EN0092

Library Description

Organism:	Homo sapiens
Developmental stage:	Adult
Organ:	lung_normal
Vector:	puc18
Vector type:	Plasmid
Cloning site 1:	SmaI
Cloning site 2:	SmaI

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

309 ESTs from this library were grouped into 195 UniGene entries (putative genes) [UniGene build #236, 09-Mar-2013]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
13	42071	Hs.657308	Transcribed locus.
13	42071	Hs.531561	Epithelial membrane protein 2.
11	35599	Hs.118076	Ribosomal protein S4, X-linked.
9	29126	Hs.16426	Transcribed locus.
7	22654	Hs.522114	Clathrin, light chain A.
6	19417	Hs.464422	Collectin sub-family member 12.
5	16181	Hs.492859	UDP-N-acteylglucosamine pyrophosphorylase 1.
5	16181	Hs.475963	CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like.
4	12945	Hs.471381	Tensin 1.
4	12945	Hs.406799	RAB18, member RAS oncogene family.
4	12945	Hs.202351	Phosphatase domain containing, paladin 1.
3	9709	Hs.637850	Reticulon 4.
3	9709	Hs.62886	SPARC-like 1 (hevin).
3	9709	Hs.628096	3-hydroxy-3-methylglutaryl-CoA reductase.
3	9709	Hs.529959	FIG4 homolog, SAC1 lipid phosphatase domain containing (S. cerevisiae).
3	9709	Hs.524750	Nucleoporin 98kDa.
3	9709	Hs.479754	V-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog.
3	9709	Hs.446315	Family with sequence similarity 107, member B.
3	9709	Hs.429581	CDNA clone IMAGE:40108446.
3	9709	Hs.412370	Ribosomal protein L9.
3	9709	Hs.162757	Low density lipoprotein receptor-related protein 1.
2	6472	Hs.91531	Myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 6.
2	6472	Hs.744213	Podocalyxin-like.
2	6472	Hs.675535	Transcribed locus, strongly similar to NP_032436.1 Itm2b gene product [Mus musculus].
2	6472	Hs.640836	Chromosome 6 open reading frame 48.
2	6472	Hs.621695	Metastasis associated lung adenocarcinoma transcript 1 (non-protein coding).
2	6472	Hs.594454	Fms-related tyrosine kinase 1.
2	6472	Hs.580300	Glutamine--fructose-6-phosphate transaminase 1.
2	6472	Hs.555992	Hydroxysteroid dehydrogenase like 1.
2	6472	Hs.546298	SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae).
2	6472	Hs.530251	TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa.
2	6472	Hs.523789	Nuclear paraspeckle assembly transcript 1 (non-protein coding).
2	6472	Hs.521487	Transmembrane protein 66.
2	6472	Hs.513083	Ribosomal protein L9.
2	6472	Hs.502705	PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae).
2	6472	Hs.4859	Cyclin L1.
2	6472	Hs.481992	Complement component 6.
2	6472	Hs.476018	Catenin (cadherin-associated protein), beta 1, 88kDa.
2	6472	Hs.446336	Paxillin.
2	6472	Hs.444673	CLPTM1-like.
2	6472	Hs.426704	Uncharacterized LOC100507412.
2	6472	Hs.386939	Ubiquitin specific peptidase 7 (herpes virus-associated).
2	6472	Hs.334832	Cytochrome b5 reductase 1.
2	6472	Hs.20529	Tumor protein p63 regulated 1-like.
2	6472	Hs.150837	Thioredoxin domain containing 5 (endoplasmic reticulum).
1	3236	Hs.87752	Moesin.
1	3236	Hs.87159	Calcium binding protein 39-like.
1	3236	Hs.80305	Rho GTPase activating protein 19.
1	3236	Hs.78065	Complement component 7.
1	3236	Hs.76206	Cadherin 5, type 2 (vascular endothelium).
1	3236	Hs.76152	Aquaporin 1 (Colton blood group).
1	3236	Hs.75969	Proline-rich nuclear receptor coactivator 1.
1	3236	Hs.745014	Spectrin repeat containing, nuclear envelope 2.
1	3236	Hs.743990	Tight junction protein 1.
1	3236	Hs.743241	Heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa).
1	3236	Hs.7426	HAUS augmin-like complex, subunit 5.
1	3236	Hs.740578	Zinc finger protein 512B.
1	3236	Hs.731828	Transmembrane protein 198B, pseudogene.
1	3236	Hs.731443	Transcribed locus.
1	3236	Hs.727543	Heat shock 60kDa protein 1 (chaperonin).
1	3236	Hs.724231	Transcribed locus.
1	3236	Hs.720020	Transcribed locus.
1	3236	Hs.719980	ATP/GTP binding protein 1.
1	3236	Hs.714048	MRNA; cDNA DKFZp586L0921 (from clone DKFZp586L0921).
1	3236	Hs.713075	Transcribed locus, strongly similar to NP_001026768.1 PABPC1 gene product [Gallus gallus].
1	3236	Hs.703834	Transcribed locus, moderately similar to XP_002729784.1 PREDICTED: hypothetical protein [Rattus norvegicus].
1	3236	Hs.703832	Transcribed locus, moderately similar to XP_002729393.1 PREDICTED: rCG64263-like [Rattus norvegicus].
1	3236	Hs.696631	Dab, mitogen-responsive phosphoprotein, homolog 2 (Drosophila).
1	3236	Hs.676114	CDNA FLJ31486 fis, clone NT2NE2001723.
1	3236	Hs.662187	CDNA FLJ36614 fis, clone TRACH2016380.
1	3236	Hs.661805	CDNA FLJ27080 fis, clone SPL03052.
1	3236	Hs.661625	CDNA FLJ32307 fis, clone PROST2002842.
1	3236	Hs.659231	WDR86 antisense RNA 1.
1	3236	Hs.657519	Uncharacterized LOC100294362.
1	3236	Hs.656673	Eukaryotic translation initiation factor 2 alpha kinase 4.
1	3236	Hs.655185	Transcribed locus.
1	3236	Hs.654572	Laminin, alpha 4.
1	3236	Hs.653654	Mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase.
1	3236	Hs.643702	Myotubularin related protein 6.
1	3236	Hs.643472	Heterogeneous nuclear ribonucleoprotein H3 (2H9).
1	3236	Hs.643447	Intercellular adhesion molecule 1.
1	3236	Hs.636983	CDNA FLJ46409 fis, clone THYMU3011081.
1	3236	Hs.631593	Protein phosphatase 1, regulatory subunit 15A.
1	3236	Hs.609074	Transcribed locus.
1	3236	Hs.60386	Nuclear RNA export factor 3.
1	3236	Hs.596913	Hydroxyprostaglandin dehydrogenase 15-(NAD).
1	3236	Hs.596014	Importin 9.
1	3236	Hs.594708	Neuralized homolog (Drosophila).
1	3236	Hs.594444	Lamin A/C.
1	3236	Hs.591908	A kinase (PRKA) anchor protein 2.
1	3236	Hs.587979	Prostate tumor overexpressed 1.
1	3236	Hs.567261	MAP/microtubule affinity-regulating kinase 2.
1	3236	Hs.558726	Pyruvate dehydrogenase phosphatase regulatory subunit pseudogene.
1	3236	Hs.546289	Ribosomal protein S12.
1	3236	Hs.535464	Eukaryotic translation initiation factor 3, subunit C-like.
1	3236	Hs.533468	HAUS augmin-like complex, subunit 6.
1	3236	Hs.533444	3-hydroxymethyl-3-methylglutaryl-CoA lyase.
1	3236	Hs.532793	Karyopherin (importin) beta 1.
1	3236	Hs.532363	Homeodomain interacting protein kinase 1.
1	3236	Hs.525527	RER1 retention in endoplasmic reticulum 1 homolog (S. cerevisiae).