2F5Q: Catalytically Inactive (E3q) Mutm Crosslinked to Oxog:c Containing DNA CC2

DNA glycosylases must interrogate millions of base pairs of undamaged DNA in order to locate and then excise one damaged nucleobase. The nature of this search process remains poorly understood. Here we report the use of disulfide cross-linking (DXL) technology to obtain structures of a bacterial DNA glycosylase, MutM, interrogating undamaged DNA. These structures, solved to 2.0 angstrom resolution, reveal the nature of the search process: The protein inserts a probe residue into the helical stack and severely buckles the target base pair, which remains intrahelical. MutM therefore actively interrogates the intact DNA helix while searching for damage.
PDB ID: 2F5QDownload
MMDB ID: 94156
PDB Deposition Date: 2005/11/26
Updated in MMDB: 2011/10 
Experimental Method:
x-ray diffraction
Resolution: 2.35  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 2F5Q: trimeric; determined by author
Molecular Components in 2F5Q
Label Count Molecule
Protein (1 molecule)
Formamidopyrimidine-dna Glycosidase
Molecule annotation
Nucleotides(2 molecules)
5'- D(*ap*gp*g*tp*ap*gp*ap*cp*cp*tp*gp*gp*ap*cp*gp*c)-3'
Molecule annotation
5'-d(*tp*gp*cp*g*tp*cp*cp*ap*(8og) P*gp*tp*cp*tp*ap*cp*c)-3'
Molecule annotation
Chemical (1 molecule)
* Click molecule labels to explore molecular sequence information.

Citing MMDB