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An organized view of the transcripome



Library:14182 (dbEST ID)

NIH_ZGC_7

Library Description

Organism:Danio rerio
Tissue:whole body
Vector:pExpress1
Vector type:phagemid (ampicillin resistant)
Cloning site 1:NotI
Cloning site 2:EcoRV
Lab host:DH10B

Bulk tissue was collected from a whole adult individual from the Tuebingen strain. 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was cloned into the Not I and EcoRV sites of pExpress-1. Library was size-selected for >1 kb fragments and normalized. A non-normalized version of this library is also available (NIH_ZGC_10). Library was constructed by Open Biosystems (Huntsville, AL)

Source: Daniela S. Gerhard, Ph.D., National Cancer Institute / NIH

Gene Content Analysis

20,020 ESTs from this library were grouped into 8,235 UniGene entries (putative genes) [UniGene build #126, 19-Jun-2012]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs TPM   UniGene Entry
55 2747 Dr.33333 Transcribed locus, strongly similar to NP_059333.1 cytochrome c oxidase subunit I [Danio rerio].
43 2148 Dr.935 S-adenosylhomocysteine hydrolase.
26 1299 Dr.124118 Transcribed locus.
25 1249 Dr.162030 Keratin 5.
24 1199 Dr.13436 Uncharacterized LOC796447.
22 1099 Dr.132310 Acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain.
21 1049 Dr.6431 Suppressor of cytokine signaling 3a.
21 1049 Dr.31797 Eukaryotic translation elongation factor 1 alpha 1, like 1.
21 1049 Dr.24246 H1 histone family, member X.
21 1049 Dr.2413 Dual specificity phosphatase 1.
21 1049 Dr.104720 Apolipoprotein A-II.
20 999 Dr.77127 Zgc:66382.
20 999 Dr.76749 Cd63 antigen.
20 999 Dr.17331 Zgc:174863.
20 999 Dr.104534 Uncharacterized protein LOC795545.
19 949 Dr.79369 Zgc:56719.
19 949 Dr.76309 Aldolase b, fructose-bisphosphate.
19 949 Dr.76034 Myoglobin.
19 949 Dr.75522 Glutamic-oxaloacetic transaminase 1, soluble.
18 899 Dr.77654 Secreted immunoglobulin domain 4.
18 899 Dr.75669 Parvalbumin 4.
18 899 Dr.55617 Ribosomal protein, large, P0.
18 899 Dr.51646 Beta-2-microglobulin.
18 899 Dr.4077 Non-metastatic cells 2b.2, protein (NM23B) expressed in.
18 899 Dr.30295 Transcribed locus, strongly similar to NP_989562.2 ADP/ATP translocase 3 [Gallus gallus].
18 899 Dr.16380 Aldehyde dehydrogenase 8 family, member A1.
18 899 Dr.162096 Zgc:77778.
18 899 Dr.106159 Zgc:162730.
17 849 Dr.7740 CD74 molecule, major histocompatibility complex, class II invariant chain a.
17 849 Dr.76600 ChaC, cation transport regulator-like 1.
17 849 Dr.75743 Cystatin C (amyloid angiopathy and cerebral hemorrhage).
16 799 Dr.79210 Glycerol-3-phosphate dehydrogenase 1b.
16 799 Dr.76290 Ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1.
16 799 Dr.75775 Apolipoprotein A-Ia.
16 799 Dr.59019 Y box binding protein 1.
16 799 Dr.32351 Lectin, galactoside-binding, soluble, 3 (galectin 3)-like.
16 799 Dr.24504 Poly A binding protein, cytoplasmic 1 a.
16 799 Dr.162032 Zgc:92533.
16 799 Dr.152450 Actin, beta 2.
16 799 Dr.1280 CCAAT/enhancer binding protein (C/EBP), delta.
15 749 Dr.87300 CD36 antigen.
15 749 Dr.80068 Purine nucleoside phosphorylase 4b.
15 749 Dr.77046 Apolipoprotein C-I like.
15 749 Dr.75658 Transferrin-a.
15 749 Dr.75547 Trypsin.
15 749 Dr.43659 Nucleophosmin 1a.
15 749 Dr.37870 Creatine kinase, muscle b.
15 749 Dr.35640 Glyceraldehyde-3-phosphate dehydrogenase.
15 749 Dr.159462 Fructose-1,6-bisphosphatase 1b.
14 699 Dr.84875 Zgc:92249.
14 699 Dr.8435 Chromatin modifying protein 1B.
14 699 Dr.78121 Zgc:64106.
14 699 Dr.77989 Dual specificity phosphatase 5.
14 699 Dr.77226 Transcribed locus.
14 699 Dr.76813 NADH dehydrogenase (ubiquinone) Fe-S protein 8a.
14 699 Dr.75997 Annexin A2a.
14 699 Dr.75607 Voltage-dependent anion channel 3.
14 699 Dr.3613 Ceruloplasmin.
14 699 Dr.23445 Ba1 globin.
14 699 Dr.15359 Parvalbumin 3.
14 699 Dr.1291 SMT3 suppressor of mif two 3 homolog 3a.
14 699 Dr.105154 Glyoxylate reductase/hydroxypyruvate reductase b.
14 699 Dr.104840 Complement factor H like 4.
14 699 Dr.104721 Fatty acid binding protein 10a, liver basic.
13 649 Dr.9829 Phosphodiesterase 6G, cGMP-specific, rod, gamma.
13 649 Dr.77160 Cytochrome P450, family 3, subfamily A, polypeptide 65.
13 649 Dr.77141 Sorting nexin 3.
13 649 Dr.76397 Matrix metalloproteinase 2.
13 649 Dr.76266 Proteasome activator subunit 2.
13 649 Dr.75633 Zgc:77052.
13 649 Dr.75552 Actin, alpha, cardiac muscle 1b.
13 649 Dr.31902 Zgc:92041.
13 649 Dr.2784 Solute carrier family 25 (carnitine/acylcarnitine translocase), member 20.
13 649 Dr.26749 Chaperonin containing TCP1, subunit 3 (gamma).
13 649 Dr.149289 Zgc:174379.
13 649 Dr.125513 Transcribed locus, strongly similar to XP_002667589.2 PREDICTED: mucin-2-like [Danio rerio].
13 649 Dr.115574 CDNA clone IMAGE:6794776.
13 649 Dr.1054 Ribosomal protein S14.
13 649 Dr.104772 Zgc:195154.
13 649 Dr.104595 Transcribed locus, strongly similar to XP_001339274.1 PREDICTED: hypothetical protein LOC798849 [Danio rerio].
13 649 Dr.104472 CDNA clone IMAGE:7088011.
12 599 Dr.9860 Malate dehydrogenase 1b, NAD (soluble).
12 599 Dr.85174 Cathepsin L.1.
12 599 Dr.82774 Saccharopine dehydrogenase b.
12 599 Dr.82044 Cytochrome P450, family 8, subfamily B, polypeptide 3.
12 599 Dr.8194 Opsin 1 (cone pigments), short-wave-sensitive 1.
12 599 Dr.81647 Transcribed locus, strongly similar to XP_688258.2 PREDICTED: zinc finger protein 227-like [Danio rerio].
12 599 Dr.81043 Dual specificity phosphatase 2.
12 599 Dr.78525 Fucosidase, alpha-L- 2, plasma.
12 599 Dr.76663 Solute carrier family 25, member 33.
12 599 Dr.76367 Hypoxia induced gene 1.
12 599 Dr.76274 Phenylalanine hydroxylase.
12 599 Dr.75974 PDZ domain containing 1.
12 599 Dr.75683 Transmembrane emp24 domain trafficking protein 2.
12 599 Dr.75517 Ribosomal protein L34.
12 599 Dr.75124 Wu:fb18c02.
12 599 Dr.737 Jun B proto-oncogene b.
12 599 Dr.4850 Ring finger protein 128a.
12 599 Dr.3780 Microtubule-associated protein 1 light chain 3 alpha.
12 599 Dr.32972 Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase.

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Source: NCBI UniGene

EST Sequences

23,638 EST sequences from this library have been submitted to the public sequence database as of 19-Jun-2012.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

  • whole body
  • adult
  • normalized
  • bulk

Source: NCBI Clone Registry



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