Stratagene mouse heart (#937316)

Library Description

Organism:	Mus musculus
Strain:	NIH Swiss
Developmental stage:	13 day embryos
Sex:	pooled
Organ:	heart
Tissue:	heart
Vector:	pBluescript SK-
Vector type:	phagemid (ampicillin resistant)
Cloning site 1:	EcoRI
Cloning site 2:	XhoI
Lab host:	SOLR (kanamycin resistant)

Cloned unidirectionally. Primer: Oligo dT. 93 pooled NIH/Swiss 13 day embryo hearts. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

Source: Marra M/Mouse EST Project, Washington University School of MedicineP

Gene Content Analysis

8,237 ESTs from this library were grouped into 3,368 UniGene entries (putative genes) [UniGene build #194, 25-Jan-2013]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
147	17846	Mm.686	Actin, alpha, cardiac muscle 1.
130	15782	Mm.459653	Hemoglobin alpha, adult chain 1.
109	13233	Mm.440164	Hemoglobin Y, beta-like embryonic chain.
84	10198	Mm.441437	CDNA clone IMAGE:40049146.
49	5949	Mm.290003	Myosin, heavy polypeptide 6, cardiac muscle, alpha.
49	5949	Mm.288567	Hemoglobin, beta adult minor chain.
45	5463	Mm.455357	CDNA clone IMAGE:3587527.
44	5342	Mm.457983	Myosin, heavy polypeptide 7, cardiac muscle, beta.
43	5220	Mm.141758	Hemoglobin X, alpha-like embryonic chain in Hba complex.
41	4978	Mm.3862	Insulin-like growth factor 2.
37	4492	Mm.458364	Transcribed locus, strongly similar to NP_904330.1 cytochrome c oxidase subunit I [Mus musculus].
37	4492	Mm.343110	Glyceraldehyde-3-phosphate dehydrogenase.
30	3642	Mm.1529	Myosin, light polypeptide 2, regulatory, cardiac, slow.
29	3521	Mm.275831	Aldolase A, fructose-bisphosphate.
28	3399	Mm.44379	Troponin I, skeletal, slow 1.
27	3278	Mm.7353	Myosin, light polypeptide 3.
27	3278	Mm.273538	Tubulin, beta 5 class I.
26	3156	Mm.390355	Myosin, light polypeptide 4.
26	3156	Mm.304088	Glyceraldehyde-3-phosphate dehydrogenase.
26	3156	Mm.291442	Secreted acidic cysteine rich glycoprotein.
26	3156	Mm.276137	ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1.
26	3156	Mm.138471	Eukaryotic translation elongation factor 1 alpha 1.
25	3035	Mm.10728	Myosin binding protein C, cardiac.
24	2914	Mm.467412	Hemoglobin, beta adult major chain.
24	2914	Mm.391967	Actin, beta.
24	2914	Mm.121878	Tropomyosin 1, alpha.
22	2671	Mm.335639	Mesoderm specific transcript.
22	2671	Mm.193099	Fibronectin 1.
22	2671	Mm.1776	Ferritin heavy chain 1.
21	2549	Mm.238973	ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit.
20	2428	Mm.371545	Ribosomal protein, large, P0.
19	2307	Mm.6839	Cyclin-dependent kinase 4.
19	2307	Mm.458371	Transcribed locus, strongly similar to NP_904330.1 cytochrome c oxidase subunit I [Mus musculus].
19	2307	Mm.4222	Triosephosphate isomerase 1.
19	2307	Mm.16228	Solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 4.
18	2185	Mm.466752	CDNA clone IMAGE:4910858.
18	2185	Mm.4071	Ribosomal protein SA.
18	2185	Mm.392113	Tubulin, alpha 1B.
18	2185	Mm.247470	Troponin T2, cardiac.
18	2185	Mm.200916	Glutathione peroxidase 3.
17	2064	Mm.458386	Transcribed locus, strongly similar to NP_001101855.1 dematin [Rattus norvegicus].
17	2064	Mm.445729	Transcribed locus, strongly similar to NP_904337.1 NADH dehydrogenase subunit 4 [Mus musculus].
17	2064	Mm.20271	Fibrillin 2.
16	1942	Mm.6375	Glycogenin.
16	1942	Mm.326799	Eukaryotic translation elongation factor 2.
16	1942	Mm.271770	Myosin, light polypeptide 9, regulatory.
16	1942	Mm.268000	Vimentin.
16	1942	Mm.213025	Actin, alpha 2, smooth muscle, aorta.
15	1821	Mm.5291	Ribosomal protein S5.
15	1821	Mm.5286	Ribosomal protein, large, P0.
15	1821	Mm.373672	Titin.
15	1821	Mm.29324	Lactate dehydrogenase A.
14	1700	Mm.66	Ribosomal protein S4, X-linked.
14	1700	Mm.5305	Guanine nucleotide binding protein (G protein), beta polypeptide 2 like 1.
14	1700	Mm.46514	Myosin, light polypeptide 7, regulatory.
14	1700	Mm.455354	Transcribed locus, strongly similar to NP_036788.1 Sparc gene product [Rattus norvegicus].
14	1700	Mm.297482	Tumor protein, translationally-controlled 1.
14	1700	Mm.2180	Heat shock protein 90 alpha (cytosolic), class B member 1.
14	1700	Mm.154581	Aconitase 2, mitochondrial.
13	1578	Mm.30357	Ferritin light chain 1.
13	1578	Mm.196208	Regulator of G-protein signaling 12.
13	1578	Mm.14802	H19 fetal liver mRNA.
13	1578	Mm.125770	GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus.
12	1457	Mm.405761	Insulin-like growth factor binding protein 5.
12	1457	Mm.398119	Ornithine decarboxylase antizyme 1.
12	1457	Mm.348374	Transcribed locus, strongly similar to XP_002726683.1 PREDICTED: ferritin light chain 1-like [Rattus norvegicus].
12	1457	Mm.347009	Peroxiredoxin 2.
12	1457	Mm.280083	Ribosomal protein L4.
11	1335	Mm.738	Collagen, type IV, alpha 1.
11	1335	Mm.491107	Ribosomal protein L5.
11	1335	Mm.425090	Ribosomal protein S6.
11	1335	Mm.391708	Transcribed locus, strongly similar to NP_058834.1 Rpsa gene product [Rattus norvegicus].
11	1335	Mm.335460	Ubiquinol-cytochrome c reductase core protein 1.
11	1335	Mm.280103	ATPase, Na+/K+ transporting, alpha 1 polypeptide.
11	1335	Mm.234274	SET and MYND domain containing 1.
11	1335	Mm.12677	ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1.
10	1214	Mm.371570	Poly(A) binding protein, cytoplasmic 1.
10	1214	Mm.319347	NYN domain and retroviral integrase containing.
10	1214	Mm.297096	Malate dehydrogenase 2, NAD (mitochondrial).
10	1214	Mm.278560	ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit.
10	1214	Mm.256034	Chaperonin containing Tcp1, subunit 3 (gamma).
10	1214	Mm.227583	ATPase, Ca++ transporting, cardiac muscle, slow twitch 2.
10	1214	Mm.1970	Protein phosphatase 1, catalytic subunit, alpha isoform.
10	1214	Mm.196173	Transcribed locus, strongly similar to NP_033739.1 actin, cytoplasmic 2 [Mus musculus].
9	1093	Mm.9745	Lactate dehydrogenase B.
9	1093	Mm.726	Basigin.
9	1093	Mm.6712	Desmin.
9	1093	Mm.412745	Transcribed locus, strongly similar to NP_990334.1 HSPA8 gene product [Gallus gallus].
9	1093	Mm.399829	Ribosomal protein S3A1.
9	1093	Mm.39046	Filamin C, gamma.
9	1093	Mm.384744	Transcribed locus, strongly similar to NP_904329.1 NADH dehydrogenase subunit 2 [Mus musculus].
9	1093	Mm.265834	Solute carrier family 8 (sodium/calcium exchanger), member 1.
9	1093	Mm.246432	Isocitrate dehydrogenase 2 (NADP+), mitochondrial.
9	1093	Mm.133825	Cysteine rich protein 2.
8	971	Mm.43778	Ribosomal protein S14.
8	971	Mm.427589	Cell cycle associated protein 1.
8	971	Mm.379004	Ribosomal protein L7.
8	971	Mm.35811	Sarcalumenin.
8	971	Mm.282093	Ubiquitin pseudogene.
8	971	Mm.27674	Troponin I, cardiac 3.