NCI_CGAP_Pr22

Library Description

Organism:	Homo sapiens
Sex:	male
Organ:	prostate
Tissue:	normal prostate
Vector:	pT7T3D-PacI
Vector type:	phagemid (ampicillin resistant)
Lab host:	DH10B

1st strand cDNA was prepared from normal prostate bulk tissue, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized, and was constructed by Bento Soares and M. Fatima Bonaldo.

Source: Robert Strausberg, Ph.D.

Gene Content Analysis

7,602 ESTs from this library were grouped into 4,371 UniGene entries (putative genes) [UniGene build #236, 09-Mar-2013]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
64	8419	Hs.1968	Semenogelin I.
37	4867	Hs.469473	Ribosomal protein L31.
34	4473	Hs.8102	Ribosomal protein S20.
34	4473	Hs.255462	Microseminoprotein, beta-.
31	4078	Hs.171995	Kallikrein-related peptidase 3.
27	3552	Hs.410977	Transgelin.
25	3289	Hs.433701	Ribosomal protein L37a.
23	3026	Hs.433670	Ferritin, light polypeptide.
22	2894	Hs.460109	Myosin, heavy chain 11, smooth muscle.
22	2894	Hs.119598	Ribosomal protein L3.
21	2762	Hs.731513	Ribosomal protein L37.
21	2762	Hs.537218	Semenogelin II.
18	2368	Hs.5662	Guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1.
18	2368	Hs.535192	Eukaryotic translation elongation factor 1 alpha 1.
18	2368	Hs.523185	Ribosomal protein L13a.
17	2236	Hs.632717	Myosin, light chain 6, alkali, smooth muscle and non-muscle.
17	2236	Hs.504687	Myosin, light chain 9, regulatory.
17	2236	Hs.433427	Ribosomal protein S17-like.
16	2105	Hs.586423	Eukaryotic translation elongation factor 1 alpha 1.
16	2105	Hs.523463	Ribosomal protein L27a.
16	2105	Hs.408073	Ribosomal protein S6.
14	1842	Hs.645344	Ribosomal protein L13a pseudogene 5.
14	1842	Hs.534404	Ribosomal protein L10.
14	1842	Hs.534255	Beta-2-microglobulin.
14	1842	Hs.449909	Ribosomal protein SA.
14	1842	Hs.438429	Ribosomal protein S19.
14	1842	Hs.155597	Complement factor D (adipsin).
13	1710	Hs.66731	Homeobox B13.
13	1710	Hs.477028	Ribosomal protein L24.
13	1710	Hs.419463	Ribosomal protein L23a.
12	1579	Hs.515560	Kallikrein-related peptidase 2.
12	1579	Hs.280130	Ribosomal protein S24.
11	1447	Hs.75307	H1 histone family, member X.
11	1447	Hs.546285	Ribosomal protein, large, P0.
11	1447	Hs.516105	Actin, gamma 2, smooth muscle, enteric.
11	1447	Hs.387208	Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed.
10	1315	Hs.513463	Nuclear protein, transcriptional regulator, 1.
10	1315	Hs.500483	Actin, alpha 2, smooth muscle, aorta.
10	1315	Hs.433060	Acid phosphatase, prostate.
10	1315	Hs.195464	Filamin A, alpha.
10	1315	Hs.178551	Ribosomal protein L8.
9	1184	Hs.594952	Desmin.
9	1184	Hs.498569	Ribosomal protein S2.
9	1184	Hs.425125	Ribosomal protein L29.
9	1184	Hs.412370	Ribosomal protein L9.
9	1184	Hs.410817	Ribosomal protein L13.
9	1184	Hs.408054	Ribosomal protein L12.
9	1184	Hs.356572	Ribosomal protein S3A.
9	1184	Hs.148340	Ribosomal protein L10a.
8	1052	Hs.719951	Ribosomal protein L11.
8	1052	Hs.699841	Immunoglobulin heavy locus.
8	1052	Hs.652114	Ribosomal protein L28.
8	1052	Hs.632573	Transcribed locus, strongly similar to NP_001001593.1 rpl23a gene product [Danio rerio].
8	1052	Hs.546286	Ribosomal protein S3.
8	1052	Hs.529631	Ribosomal protein L35a.
8	1052	Hs.512676	Ribosomal protein S25.
8	1052	Hs.433529	Ribosomal protein S11.
8	1052	Hs.408236	Thioredoxin domain containing 17.
8	1052	Hs.374596	Tumor protein, translationally-controlled 1.
8	1052	Hs.356626	Chromosome 19 open reading frame 70.
8	1052	Hs.269577	Protein tyrosine phosphatase, receptor type, A.
8	1052	Hs.118076	Ribosomal protein S4, X-linked.
8	1052	Hs.111779	Secreted protein, acidic, cysteine-rich (osteonectin).
7	921	Hs.730597	Ribosomal protein L9.
7	921	Hs.663699	MICAL-like 2.
7	921	Hs.544577	Glyceraldehyde-3-phosphate dehydrogenase.
7	921	Hs.529517	Lactotransferrin.
7	921	Hs.502745	Fatty acid desaturase 2.
7	921	Hs.461787	Neudesin neurotrophic factor.
7	921	Hs.445351	Lectin, galactoside-binding, soluble, 1.
7	921	Hs.437594	Ribosomal protein, large, P2.
7	921	Hs.436657	Clusterin.
7	921	Hs.424126	Small EDRK-rich factor 2.
7	921	Hs.374650	Interferon induced transmembrane protein 3.
7	921	Hs.356502	Ribosomal protein, large, P1.
7	921	Hs.198269	NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3, 9kDa.
7	921	Hs.190518	MIS18 kinetochore protein homolog A (S. pombe).
7	921	Hs.146602	Ubiquinol-cytochrome c reductase, complex III subunit VII, 9.5kDa.
7	921	Hs.124299	Family with sequence similarity 167, member A.
6	789	Hs.696283	NOP16 nucleolar protein.
6	789	Hs.644033	Transcribed locus.
6	789	Hs.599039	Melanoma cell adhesion molecule.
6	789	Hs.54673	Tumor necrosis factor (ligand) superfamily, member 13.
6	789	Hs.524910	Ferritin, heavy polypeptide 1.
6	789	Hs.521212	Aldo-keto reductase family 1, member B1 (aldose reductase).
6	789	Hs.520973	Heat shock 27kDa protein 1.
6	789	Hs.502876	Ras homolog family member B.
6	789	Hs.485899	Ribosomal protein L41.
6	789	Hs.438227	Ribosomal protein L34.
6	789	Hs.435126	SLC2A4 regulator.
6	789	Hs.433951	Glutathione peroxidase 4.
6	789	Hs.433750	Eukaryotic translation initiation factor 4 gamma, 1.
6	789	Hs.432976	Nuclear receptor subfamily 1, group H, member 2.
6	789	Hs.407995	Macrophage migration inhibitory factor (glycosylation-inhibiting factor).
6	789	Hs.406683	Ribosomal protein S15.
6	789	Hs.381061	Ribosomal protein L19.
6	789	Hs.337251	Delta-like 2 homolog (Drosophila).
6	789	Hs.304682	Cystatin C.
6	789	Hs.275865	Transcribed locus, strongly similar to NP_035426.1 Rps18 gene product [Mus musculus].
6	789	Hs.234642	Aquaporin 3 (Gill blood group).