NIH_XGC_tropTad5

Library Description

Organism:	Xenopus (Silurana) tropicalis
Developmental stage:	Tadpole (st. 36-41)
Tissue:	whole embryo
Vector:	pCS108
Cloning site 1:	SalI
Cloning site 2:	NotI
Lab host:	E. coli XL1-Blue derivative, Stratagene ElectroTen-Blue

Tadpole library constructed by Russell B. Fletcher in R. Harland's lab using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). SalI (5' end) -NotI (3' end) cDNA was inserted into vector pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html).

Source: Lindquist,E.A., Richardson,P., DOE Joint Genome Institute

Gene Content Analysis

105,277 ESTs from this library were grouped into 13,807 UniGene entries (putative genes) [UniGene build #52, 23-Feb-2013]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
914	8682	Str.41241	DnaJ (Hsp40) homolog, subfamily C, member 28.
862	8188	Str.26251	Cold inducible RNA binding protein.
781	7419	Str.45491	Transcribed locus, strongly similar to NP_001017795.1 eef1a1b gene product [Danio rerio].
500	4749	Str.37180	Eukaryotic translation elongation factor 1 alpha 1.
435	4132	Str.643	Ribosomal protein L3.
413	3923	Str.32162	Eukaryotic translation elongation factor 2, gene 1.
409	3885	Str.6666	Actin, gamma 1.
377	3581	Str.64787	Ribosomal protein S2.
331	3144	Str.6703	Ribosomal protein SA.
321	3049	Str.31592	Transcribed locus.
312	2964	Str.64764	Transcribed locus, strongly similar to NP_001079367.1 nuclease-sensitive element-binding protein 1 [Xenopus laevis].
312	2964	Str.47047	High mobility group nucleosome binding domain 1.
308	2926	Str.51958	Heat shock cognate 71 kDa protein-like.
307	2916	Str.65307	Myosin light chain, phosphorylatable, fast skeletal muscle.
302	2869	Str.68690	Pleckstrin homology domain containing, family B (evectins) member 2.
299	2840	Str.5646	Ribosomal protein, large, P0.
298	2831	Str.54481	Keratin 5, gene 7.
291	2764	Str.67961	Creatine kinase, muscle.
272	2584	Str.66708	Myosin, light chain 1, alkali; skeletal, fast.
272	2584	Str.64795	Tumor protein, translationally-controlled 1.
270	2565	Str.41473	Retinol binding protein 4, plasma.
259	2460	Str.17081	Myosin, heavy chain 8, skeletal muscle, perinatal.
248	2356	Str.1774	Ribosomal protein L6.
247	2346	Str.9053	Apolipoprotein A-I.
235	2232	Str.6701	Guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1.
233	2213	Str.48804	Parvalbumin, gene 2.
233	2213	Str.10351	Novel protein similar to prothymosin, alpha.
231	2194	Str.52164	Ribosomal protein L7.
225	2137	Str.51863	Uncharacterized LOC100170419.
220	2090	Str.41589	Peptidylprolyl isomerase A (cyclophilin A).
218	2071	Str.4766	Adenine nucleotide translocase.
217	2061	Str.25131	Cofilin 1 (non-muscle).
215	2042	Str.56253	Prothymosin, alpha.
213	2023	Str.47687	Y box binding protein 1.
210	1995	Str.65191	Creatine kinase, brain.
210	1995	Str.52469	Ribosomal protein S3A.
209	1985	Str.62377	Ribosomal protein L9.
209	1985	Str.26758	SERPINE1 mRNA binding protein 1.
207	1966	Str.65406	Tubulin, beta class I.
204	1938	Str.47464	Eukaryotic translation elongation factor 1 gamma.
202	1919	Str.29031	Oncomodulin.
199	1890	Str.68501	Parvalbumin.
190	1805	Str.20553	Ribosomal protein L5.
189	1795	Str.68447	Transcribed locus, strongly similar to XP_002935249.1 PREDICTED: myosin-4 [Xenopus (Silurana) tropicalis].
189	1795	Str.66716	ATPase, Ca++ transporting, cardiac muscle, fast twitch 1.
189	1795	Str.65118	Ferritin, heavy polypeptide 1.
189	1795	Str.34175	Heat shock protein 90kDa alpha (cytosolic), class B member 1.
187	1776	Str.47263	Ribosomal protein S5.
182	1729	Str.16974	Alpha globin larval-4.
176	1672	Str.6969	Ribosomal protein S6.
176	1672	Str.65140	Ribosomal protein L10a.
169	1605	Str.5620	Troponin T type 3 (skeletal, fast).
168	1596	Str.65949	Uncharacterized LOC496414.
167	1586	Str.84743	Ribosomal protein L10.
167	1586	Str.65146	Ribosomal protein S4, X-linked.
165	1567	Str.42135	Serine/arginine repetitive matrix 1.
162	1539	Str.5739	Ribosomal protein S3.
161	1529	Str.66747	Actin, alpha, cardiac muscle 1.
156	1482	Str.47413	Actin, beta.
152	1444	Str.66310	Ribosomal protein S9.
152	1444	Str.15334	Apolipoprotein B-100-like.
148	1406	Str.71268	Hemoglobin alpha 3 subunit.
148	1406	Str.5631	Troponin C type 2 (fast).
144	1368	Str.41468	Troponin I type 2 (skeletal, fast).
142	1349	Str.47505	Eukaryotic translation initiation factor 5A.
141	1339	Str.68555	Oncomodulin.
139	1320	Str.7118	Ribosomal protein L8.
139	1320	Str.40721	Ribosomal protein S8.
136	1292	Str.48519	Poly(A) binding protein, cytoplasmic 1.
135	1282	Str.66392	Ribosomal protein S12.
135	1282	Str.538	Calreticulin.
132	1254	Str.33529	Ribosomal protein L18a.
131	1244	Str.64876	Gastrula zinc finger protein XlCGF57.1-like.
130	1235	Str.55327	Tubulin, alpha 1b.
129	1225	Str.8252	Ribosomal protein L7a.
129	1225	Str.5144	High mobility group box 3.
128	1216	Str.36098	Ribosomal protein L17.
128	1216	Str.20	LSM12 homolog.
127	1206	Str.46551	Solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3.
127	1206	Str.39900	High mobility group box 2.
123	1168	Str.6170	Secreted protein, acidic, cysteine-rich (osteonectin).
122	1159	Str.47654	Ribosomal protein L15.
121	1149	Str.54515	Collagen, type II, alpha 1.
121	1149	Str.45809	Heterogeneous nuclear ribonucleoprotein A/B.
121	1149	Str.20174	Ribosomal protein L13.
118	1121	Str.36828	Ribosomal protein L11.
114	1083	Str.10372	Calmodulin 2 (phosphorylase kinase, delta).
112	1064	Str.48929	Aly/REF export factor.
112	1064	Str.4859	Heterogeneous nuclear ribonucleoprotein K.
109	1035	Str.41716	Tropomyosin 1 (alpha).
108	1026	Str.66727	Transcribed locus, strongly similar to XP_003125076.3 PREDICTED: actin, gamma-enteric smooth muscle-like, partial [Sus scrofa].
108	1026	Str.6147	Cell division cycle 42.
107	1016	Str.6694	Parvalbumin.
107	1016	Str.3054	Heterogeneous nuclear ribonucleoprotein A1.
101	959	Str.5791	Rhodopsin.
99	940	Str.9315	Alpha globin larval-5.
98	931	Str.18663	Glutamate-ammonia ligase.
98	931	Str.17066	Hemoglobin, delta.
97	921	Str.46639	Apolipoprotein E.
96	912	Str.65589	Ribosomal protein L18.