3J1N: Cryo-em MAP of a Yeast Minimal Preinitiation Complex Interacting With the Mediator Head Module

Citation:
Abstract
Mediator, a large (21 polypeptides, MW approximately 1 MDa) complex conserved throughout eukaryotes, plays an essential role in control of gene expression by conveying regulatory signals that influence the activity of the preinitiation complex. However, the precise mode of interaction between Mediator and RNA polymerase II (RNAPII), and the mechanism of regulation by Mediator remain elusive. We used cryo-electron microscopy and reconstituted in vitro transcription assays to characterize a transcriptionally-active complex including the Mediator Head module and components of a minimum preinitiation complex (RNAPII, TFIIF, TFIIB, TBP, and promoter DNA). Our results reveal how the Head interacts with RNAPII, affecting its conformation and function.
PDB ID: 3J1NDownload
MMDB ID: 108035
PDB Deposition Date: 2012/3/29
Updated in MMDB: 2013/03
Experimental Method:
electron microscopy
Resolution: 16  Å
Source Organism:
Similar Structures:
Biological Unit for 3J1N: dodecameric; determined by author
Molecular Components in 3J1N
Label Count Molecule
Proteins (12 molecules)
1
DNA-directed RNA Polymerase II Subunit Rpb1
Molecule annotation
1
DNA-directed RNA Polymerase II Subunit Rpb2
Molecule annotation
1
DNA-directed RNA Polymerase II Subunit Rpb3
Molecule annotation
1
DNA-directed RNA Polymerase II Subunit Rpb4
Molecule annotation
1
DNA-directed RNA Polymerase II Subunit Rpabc1
Molecule annotation
1
DNA-directed RNA Polymerase II Subunit Rpabc2
Molecule annotation
1
DNA-directed RNA Polymerase II Subunit Rpb7
Molecule annotation
1
DNA-directed RNA Polymerase II Subunit Rpabc3
Molecule annotation
1
DNA-directed RNA Polymerase II Subunit Rpb9
Molecule annotation
1
DNA-directed RNA Polymerase II Subunit Rpabc5
Molecule annotation
1
DNA-directed RNA Polymerase II Subunit Rpb11
Molecule annotation
1
DNA-directed RNA Polymerase II Subunit Rpabc4
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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