The Staphylococcus aureus subsp. aureus TCH130 whole genome shotgun (WGS) project has the project accession ACHD00000000. This version of the project (01) has the accession number ACHD01000000, and consists of sequences ACHD01000001-ACHD01000284.
This sequence is generated as a part of the Human Microbiome Project (HMP), funded by the National Institutes of Health (NIH), National Human Genome Research Institute (NHGRI) Large-Scale Sequencing Research Network and the National Institute of Allergy and Infectious Diseases (NIAID) Microbial Genome Sequencing Centers Program. The mission of the HMP is to generate resources to enable the characterization of the human microbiota, the microbial communities living within human bodies, and analyze the role of these organisms in human health and disease. See http://www.hmpdacc.org/ for more information.
The sequenced strain was obtained from Dr. Sheldon Kaplan (contact information: Texas Children's Hospital, Department of Pediatric Infectious Diseases, 1102 Bates Street #1150, Houston, TX, 77030; email: slkaplan@TexasChildrensHospital.org). The genomic DNA was prepared from a single bacterial isolate. The sequence generated included at least 10 fold coverage of Roche/454 Lifesciences FLX fragment data, at least 8 fold clone coverage of Roche/454 Lifesciences paired-end data and at least 10 fold coverage of Illumina/Solexa data.
The Roche/454 Lifesciences sequence was assembled using the Roche/454 Lifescience Newbler assembler. The contigs from the Newbler assembly were aligned to the Illumina/Solexa data with mapping tools such as Mosaic and Crossmatch and these data were used for error correction for this version of the draft assembly. This draft assembly meets the HMP draft quality standards (more than 90% of the genome is included in contigs, more than 90% of a core set of bacterial genes are found with > 30% identity and > 30% length; more than 90% of the bases in the assembly have more than 5 fold sequence coverage, the contig N50 length is greater than 5kb, the scaffold N50 length is greater than 20kb, and there is less than 1 gap per 5kb).
This is a reference genome for the Human Microbiome Project.
This project is co-owned with the Human Microbiome Project DACC.
Genome Coverage: 43x
Sequencing Technology: 454; ABI; Solexa/Illumina
Annotation was added to the contigs in July 2009.
Product names were updated in June 2013.
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