UI-CF-DU0

Library Description

Organism:	Homo sapiens
Developmental stage:	Adult
Organ:	Lung
Tissue:	Primary Lung Epithelial Cells
Vector:	pT7T3-Pac (Pharmacia) with a modified polylinker
Vector type:	Plasmid
Cloning site 1:	EcoR I
Cloning site 2:	Not I
Lab host:	DH10B (Life Technologies) (T1 phage resistant)

UI-CF-DU0 is a cDNA library containing the following tissue(s): Primary Lung Epithelial Cells The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GGCTGTAGGC.

Source: McCray, PB, University of Iowa

Gene Content Analysis

959 ESTs from this library were grouped into 787 UniGene entries (putative genes) [UniGene build #236, 09-Mar-2013]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
9	9385	Hs.497589	Polymeric immunoglobulin receptor.
8	8342	Hs.525600	Heat shock protein 90kDa alpha (cytosolic), class A member 1.
7	7299	Hs.535192	Eukaryotic translation elongation factor 1 alpha 1.
6	6257	Hs.8102	Ribosomal protein S20.
6	6257	Hs.604001	Heterogeneous nuclear ribonucleoprotein H1 (H).
6	6257	Hs.433529	Ribosomal protein S11.
6	6257	Hs.291196	ATPase, Na+/K+ transporting, beta 1 polypeptide.
5	5214	Hs.621695	Metastasis associated lung adenocarcinoma transcript 1 (non-protein coding).
5	5214	Hs.534255	Beta-2-microglobulin.
5	5214	Hs.520640	Actin, beta.
4	4171	Hs.514196	Ribosomal protein L27.
4	4171	Hs.494173	Annexin A1.
4	4171	Hs.459927	Prothymosin, alpha.
4	4171	Hs.28491	Spermidine/spermine N1-acetyltransferase 1.
4	4171	Hs.280378	Small nuclear ribonucleoprotein polypeptide B.
4	4171	Hs.201446	PERP, TP53 apoptosis effector.
3	3128	Hs.76392	Aldehyde dehydrogenase 1 family, member A1.
3	3128	Hs.743965	Transmembrane BAX inhibitor motif containing 6.
3	3128	Hs.743241	Heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa).
3	3128	Hs.730597	Ribosomal protein L9.
3	3128	Hs.672696	Transcribed locus.
3	3128	Hs.624	Interleukin 8.
3	3128	Hs.527948	Transcribed locus.
3	3128	Hs.523262	Transmembrane protein 59.
3	3128	Hs.513851	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide.
3	3128	Hs.408073	Ribosomal protein S6.
3	3128	Hs.295626	Transcribed locus.
3	3128	Hs.227948	Serpin peptidase inhibitor, clade B (ovalbumin), member 3.
3	3128	Hs.180909	Peroxiredoxin 1.
2	2086	Hs.89545	Proteasome (prosome, macropain) subunit, beta type, 4.
2	2086	Hs.79101	Cyclin G1.
2	2086	Hs.745122	Eukaryotic translation elongation factor 1 alpha 1.
2	2086	Hs.744479	Transcribed locus.
2	2086	Hs.731519	Wiskott-Aldrich syndrome-like.
2	2086	Hs.726012	H3 histone, family 3A.
2	2086	Hs.716464	Beclin 1, autophagy related.
2	2086	Hs.709003	Transcribed locus.
2	2086	Hs.707308	Transcribed locus.
2	2086	Hs.706482	Transcribed locus.
2	2086	Hs.702447	Transcribed locus.
2	2086	Hs.700831	Transcribed locus.
2	2086	Hs.685374	Transcribed locus.
2	2086	Hs.675564	Dynactin 4 (p62).
2	2086	Hs.654599	Nucleobindin 2.
2	2086	Hs.654581	Phosphoribosyl pyrophosphate synthetase 2.
2	2086	Hs.644000	Clathrin interactor 1.
2	2086	Hs.622033	Transcribed locus.
2	2086	Hs.591865	Leucine rich repeat containing 6.
2	2086	Hs.591768	Basic transcription factor 3.
2	2086	Hs.586423	Eukaryotic translation elongation factor 1 alpha 1.
2	2086	Hs.567256	Aldo-keto reductase family 1, member C2.
2	2086	Hs.558476	DnaJ (Hsp40) homolog, subfamily C, member 2.
2	2086	Hs.535026	Transcribed locus, strongly similar to NP_006588.1 HSPA8 gene product [Homo sapiens].
2	2086	Hs.529059	Eukaryotic translation initiation factor 3, subunit G.
2	2086	Hs.522114	Clathrin, light chain A.
2	2086	Hs.520414	Interferon gamma receptor 1.
2	2086	Hs.518525	Glutamate-ammonia ligase.
2	2086	Hs.517070	Secretory leukocyte peptidase inhibitor.
2	2086	Hs.515162	Calreticulin.
2	2086	Hs.512675	Ribosomal protein S8.
2	2086	Hs.510402	CD46 molecule, complement regulatory protein.
2	2086	Hs.507680	NEDD4 binding protein 2-like 2.
2	2086	Hs.506759	ATPase, Ca++ transporting, cardiac muscle, slow twitch 2.
2	2086	Hs.501023	Transcribed locus.
2	2086	Hs.499145	YME1-like 1 (S. cerevisiae).
2	2086	Hs.482043	Nicotinamide nucleotide transhydrogenase.
2	2086	Hs.478275	Tumor necrosis factor (ligand) superfamily, member 10.
2	2086	Hs.475733	Topoisomerase (DNA) II beta 180kDa.
2	2086	Hs.468410	Endothelial PAS domain protein 1.
2	2086	Hs.467807	Lysosomal protein transmembrane 4 alpha.
2	2086	Hs.446079	Carboxypeptidase D.
2	2086	Hs.445586	Chemokine (C-X-C motif) ligand 17.
2	2086	Hs.445203	DnaJ (Hsp40) homolog, subfamily A, member 1.
2	2086	Hs.443661	Synaptojanin 2 binding protein.
2	2086	Hs.440833	Protein kinase N2.
2	2086	Hs.436687	SET nuclear oncogene.
2	2086	Hs.434993	DnaJ (Hsp40) homolog, subfamily C, member 27.
2	2086	Hs.433512	ARP3 actin-related protein 3 homolog (yeast).
2	2086	Hs.432940	Transcribed locus.
2	2086	Hs.429180	Eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa.
2	2086	Hs.422986	Annexin A4.
2	2086	Hs.406300	Ribosomal protein L23.
2	2086	Hs.401388	SUMO1/sentrin/SMT3 specific peptidase 2.
2	2086	Hs.397609	Ribosomal protein S16.
2	2086	Hs.376064	NOP56 ribonucleoprotein.
2	2086	Hs.368938	Chromosome 12 open reading frame 75.
2	2086	Hs.356572	Ribosomal protein S3A.
2	2086	Hs.347991	Nuclear receptor subfamily 2, group F, member 2.
2	2086	Hs.309641	Ring finger protein 11.
2	2086	Hs.303676	GTPase activating protein (SH3 domain) binding protein 2.
2	2086	Hs.279806	DEAD (Asp-Glu-Ala-Asp) box helicase 5.
2	2086	Hs.278429	Chromosome 9 open reading frame 78.
2	2086	Hs.25155	Neuroepithelial cell transforming 1.
2	2086	Hs.250456	DEAH (Asp-Glu-Ala-His) box polypeptide 33.
2	2086	Hs.23582	Tumor-associated calcium signal transducer 2.
2	2086	Hs.201641	Brain abundant, membrane attached signal protein 1.
2	2086	Hs.196133	Transcribed locus.
2	2086	Hs.187866	Transcribed locus.
2	2086	Hs.180877	H3 histone, family 3B (H3.3B).
2	2086	Hs.180414	Heat shock 70kDa protein 8.