1E4Q: Solution structure of the human defensin hBD-2

Citation:
Abstract
Defensins are cationic and cysteine-rich peptides that play a crucial role in the host defense against microorganisms of many organisms by their capability to permeabilize bacterial membranes. The low sequence similarity among the members of the large mammalian beta-defensin family suggests that their antimicrobial activity is largely independent of their primary structure. To investigate to what extent these defensins share a similar fold, the structures of the two human beta-defensins, hBD-1 and hBD-2, as well as those of two novel murine defensins, termed mBD-7 and mBD-8, were determined by nuclear magnetic resonance spectroscopy. All four defensins investigated share a striking similarity on the level of secondary and tertiary structure including the lack of a distinct hydrophobic core, suggesting that the fold is mainly stabilized by the presence of three disulfide bonds. In addition to the overall shape of the molecules, the ratio of solvent-exposed polar and hydrophobic side chains is also very similar among the four defensins investigated. It is significant that beta-defensins do not exhibit a common pattern of charged and hydrophobic residues on the protein surface and that the beta-defensin-specific fold appears to accommodate a wide range of different amino acids at most sequence positions. In addition to the implications for the mode of biological defensin actions, these findings are of particular interest because beta-defensins have been suggested as lead compounds for the development of novel peptide antibiotics for the therapy of infectious diseases.
PDB ID: 1E4QDownload
MMDB ID: 18211
PDB Deposition Date: 2000/7/12
Updated in MMDB: 2001/12
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Biological Unit for 1E4Q: monomeric; determined by author
Molecular Components in 1E4Q
Label Count Molecule
Protein (1 molecule)
1
Beta-defensin 2(Gene symbol: DEFB4A)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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