XGC-neurula

Library Description

Organism:	Xenopus (Silurana) tropicalis
Developmental stage:	neurula
Vector:	pCS107
Cloning site 1:	EcoRI
Cloning site 2:	NotI
Lab host:	Escherichia coli DH10B

cDNA was oligo dT primed from 5ug of poly A+ RNA from neurula. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.

Source: Huckle E, Sanger Institute

Gene Content Analysis

60,699 ESTs from this library were grouped into 9,188 UniGene entries (putative genes) [UniGene build #52, 23-Feb-2013]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
921	15173	Str.26251	Cold inducible RNA binding protein.
837	13789	Str.45491	Transcribed locus, strongly similar to NP_001017795.1 eef1a1b gene product [Danio rerio].
502	8270	Str.47402	Eukaryotic translation elongation factor 1 alpha 1, oocyte form.
383	6310	Str.54481	Keratin 5, gene 7.
375	6178	Str.47047	High mobility group nucleosome binding domain 1.
266	4382	Str.3054	Heterogeneous nuclear ribonucleoprotein A1.
258	4250	Str.10351	Novel protein similar to prothymosin, alpha.
256	4218	Str.47152	Ribosomal protein S10.
255	4201	Str.46996	H2A histone family, member Z.
226	3723	Str.5144	High mobility group box 3.
223	3674	Str.8802	DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A.
217	3575	Str.4766	Adenine nucleotide translocase.
211	3476	Str.4859	Heterogeneous nuclear ribonucleoprotein K.
209	3443	Str.8244	Heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A).
209	3443	Str.64764	Transcribed locus, strongly similar to NP_001079367.1 nuclease-sensitive element-binding protein 1 [Xenopus laevis].
208	3427	Str.39900	High mobility group box 2.
187	3081	Str.6718	Protein arginine methyltransferase 1.
180	2965	Str.8025	Keratin 18.
175	2883	Str.47012	Heterogeneous nuclear ribonucleoprotein A2/B1.
157	2587	Str.5132	Keratin.
153	2521	Str.47687	Y box binding protein 1.
147	2422	Str.30228	Chromatin assembly factor 1 subunit A-B-like.
145	2389	Str.8693	Serine/arginine-rich splicing factor 2.
142	2339	Str.47109	KH domain containing, RNA binding, signal transduction associated 1.
138	2274	Str.6666	Actin, gamma 1.
138	2274	Str.64795	Tumor protein, translationally-controlled 1.
138	2274	Str.37180	Eukaryotic translation elongation factor 1 alpha 1.
136	2241	Str.18663	Glutamate-ammonia ligase.
132	2175	Str.64748	Nuclear casein kinase and cyclin-dependent kinase substrate 1.
131	2158	Str.5829	U2 small nuclear RNA auxiliary factor 2.
131	2158	Str.48006	Keratin family.
128	2109	Str.47669	Interleukin enhancer binding factor 3, 90kDa.
125	2059	Str.40699	High mobility group nucleosomal binding domain 2.
125	2059	Str.11677	Heterogeneous nuclear ribonucleoprotein A0.
120	1977	Str.45809	Heterogeneous nuclear ribonucleoprotein A/B.
117	1928	Str.56253	Prothymosin, alpha.
117	1928	Str.26145	Poly(rC) binding protein 3.
114	1878	Str.45700	Fused in sarcoma.
112	1845	Str.5346	ATPase, Na+/K+ transporting, alpha 1 polypeptide.
112	1845	Str.16534	DEAD (Asp-Glu-Ala-Asp) box helicase 5.
109	1796	Str.47629	Keratin 8.
106	1746	Str.7118	Ribosomal protein L8.
105	1730	Str.22949	NHP2 non-histone chromosome protein 2-like 1.
98	1615	Str.11570	Interleukin enhancer binding factor 2.
97	1598	Str.65067	Glycine amidinotransferase (L-arginine:glycine amidinotransferase).
97	1598	Str.48929	Aly/REF export factor.
97	1598	Str.40639	Nucleophosmin (nucleolar phosphoprotein B23, numatrin).
96	1582	Str.42992	MARCKS-like 1.
92	1516	Str.8768	Angiopoietin-related protein 1-like.
90	1483	Str.65076	Serine/arginine-rich splicing factor 1.
89	1466	Str.1816	ES1 protein homolog, mitochondrial-like.
88	1450	Str.51844	Cathepsin L2.
87	1433	Str.54401	Uncharacterized LOC100490255.
87	1433	Str.21783	Sec61 alpha 1 subunit.
86	1417	Str.44153	Heterogeneous nuclear ribonucleoprotein D-like.
85	1400	Str.8882	Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein.
84	1384	Str.20	LSM12 homolog.
83	1367	Str.47018	RAN, member RAS oncogene family.
82	1351	Str.11690	Ornithine decarboxylase 1.
81	1334	Str.6554	SET nuclear oncogene.
81	1334	Str.48144	Acidic (leucine-rich) nuclear phosphoprotein 32 family, member C.
81	1334	Str.42546	Chromobox homolog 3.
80	1318	Str.36225	Nucleolin.
78	1285	Str.64731	Transcribed locus, strongly similar to NP_001016659.1 LOC549413 gene product [Xenopus (Silurana) tropicalis].
77	1269	Str.66985	Testis expressed 9.
77	1269	Str.44888	Small nuclear ribonucleoprotein polypeptide C.
77	1269	Str.17058	Heterogeneous nuclear ribonucleoprotein H1 (H).
76	1252	Str.76042	Transcribed locus, strongly similar to NP_001006687.1 krt gene product [Xenopus (Silurana) tropicalis].
75	1236	Str.47505	Eukaryotic translation initiation factor 5A.
75	1236	Str.47413	Actin, beta.
74	1219	Str.6210	Heterogeneous nuclear ribonucleoprotein L.
73	1203	Str.8038	Ubiquitin C.
73	1203	Str.6550	Cytochrome c, somatic.
73	1203	Str.64737	Zinc finger protein 326.
73	1203	Str.3387	Thymopoietin.
73	1203	Str.27059	AHNAK nucleoprotein.
73	1203	Str.24811	Myelin expression factor 2.
73	1203	Str.24165	Uroplakin 1A.
72	1186	Str.65356	Heterogeneous nuclear ribonucleoprotein A3.
71	1170	Str.64962	Isocitrate dehydrogenase 2 (NADP+), mitochondrial.
71	1170	Str.46551	Solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3.
70	1153	Str.8015	Proline-rich nuclear receptor coactivator 2.
70	1153	Str.4482	Small nuclear ribonucleoprotein polypeptides B and B1.
70	1153	Str.32162	Eukaryotic translation elongation factor 2, gene 1.
70	1153	Str.27255	Serine/arginine-rich splicing factor 9.
69	1137	Str.8742	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide.
69	1137	Str.67023	RNA binding motif protein 6.
68	1120	Str.43172	GATA-binding factor 2-like.
67	1104	Str.7641	B-cell translocation gene 1, anti-proliferative.
67	1104	Str.65918	H3 histone, family 3A.
67	1104	Str.47462	Thyroid hormone receptor associated protein 3.
66	1087	Str.6453	Eukaryotic translation initiation factor 1B.
66	1087	Str.11075	Fibrillarin.
65	1071	Str.8020	Stem-loop binding protein.
65	1071	Str.2942	Eukaryotic translation initiation factor 4A1.
65	1071	Str.21525	Calnexin.
64	1054	Str.51066	Catenin (cadherin-associated protein), beta 1, 88kDa.
64	1054	Str.48519	Poly(A) binding protein, cytoplasmic 1.
63	1038	Str.27143	Hepatoma-derived growth factor.
62	1021	Str.7531	ADP-ribosylation factor 1.