adult testis ORESTES (TLL)

Library Description

Organism:	Danio rerio
Strain:	Toh (Singaporean strain)
Developmental stage:	adult (fully mature)
Sex:	male
Organ:	pooled testis
Vector:	pGEM-T

ORESTES libraries were generated according to Neto et al. (Gene 186: 135-142, 1997). mRNA isolated from adult testis was reverse transcribed and PCR amplified using oligonucleotide primers designed for AFLP analysis or for amplification of specific genes from Arabidopsis, yeast or rice. The amplification products with a smear or multiple bands (> 500 bp) were then cloned into pGEM-T vector (Promega) and transformed into XL-10 Gold competent cell (Stratagene). The insert from randomly selected white colonies was PCR amplified using M13 forward and reverse primers and partially sequenced by using M13 reverse primer.

Source: Laszlo ORBAN, Temasek Lifesciences Laboratory

Gene Content Analysis

2,860 ESTs from this library were grouped into 1,204 UniGene entries (putative genes) [UniGene build #126, 19-Jun-2012]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
97	33916	Dr.142223	F-box and leucine-rich repeat protein 14a.
55	19231	Dr.75922	Prosaposin.
42	14685	Dr.16120	Prostaglandin E synthase 2-like.
40	13986	Dr.108277	B-cell receptor-associated protein 29.
39	13636	Dr.119351	Transcribed locus, strongly similar to NP_038951.1 E3 ubiquitin-protein ligase RNF19A [Mus musculus].
38	13287	Dr.82450	Transcribed locus, strongly similar to XP_002665372.2 PREDICTED: DNA topoisomerase 1 [Danio rerio].
35	12238	Dr.42290	RIB43A domain with coiled-coils 1.
29	10140	Dr.76815	Telomeric repeat binding factor a.
22	7692	Dr.75312	Eukaryotic translation initiation factor 5B.
22	7692	Dr.162099	Transcribed locus, moderately similar to NP_571780.1 major histocompatibility complex class I UEA precursor [Danio rerio].
22	7692	Dr.157030	Transcribed locus, strongly similar to XP_682709.5 PREDICTED: coiled-coil domain-containing protein 39 isoform 1, partial [Danio rerio].
21	7343	Dr.5206	Aldehyde dehydrogenase 1 family, member A2.
20	6993	Dr.76244	Eukaryotic translation initiation factor 6.
18	6294	Dr.88043	Transcribed locus, strongly similar to NP_001093520.2 muscleblind-like protein 1 isoform 2 [Danio rerio].
16	5594	Dr.84109	Non-SMC condensin I complex, subunit G.
16	5594	Dr.60628	Im:6907928.
16	5594	Dr.103514	Transcribed locus.
15	5245	Dr.90933	Zgc:171573.
15	5245	Dr.76942	Structural maintenance of chromosomes 1A, like.
15	5245	Dr.57903	Transcribed locus, strongly similar to XP_003199460.1 PREDICTED: protein timeless homolog [Danio rerio].
14	4895	Dr.92449	5-hydroxytryptamine (serotonin) receptor 1A a.
14	4895	Dr.28550	Protein tyrosine phosphatase type IVA, member 1.
14	4895	Dr.146171	Transcribed locus, weakly similar to XP_003453063.1 PREDICTED: solute carrier family 22 member 6-like [Oreochromis niloticus].
13	4545	Dr.9044	Myosin, heavy polypeptide 9a, non-muscle.
13	4545	Dr.85361	Golgin A7 family, member Ba.
13	4545	Dr.78093	Zgc:56178.
13	4545	Dr.20146	Bromodomain, testis-specific.
13	4545	Dr.118811	FYN oncogene related to SRC, FGR, YES a.
13	4545	Dr.107256	Zgc:136639.
13	4545	Dr.106932	Transcribed locus, strongly similar to XP_002665521.2 PREDICTED: zinc finger protein 836 [Danio rerio].
13	4545	Dr.106286	Transcribed locus, strongly similar to XP_707330.1 PREDICTED: cytosolic 5'-nucleotidase 1A-like isoform 3 [Danio rerio].
12	4196	Dr.85231	Vacuolar protein sorting 4b (yeast).
12	4196	Dr.77201	Carboxypeptidase A5.
12	4196	Dr.18697	Transcribed locus, strongly similar to XP_687726.1 PREDICTED: coiled-coil domain-containing protein 36-like [Danio rerio].
12	4196	Dr.18530	Family with sequence similarity 166, member B.
11	3846	Dr.44360	Sestrin 2.
11	3846	Dr.34606	MYB binding protein (P160) 1a.
11	3846	Dr.34125	NADH dehydrogenase (ubiquinone) flavoprotein 1.
11	3846	Dr.20951	Synaptophysin-like 2a.
10	3497	Dr.83749	Tudor domain containing 3.
10	3497	Dr.75558	Solute carrier family 4, anion exchanger, member 2a.
10	3497	Dr.6092	HIRA interacting protein 3.
10	3497	Dr.150941	Transcribed locus.
9	3147	Dr.41821	Proteolipid protein 2.
9	3147	Dr.37160	Spire homolog 1a (Drosophila).
9	3147	Dr.32630	Nucleosome assembly protein 1, like 1.
9	3147	Dr.21458	Transcribed locus.
9	3147	Dr.160229	Transcribed locus, strongly similar to XP_001922198.2 PREDICTED: bromodomain and PHD finger-containing protein 3-like [Danio rerio].
9	3147	Dr.15285	Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3bl.
9	3147	Dr.119138	Transcribed locus.
8	2797	Dr.83478	Zgc:63882.
8	2797	Dr.79621	Pseudouridylate synthase 7 homolog (S. cerevisiae)-like.
8	2797	Dr.76240	Far upstream element (FUSE) binding protein 1.
8	2797	Dr.51730	Proteasome (prosome, macropain) 26S subunit, non-ATPase, 12.
8	2797	Dr.42030	Transcribed locus, weakly similar to XP_002665521.2 PREDICTED: zinc finger protein 836 [Danio rerio].
8	2797	Dr.155600	Phoenix.
8	2797	Dr.150255	Transcribed locus, strongly similar to XP_002664782.2 PREDICTED: protein IWS1 homolog [Danio rerio].
8	2797	Dr.115147	Transcribed locus, strongly similar to XP_001337035.4 PREDICTED: hypothetical protein LOC799358 [Danio rerio].
8	2797	Dr.11330	CDNA clone IMAGE:5915588.
8	2797	Dr.108520	DDB1 and CUL4 associated factor 12.
7	2448	Dr.94932	Transcribed locus.
7	2448	Dr.85513	Si:dkeyp-35b8.5.
7	2448	Dr.82470	ATG13 autophagy related 13 homolog (S. cerevisiae).
7	2448	Dr.79216	Replication factor C (activator 1) 1.
7	2448	Dr.78251	COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis).
7	2448	Dr.76519	Guanine nucleotide binding protein-like 3 (nucleolar).
7	2448	Dr.75585	Transformer-2 alpha.
7	2448	Dr.75152	Cyclin-dependent kinase 2.
7	2448	Dr.688	Protein phosphatase 4, regulatory subunit 2b.
7	2448	Dr.64200	Dynein, axonemal, heavy chain 7.
7	2448	Dr.47275	Heterogeneous nuclear ribonucleoprotein K, like.
7	2448	Dr.2409	TAR DNA binding protein.
7	2448	Dr.19771	Intraflagellar transport 172.
7	2448	Dr.159472	Transcribed locus, strongly similar to XP_002663993.1 PREDICTED: ATP-dependent RNA helicase DHX8 isoform 1 [Danio rerio].
7	2448	Dr.133616	Structural maintenance of chromosomes 1B.
7	2448	Dr.132728	Euchromatic histone-lysine N-methyltransferase 1b.
7	2448	Dr.122692	Transcribed locus.
7	2448	Dr.122415	CDNA clone IMAGE:7015027.
7	2448	Dr.116044	Si:dkeyp-110g5.4.
6	2098	Dr.9125	Synaptotagmin XIa.
6	2098	Dr.8966	Vacuolar protein sorting protein 18.
6	2098	Dr.81780	Proliferation associated nuclear element.
6	2098	Dr.78589	RAP1 interacting factor homolog (yeast).
6	2098	Dr.75526	Guanine nucleotide binding protein (G protein), beta polypeptide 1a.
6	2098	Dr.73935	Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10a.
6	2098	Dr.70084	Leber congenital amaurosis 5.
6	2098	Dr.5568	Alpha thalassemia/mental retardation syndrome X-linked homolog (human).
6	2098	Dr.44287	Transcribed locus, strongly similar to XP_002662821.2 PREDICTED: hypothetical protein LOC569661 isoform 1 [Danio rerio].
6	2098	Dr.4248	Zgc:55461.
6	2098	Dr.24937	Structural maintenance of chromosomes 3.
6	2098	Dr.2357	Mannosidase, alpha, class 2B, member 2.
6	2098	Dr.162073	Zona pellucida glycoprotein 2.
6	2098	Dr.154622	Transcribed locus, strongly similar to XP_001919161.1 PREDICTED: hypothetical protein LOC100150702 [Danio rerio].
6	2098	Dr.151172	Transcribed locus, strongly similar to NP_001018436.1 poly(A) RNA polymerase GLD2 [Danio rerio].
6	2098	Dr.111381	Glucosamine-6-phosphate isomerase 2-like.
6	2098	Dr.105104	Transcribed locus, strongly similar to XP_001340467.2 PREDICTED: hypothetical protein LOC100000219 [Danio rerio].
5	1748	Dr.9743	High-mobility group box 2b.
5	1748	Dr.92384	Uncharacterized LOC100149090.
5	1748	Dr.91295	Zgc:101737.
5	1748	Dr.8658	M-phase phosphoprotein 8.