UI-E-CK1

Library Description

Organism:	Homo sapiens
Developmental stage:	adult
Organ:	eye
Tissue:	Retina Foveal and Macular
Vector:	pT7T3-Pac (Pharmacia) with a modified polylinker
Vector type:	Plasmid
Cloning site 1:	EcoR I
Cloning site 2:	Not I
Lab host:	DH10B (Life Technologies) (T1 phage resistant)

UI-E-CK1 is a normalized cDNA library containing the following tissue(s): Retina Foveal and Macular. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GTCC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

Source: Soares, MB, Children's Memorial Research Center

Gene Content Analysis

6,226 ESTs from this library were grouped into 3,158 UniGene entries (putative genes) [UniGene build #236, 09-Mar-2013]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
46	7388	Hs.518267	Transferrin.
15	2409	Hs.247565	Rhodopsin.
14	2249	Hs.521461	Neurofilament, light polypeptide.
13	2088	Hs.591928	Retinol binding protein 3, interstitial.
12	1927	Hs.419463	Ribosomal protein L23a.
11	1767	Hs.709709	Neuronal differentiation 1.
11	1767	Hs.400295	Ribosomal protein L30.
10	1606	Hs.637850	Reticulon 4.
10	1606	Hs.374503	Mortality factor 4 like 1.
9	1446	Hs.92858	Guanylate cyclase activator 1A (retina).
9	1446	Hs.90297	Synuclein, beta.
9	1446	Hs.525600	Heat shock protein 90kDa alpha (cytosolic), class A member 1.
9	1446	Hs.448941	Family with sequence similarity 71, member E1.
9	1446	Hs.268849	Glyoxalase I.
9	1446	Hs.150580	Eukaryotic translation initiation factor 1.
9	1446	Hs.125898	GNAS complex locus.
8	1285	Hs.80539	Recoverin.
8	1285	Hs.446574	Thymosin beta 10.
8	1285	Hs.441664	Tetraspanin 7.
8	1285	Hs.356331	Peptidylprolyl isomerase A (cyclophilin A).
8	1285	Hs.162233	Chromodomain helicase DNA binding protein 4.
7	1124	Hs.7946	Microtubule associated tumor suppressor 1.
7	1124	Hs.643540	ATPase, Na+/K+ transporting, beta 2 polypeptide.
7	1124	Hs.533222	Transcribed locus.
7	1124	Hs.511605	Annexin A2.
7	1124	Hs.431668	Cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous).
7	1124	Hs.310545	Synaptotagmin I.
7	1124	Hs.283652	Isopentenyl-diphosphate delta isomerase 1.
7	1124	Hs.264208	Coiled-coil domain containing 104.
7	1124	Hs.260603	Phosphatidylinositol-5-phosphate 4-kinase, type II, beta.
7	1124	Hs.183706	Adducin 1 (alpha).
7	1124	Hs.128453	Frizzled-related protein.
6	964	Hs.744924	Ornithine decarboxylase antizyme 2.
6	964	Hs.719951	Ribosomal protein L11.
6	964	Hs.644397	Prenylcysteine oxidase 1 like.
6	964	Hs.632472	Regulatory factor X, 5 (influences HLA class II expression).
6	964	Hs.604915	Dystonin.
6	964	Hs.601206	Ankyrin repeat domain 17.
6	964	Hs.591024	Neuronal differentiation 4.
6	964	Hs.58471	Selenoprotein K.
6	964	Hs.534477	Ubiquitin-like 5.
6	964	Hs.534453	NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13.
6	964	Hs.524910	Ferritin, heavy polypeptide 1.
6	964	Hs.521651	Stathmin-like 2.
6	964	Hs.518731	Ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase).
6	964	Hs.514196	Ribosomal protein L27.
6	964	Hs.513490	Aldolase A, fructose-bisphosphate.
6	964	Hs.505104	Kelch-like family member 42.
6	964	Hs.501293	Basigin (Ok blood group).
6	964	Hs.483784	SH3 domain and tetratricopeptide repeats 2.
6	964	Hs.468442	Calmodulin 2 (phosphorylase kinase, delta).
6	964	Hs.467701	Ornithine decarboxylase 1.
6	964	Hs.445489	Pleckstrin homology domain containing, family B (evectins) member 1.
6	964	Hs.443914	Superoxide dismutase 1, soluble.
6	964	Hs.421509	Chaperonin containing TCP1, subunit 4 (delta).
6	964	Hs.375616	Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7.
6	964	Hs.368281	Microtubule-associated protein 2.
6	964	Hs.356502	Ribosomal protein, large, P1.
6	964	Hs.335079	Microtubule-associated protein 1B.
6	964	Hs.308074	Transmembrane protein 245.
6	964	Hs.284122	WNT inhibitory factor 1.
6	964	Hs.25348	Vesicle-associated membrane protein 2 (synaptobrevin 2).
6	964	Hs.21754	Synaptic vesicle glycoprotein 2B.
6	964	Hs.209249	Interphotoreceptor matrix proteoglycan 2.
6	964	Hs.175437	Erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked).
6	964	Hs.173438	Fas apoptotic inhibitory molecule.
6	964	Hs.132121	SH3-domain GRB2-like (endophilin) interacting protein 1.
6	964	Hs.117694	Calcium binding protein 5.
5	803	Hs.97858	Kinesin family member 1B.
5	803	Hs.8526	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1.
5	803	Hs.8008	DNA methyltransferase 1 associated protein 1.
5	803	Hs.743229	Reticulon 3.
5	803	Hs.732820	Retinitis pigmentosa 1 (autosomal dominant).
5	803	Hs.714318	Transcribed locus, strongly similar to XP_001513394.1 PREDICTED: eukaryotic initiation factor 4A-II-like, partial [Ornithorhynchus anatinus].
5	803	Hs.705699	Transmembrane protein 50A.
5	803	Hs.69855	Cold shock domain containing E1, RNA-binding.
5	803	Hs.68714	Serine/arginine-rich splicing factor 1.
5	803	Hs.66187	Clone 23700 mRNA sequence.
5	803	Hs.660805	Transcribed locus.
5	803	Hs.656185	Zinc finger protein 266.
5	803	Hs.643552	Short chain dehydrogenase/reductase family 39U, member 1.
5	803	Hs.633601	Ariadne homolog 2 (Drosophila).
5	803	Hs.632486	Myeloid cell leukemia sequence 1 (BCL2-related).
5	803	Hs.618732	WAS protein family, member 3.
5	803	Hs.595158	Zinc finger, RAN-binding domain containing 1.
5	803	Hs.567714	Major facilitator superfamily domain containing 4.
5	803	Hs.567530	CDNA FLJ32348 fis, clone PROST2007200.
5	803	Hs.567370	HIRA interacting protein 3.
5	803	Hs.528305	DEAD (Asp-Glu-Ala-Asp) box helicase 17.
5	803	Hs.526521	Malate dehydrogenase 1, NAD (soluble).
5	803	Hs.524899	Sin3A-associated protein, 18kDa.
5	803	Hs.523215	NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa.
5	803	Hs.520087	Protein kinase C and casein kinase substrate in neurons 1.
5	803	Hs.519659	Dihydropyrimidinase-like 3.
5	803	Hs.517978	Guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1.
5	803	Hs.517622	Sad1 and UNC84 domain containing 2.
5	803	Hs.517338	ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1.
5	803	Hs.517232	Peroxisomal biogenesis factor 19.
5	803	Hs.516802	Kinesin family member 1A.
5	803	Hs.5120	Dynein, light chain, LC8-type 1.