Full Length cDNA from the Mammalian Gene Collection

Library Description

Organism:	Homo sapiens
Tissue:	mixed
Vector:	mixed
Vector type:	Plasmid

The ORFs were PCR amplified from the MGC (Mammalian Gene Collection) and cloned by recombinational Gateway cloning into pDONR223 Donor vector. Reference : MGC (Mammalian Gene Collection) Program Team, Generation and Initial Analysis of more than 15,000 Full-Length Human and Mouse cDNA Sequences. PNAS, 2002, 99(26), 16899-16903

Source: Vidal M, Dana Farber Cancer Institute

Gene Content Analysis

11,407 ESTs from this library were grouped into 9,766 UniGene entries (putative genes) [UniGene build #236, 09-Mar-2013]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
14	1227	Hs.510635	Immunoglobulin heavy constant gamma 1 (G1m marker).
9	789	Hs.449609	Immunoglobulin kappa constant.
8	701	Hs.449585	Immunoglobulin lambda constant 1 (Mcg marker).
8	701	Hs.274772	Ribosomal L24 domain containing 1.
6	526	Hs.696211	Major histocompatibility complex, class II, DR beta 1.
5	438	Hs.730607	Non-SMC condensin II complex, subunit H2.
5	438	Hs.655575	Transcribed locus, strongly similar to NP_037124.1 Npm1 gene product [Rattus norvegicus].
5	438	Hs.610137	Src kinase associated phosphoprotein 2.
5	438	Hs.518475	Eukaryotic translation initiation factor 4A2.
5	438	Hs.368160	Protocadherin gamma subfamily C, 3.
5	438	Hs.285354	MYC associated factor X.
5	438	Hs.119598	Ribosomal protein L3.
4	351	Hs.89545	Proteasome (prosome, macropain) subunit, beta type, 4.
4	351	Hs.731111	Phosphodiesterase 4D interacting protein.
4	351	Hs.68061	Sphingosine kinase 1.
4	351	Hs.595983	Inositol hexakisphosphate kinase 2.
4	351	Hs.584927	YY1 associated protein 1.
4	351	Hs.524390	Tubulin, alpha 1b.
4	351	Hs.444467	Eukaryotic translation elongation factor 1 gamma.
4	351	Hs.435302	Zinc finger protein 3.
4	351	Hs.421848	Mitochondrial ribosomal protein L43.
4	351	Hs.412468	Kelch domain containing 3.
4	351	Hs.405590	Eukaryotic translation initiation factor 3, subunit E.
4	351	Hs.348308	Family with sequence similarity 189, member B.
4	351	Hs.255093	Phosphofructokinase, liver.
4	351	Hs.155218	Heterogeneous nuclear ribonucleoprotein U-like 1.
4	351	Hs.12970	Proteasome (prosome, macropain) 26S subunit, non-ATPase, 3.
3	263	Hs.96885	Vasohibin 2.
3	263	Hs.87469	Acid-sensing (proton-gated) ion channel family member 4.
3	263	Hs.808	Heterogeneous nuclear ribonucleoprotein F.
3	263	Hs.79322	Glutaminyl-tRNA synthetase.
3	263	Hs.7886	Pellino E3 ubiquitin protein ligase 1.
3	263	Hs.74647	YME1-like 1 (S. cerevisiae).
3	263	Hs.744897	Ribosomal protein L6.
3	263	Hs.743218	Major histocompatibility complex, class I, C.
3	263	Hs.741179	Serine hydroxymethyltransferase 2 (mitochondrial).
3	263	Hs.731375	TCR gamma alternate reading frame protein.
3	263	Hs.730696	RAD51 associated protein 1.
3	263	Hs.728838	StAR-related lipid transfer (START) domain containing 3.
3	263	Hs.727543	Heat shock 60kDa protein 1 (chaperonin).
3	263	Hs.721470	Zinc finger and BTB domain containing 44.
3	263	Hs.719925	Retinol dehydrogenase 11 (all-trans/9-cis/11-cis).
3	263	Hs.709217	Complement factor H-related 3.
3	263	Hs.673850	G protein-coupled receptor 52.
3	263	Hs.669893	Zinc finger protein 695.
3	263	Hs.666099	Family with sequence similarity 71, member B.
3	263	Hs.659606	Acyl-CoA synthetase medium-chain family member 5.
3	263	Hs.658676	Single stranded DNA binding protein 3.
3	263	Hs.654700	Zinc finger, CCHC domain containing 7.
3	263	Hs.654552	KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 2.
3	263	Hs.654350	GA binding protein transcription factor, beta subunit 1.
3	263	Hs.652390	Tubulin, alpha 1c.
3	263	Hs.648590	Transcribed locus, strongly similar to NP_001018079.1 SERBP1 gene product [Homo sapiens].
3	263	Hs.645535	Gamma-glutamyltransferase 1.
3	263	Hs.645517	KRR1, small subunit (SSU) processome component, homolog (yeast).
3	263	Hs.6449	Cleavage and polyadenylation specific factor 3-like.
3	263	Hs.643452	DiGeorge syndrome critical region gene 8.
3	263	Hs.635313	Charged multivesicular body protein 5.
3	263	Hs.633853	Glucosamine-6-phosphate deaminase 1.
3	263	Hs.632988	Transcribed locus, strongly similar to NP_000137.2 FTL gene product [Homo sapiens].
3	263	Hs.615092	Nucleolar and spindle associated protein 1.
3	263	Hs.61232	Interleukin 20 receptor beta.
3	263	Hs.594923	Hydroxysteroid (17-beta) dehydrogenase 11.
3	263	Hs.594238	Karyopherin alpha 2 (RAG cohort 1, importin alpha 1).
3	263	Hs.594084	Ankyrin repeat and KH domain containing 1.
3	263	Hs.593928	Farnesyl-diphosphate farnesyltransferase 1.
3	263	Hs.592145	WNT1 inducible signaling pathway protein 2.
3	263	Hs.584760	Guanine nucleotide binding protein (G protein), alpha z polypeptide.
3	263	Hs.584748	CDC-like kinase 3.
3	263	Hs.573007	Solute carrier family 41, member 3.
3	263	Hs.569006	Transcribed locus, strongly similar to NP_054864.3 TMEM230 gene product [Homo sapiens].
3	263	Hs.567699	Transcribed locus, strongly similar to NP_003964.3 RPL14 gene product [Homo sapiens].
3	263	Hs.565094	LSM domain containing 1.
3	263	Hs.554892	SprT-like N-terminal domain.
3	263	Hs.554822	UDP glucuronosyltransferase 1 family, polypeptide A1.
3	263	Hs.534360	Thyroid hormone receptor interactor 6.
3	263	Hs.533709	Berardinelli-Seip congenital lipodystrophy 2 (seipin).
3	263	Hs.533013	Cystathionine-beta-synthase.
3	263	Hs.531785	HCLS1 binding protein 3.
3	263	Hs.525063	Transmembrane protein 126B.
3	263	Hs.524947	Cell division cycle 20.
3	263	Hs.523841	Aldehyde dehydrogenase 3 family, member B1.
3	263	Hs.523302	Peroxiredoxin 3.
3	263	Hs.522729	Armadillo repeat containing, X-linked 5.
3	263	Hs.517817	Transmembrane protein 43.
3	263	Hs.517207	Transcribed locus, strongly similar to NP_032933.1 peptidyl-prolyl cis-trans isomerase A [Mus musculus].
3	263	Hs.517172	Death inducer-obliterator 1.
3	263	Hs.515951	SH3 domain containing, Ysc84-like 1 (S. cerevisiae).
3	263	Hs.515846	RuvB-like 2 (E. coli).
3	263	Hs.514276	Sp2 transcription factor.
3	263	Hs.513267	Transducin (beta)-like 3.
3	263	Hs.512914	N(alpha)-acetyltransferase 16, NatA auxiliary subunit.
3	263	Hs.507290	Kelch domain containing 9.
3	263	Hs.506815	Transmembrane protein 116.
3	263	Hs.505824	Sorting and assembly machinery component 50 homolog (S. cerevisiae).
3	263	Hs.500156	DnaJ (Hsp40) homolog, subfamily C, member 7.
3	263	Hs.500015	Cyclin-dependent kinase 5, regulatory subunit 1 (p35).
3	263	Hs.496666	Septin 6.
3	263	Hs.496068	Cyclin-dependent kinase 16.
3	263	Hs.495250	Cysteine conjugate-beta lyase, cytoplasmic.