AEQU00000000.2 Python bivittatus
The Python bivittatus whole genome shotgun (WGS) project has the project accession AEQU00000000. This version of the project (02) has the accession number AEQU02000000, and consists of sequences AEQU02000001-AEQU02274243.
Background section: The Burmese python was obtained from the pet trade by Carl Franklin (Biological Curator for the University of Texas at Arlington Amphibian and Reptile Diversity Research Center). A voucher of the remaining specimen has been permanently cataloged in the collection at UTA. The snake was a female. All DNA used for genome sequencing was obtained from DNA extracted from snap-frozen liver tissue from this single animal. This assembly of Burmese python was composed of both Illumina instrument reads (frags, 3kbs, and 8kbs) and Roche 454 reads. A 20X input coverage of Illumina sequence reads were assembled using the Soap deNovo software (Beijing Genome Institute). The 454 reads were assembled with the Newbler assembler (Roche). The assemblies were merged using GAA (Yao et al 2011). The Soap assembly served as the reference assembly in the merge and represents the majority of the final assembly. This draft assembly was referred to as Burmese python assembly 5.0.2. This version has been gap filled and cleaned of contaminating contigs. The assembly is made up of a total of 39,115 scaffolds with an N50 scaffold length of 207,524 (N50 contig length was 10,658). The assembly spans 1.44Gb. For questions regarding this Python molurus bivittatus 5.0.2 assembly please contact Todd Castoe, University of Texas Arlington (firstname.lastname@example.org). Source DNA requests - address: Todd Castoe Univ. Texas Arlington 500 UTA Blvd. Engineering Research Bldg., Rm 334 Arlington, TX 76019 Credits: DNA source - Carl Franklin Biological Curator for the University of Texas at Arlington. Sequencing - The Genome Institute, Washington University School of Medicine, St Louis, MO, and the University of Colorado School of Medicine, Denver, CO. Sequence assembly - The Genome Institute, Washington University School of Medicine, St Louis, MO. Citation upon use of this assembly in a manuscript: It is requested that users of this Python molurus bivittatus 5.0.2 sequence assembly acknowledge Todd Castoe and the University of Texas at Arlington in any publications that result from use of this sequence assembly.