The Rickettsia endosymbiont of Ixodes scapularis whole genome shotgun (WGS) project has the project accession ACLC00000000. This version of the project (01) has the accession number ACLC01000000, and consists of sequences ACLC01000001-ACLC01000135.
The Rickettsia endosymbiont of Ixodes scapularis Wikel (REIS) genome was assembled at The J. Craig Venter Institute (JCVI, www.jcvi.org) and funded by the National Institute of Allergy and Infectious Diseases, NIH. This is a hybrid assembly that consists of shotgun sequences from I. scapularis BAC clones and recruited WGS reads of I. scapularis (accession ABJB000000000, ProjectID 16232). The assembly consists of 20 scaffolds including the main chromosome and 3 plasmids (CM000770-CM000773) plus 16 unplaced scaffolds (GG688301-GG688316), 9 of which are singletons. The draft chromosome of REIS is a scaffold containing 109 gaps of estimated sizes. Two of the plasmids, pREIS1 and pREIS2, have been circularized, while the third plasmid, pREIS3, has support for circularization but has not been circularized due to the presence of SNPs in the untrimmed overlap region.
The annotation for the REIS assembly was produced as a joint effort between JCVI, Institute for Genome Sciences (http://www.igs.umaryland.edu) and PATRIC (the PathoSystems Resource Integration Center at the Virginia Bioinformatics Institute, http://patric.vbi.vt.edu). The chromosome and 3 plasmids were annotated, with a total of 2117 protein coding genes, 123 pseudogenes and 45 non-coding RNA genes. Computational annotation methods were supplemented with manual review of some of the predicted genes. Long-term curation of the sequence and subsequent annotation updates will be the responsibility of PATRIC.
Genome Coverage: 10.7x
Sequencing Technology: ABI.
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