1TWG: RNA Polymerase II Complexed With CTP

Citation:
Abstract
Binding of a ribonucleoside triphosphate to an RNA polymerase II transcribing complex, with base pairing to the template DNA, was revealed by X-ray crystallography. Binding of a mismatched nucleoside triphosphate was also detected, but in an adjacent site, inverted with respect to the correctly paired nucleotide. The results are consistent with a two-step mechanism of nucleotide selection, with initial binding to an entry (E) site beneath the active center in an inverted orientation, followed by rotation into the nucleotide addition (A) site for pairing with the template DNA. This mechanism is unrelated to that of single subunit RNA polymerases and so defines a new paradigm for the large, multisubunit enzymes. Additional findings from these studies include a third nucleotide binding site that may define the length of backtracked RNA; DNA double helix unwinding in advance of the polymerase active center; and extension of the diffraction limit of RNA polymerase II crystals to 2.3 A.
PDB ID: 1TWGDownload
MMDB ID: 30342
PDB Deposition Date: 2004/6/30
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 3.3  Å
Source Organism:
Similar Structures:
Biological Unit for 1TWG: decameric; determined by author and by software (PISA)
Molecular Components in 1TWG
Label Count Molecule
Proteins (10 molecules)
1
DNA-directed RNA Polymerase II Largest Subunit(Gene symbol: RPO21)
Molecule annotation
1
DNA-directed RNA Polymerase II 140 KDA Polypeptide(Gene symbol: RPB2)
Molecule annotation
1
DNA-directed RNA Polymerase II 45 KDA Polypeptide(Gene symbol: RPB3)
Molecule annotation
1
DNA-directed RNA Polymerases I, II, and III 27 KDA Polypeptide(Gene symbol: RPB5)
Molecule annotation
1
DNA-directed RNA Polymerases I, II, and III 23 KDA Polypeptide(Gene symbol: RPO26)
Molecule annotation
1
DNA-directed RNA Polymerases I, II, and III 14.5 KDA Polypeptide(Gene symbol: RPB8)
Molecule annotation
1
DNA-directed RNA Polymerase II 14.2 KDA Polypeptide(Gene symbol: RPB9)
Molecule annotation
1
DNA-directed RNA Polymerases I, II, and III 8.3 KDA Polypeptide(Gene symbol: RPB10)
Molecule annotation
1
DNA-directed RNA Polymerase II 13.6 KDA Polypeptide(Gene symbol: RPB11)
Molecule annotation
1
DNA-directed RNA Polymerases I, II, and III 7.7 KDA Polypeptide(Gene symbol: RPC10)
Molecule annotation
Chemicals (11 molecules)
1
2
2
8
3
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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