2YA4: Crystal Structure of Streptococcus Pneumoniae Nana (Tigr4)

Citation:
Abstract
The human pathogen Streptococcus pneumoniae is the major cause of bacterial meningitis, respiratory tract infection, septicemia, and otitis media. The bacterium expresses neuraminidase (NA) proteins that contribute to pathogenesis by cleaving sialic acids from host glycoconjugates, thereby enhancing biofilm formation and colonization. Recent in vivo experiments have shown that antiviral compounds, widely used in clinics and designed to inhibit influenza NA, significantly reduce biofilm formation and nasopharyngeal colonization of S. pneumoniae in mice. Here, we present the structural basis for the beneficial effect of these compounds against pneumococcal infection. Crystal structures of pneumococcal NanA in complex with zanamivir and oseltamivir carboxylate are discussed, correlated with measured inhibitory constants K(i), and compared with the binding modes of the inhibitors in the viral enzyme. Inhibitor structures show for the first time how clinically approved anti-influenza compounds interact with an NA of the human pathogen S. pneumoniae and give a rational explanation for their antibacterial effects.
PDB ID: 2YA4Download
MMDB ID: 90101
PDB Deposition Date: 2011/2/18
Updated in MMDB: 2011/06 
Experimental Method:
x-ray diffraction
Resolution: 1.8  Å
Source Organism:
Similar Structures:
Biological Unit for 2YA4: monomeric; determined by author and by software (PISA)
Molecular Components in 2YA4
Label Count Molecule
Protein (1 molecule)
1
Neuraminidase a
Molecule annotation
Chemicals (10 molecules)
1
6
2
3
3
1
* Click molecule labels to explore molecular sequence information.

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