3DTU: Catalytic Core Subunits (I and II) of Cytochrome C Oxidase From Rhodobacter Sphaeroides Complexed With Deoxycholic Acid

Citation:
Abstract
Micromolar concentrations of the bile salt deoxycholate are shown to rescue the activity of an inactive mutant, E101A, in the K proton pathway of Rhodobacter sphaeroides cytochrome c oxidase. A crystal structure of the wild-type enzyme reveals, as predicted, deoxycholate bound with its carboxyl group at the entrance of the K path. Since cholate is a known potent inhibitor of bovine oxidase and is seen in a similar position in the bovine structure, the crystallographically defined, conserved steroid binding site could reveal a regulatory site for steroids or structurally related molecules that act on the essential K proton path.
PDB ID: 3DTUDownload
MMDB ID: 66770
PDB Deposition Date: 2008/7/15
Updated in MMDB: 2008/10 
Experimental Method:
x-ray diffraction
Resolution: 2.15  Å
Source Organism:
Similar Structures:
Biological Unit for 3DTU: dimeric; determined by software (PISA)
Molecular Components in 3DTU
Label Count Molecule
Proteins (2 molecules)
1
Cytochrome C Oxidase Subunit 1
Molecule annotation
1
Cytochrome C Oxidase Subunit 2
Molecule annotation
Chemicals (30 molecules)
1
7
2
3
3
1
4
1
5
1
6
1
7
2
8
10
9
3
10
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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