:	Fetal
Organ:	ear
Tissue:	cochlea
Vector:	pCR2.1
Vector type:	Plasmid
Cloning site 1:	EcoRI
Cloning site 2:	EcoRI
Lab host:	DH5alpha

Human fetal cochlea cDNA library normalized to brain, testis, kidney, uterus, and fibroblast according to Clontech PCR-Select cDNA Subtraction method.

Source: Battey, J.F., National Institute on Deafness and Other Communication Disorders

Gene Content Analysis

3,396 ESTs from this library were grouped into 1,113 UniGene entries (putative genes) [UniGene build #221, 30-Jul-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
191	56243	Hs.535192	Eukaryotic translation elongation factor 1 alpha 1.
164	48292	Hs.669724	Transcribed locus, strongly similar to NP_036454.1 LSM5 homolog, U6 small nuclear RNA associated isoform a [Homo sapiens].
154	45347	Hs.21016	Coagulation factor C homolog, cochlin (Limulus polyphemus).
90	26502	Hs.590892	Collagen, type IX, alpha 1.
72	21201	Hs.408182	Collagen, type II, alpha 1.
69	20318	Hs.644125	Potassium channel tetramerisation domain containing 12.
63	18551	Hs.586423	Eukaryotic translation elongation factor 1 alpha 1.
63	18551	Hs.518726	Integrin-binding sialoprotein.
54	15901	Hs.713764	Transcribed locus, strongly similar to XP_777977.1 hypothetical protein CNBA4460 [Cryptococcus neoformans var. neoformans B-3501A].
53	15607	Hs.36053	Chromosome 5 open reading frame 13.
41	12073	Hs.714673	Transcribed locus.
37	10895	Hs.681002	Collagen, type I, alpha 1.
34	10012	Hs.111779	Secreted protein, acidic, cysteine-rich (osteonectin).
33	9717	Hs.418012	Collagen, type IX, alpha 2.
33	9717	Hs.172928	Collagen, type I, alpha 1.
33	9717	Hs.118076	Ribosomal protein S4, X-linked.
29	8539	Hs.443625	Collagen, type III, alpha 1.
25	7362	Hs.82129	Carbonic anhydrase III, muscle specific.
23	6773	Hs.489142	Collagen, type I, alpha 2.
22	6478	Hs.716639	Collagen, type IX, alpha 3.
21	6184	Hs.499839	Ribosomal protein L7a.
19	5595	Hs.371249	Pleiotrophin.
18	5300	Hs.119689	Glycoprotein hormones, alpha polypeptide.
16	4711	Hs.529631	Ribosomal protein L35a.
16	4711	Hs.381061	Ribosomal protein L19.
16	4711	Hs.109439	Osteoglycin.
15	4417	Hs.546261	Heterogeneous nuclear ribonucleoprotein A1.
15	4417	Hs.372031	Peripheral myelin protein 22.
14	4122	Hs.76884	Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein.
14	4122	Hs.650585	Laminin, beta 1.
14	4122	Hs.533977	Transcribed locus, strongly similar to NP_006463.3 thioredoxin interacting protein [Homo sapiens].
14	4122	Hs.523446	Collagen, type XI, alpha 1.
14	4122	Hs.505735	Nascent polypeptide-associated complex alpha subunit.
14	4122	Hs.41119	Otoraplin.
13	3828	Hs.551713	Myelin basic protein.
12	3534	Hs.503178	Spectrin, beta, non-erythrocytic 1.
12	3534	Hs.436542	Cysteine-rich secretory protein LCCL domain containing 1.
12	3534	Hs.369840	Nidogen 2 (osteonidogen).
11	3239	Hs.676750	Tubulin, alpha 1b.
11	3239	Hs.437594	Ribosomal protein, large, P2.
11	3239	Hs.370504	Ribosomal protein S15a.
11	3239	Hs.34114	ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide.
10	2945	Hs.533336	BMP and activin membrane-bound inhibitor homolog (Xenopus laevis).
10	2945	Hs.512676	Ribosomal protein S25.
10	2945	Hs.28491	Spermidine/spermine N1-acetyltransferase 1.
10	2945	Hs.235935	Nephroblastoma overexpressed gene.
10	2945	Hs.212838	Alpha-2-macroglobulin.
9	2650	Hs.655229	Growth hormone 1.
9	2650	Hs.571841	Ribosomal protein L7.
9	2650	Hs.469473	Ribosomal protein L31.
9	2650	Hs.446149	Lactate dehydrogenase B.
8	2356	Hs.546287	Ribosomal protein S7.
8	2356	Hs.546285	Ribosomal protein, large, P0.
8	2356	Hs.487774	Heterogeneous nuclear ribonucleoprotein A2/B1.
8	2356	Hs.433529	Ribosomal protein S11.
8	2356	Hs.408073	Ribosomal protein S6.
8	2356	Hs.381219	Ribosomal protein L15.
8	2356	Hs.265174	Ribosomal protein L32.
7	2061	Hs.75360	Carboxypeptidase E.
7	2061	Hs.697218	Transcribed locus.
7	2061	Hs.644633	TIMP metallopeptidase inhibitor 3.
7	2061	Hs.546283	Ribosomal protein L6.
7	2061	Hs.524910	Ferritin, heavy polypeptide 1.
7	2061	Hs.523185	Ribosomal protein L13a.
7	2061	Hs.514196	Ribosomal protein L27.
7	2061	Hs.43125	Chromosome 2 open reading frame 40.
7	2061	Hs.429	ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C3 (subunit 9).
7	2061	Hs.302145	Hemoglobin, gamma G.
7	2061	Hs.150580	Eukaryotic translation initiation factor 1.
6	1767	Hs.712539	Hemoglobin, gamma A.
6	1767	Hs.709788	Transcribed locus, strongly similar to NP_058082.2 ras homolog gene family, member A precursor [Mus musculus].
6	1767	Hs.697561	Ribosomal protein L27.
6	1767	Hs.653700	Zic family member 2 (odd-paired homolog, Drosophila).
6	1767	Hs.643683	Integral membrane protein 2B.
6	1767	Hs.626461	Transcribed locus, strongly similar to NP_002037.2 glyceraldehyde-3-phosphate dehydrogenase [Homo sapiens].
6	1767	Hs.5662	Guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1.
6	1767	Hs.519520	Transcribed locus, strongly similar to NP_001005528.1 ribosomal protein s25 [Rattus norvegicus].
6	1767	Hs.435680	Epiphycan.
6	1767	Hs.413812	Ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1).
6	1767	Hs.412370	Ribosomal protein L9.
6	1767	Hs.390171	Collagen, type XI, alpha 2.
6	1767	Hs.374596	Tumor protein, translationally-controlled 1.
6	1767	Hs.356624	Nidogen 1.
6	1767	Hs.2171	Growth differentiation factor 10.
6	1767	Hs.116471	Cadherin 11, type 2, OB-cadherin (osteoblast).
5	1472	Hs.709037	Transcribed locus, moderately similar to XP_904556.1 PREDICTED: similar to hCG2016250, isoform 3 [Mus musculus].
5	1472	Hs.705910	Transcribed locus, strongly similar to NP_061932.1 translocase of outer mitochondrial membrane 7 homolog [Homo sapiens].
5	1472	Hs.632918	Phosphoglycerate mutase 1 (brain).
5	1472	Hs.632891	Transcribed locus, strongly similar to NP_001002079.1 ribosomal protein S13 [Danio rerio].
5	1472	Hs.597493	Transcribed locus, strongly similar to NP_001988.1 ubiquitin-like protein fubi and ribosomal protein S30 precursor [Homo sapiens].
5	1472	Hs.568341	Transcribed locus, strongly similar to NP_037356.1 ribosomal protein S26 [Rattus norvegicus].
5	1472	Hs.533683	Fibroblast growth factor receptor 2.
5	1472	Hs.518921	Nephronectin.
5	1472	Hs.518525	Glutamate-ammonia ligase (glutamine synthetase).
5	1472	Hs.477921	WW domain containing transcription regulator 1.
5	1472	Hs.464422	Collectin sub-family member 12.
5	1472	Hs.433732	CDC-like kinase 1.
5	1472	Hs.357004	Olfactomedin-like 2A.
5	1472	Hs.348883	Forkhead box C1.
5	1472	Hs.298280	ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle.