2WB1: The Complete Structure of the Archaeal 13-subunit DNA- Directed RNA Polymerase

Citation:
Abstract
The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfolobus shibatae at 3.35 A resolution. The structure contains the full complement of subunits, including RpoG/Rpb8 and the equivalent of the clamp-head and jaw domains of the eukaryotic Rpb1. Furthermore, we have identified subunit Rpo13, an RNAP component in the order Sulfolobales, which contains a helix-turn-helix motif that interacts with the RpoH/Rpb5 and RpoA'/Rpb1 subunits. Its location and topology suggest a role in the formation of the transcription bubble.
PDB ID: 2WB1Download
MMDB ID: 72709
PDB Deposition Date: 2009/2/19
Updated in MMDB: 2011/05 
Experimental Method:
x-ray diffraction
Resolution: 3.52  Å
Source Organism:
Similar Structures:
Biological Unit for 2WB1: tridecameric; determined by software (PQS)
Molecular Components in 2WB1
Label Count Molecule
Proteins (13 molecules)
1
DNA-directed RNA Polymerase Rpo1n Subunit
Molecule annotation
1
DNA-directed RNA Polymerase Rpo1c Subunit
Molecule annotation
1
DNA-directed RNA Polymerase Rpo2 Subunit
Molecule annotation
1
DNA-directed RNA Polymeraserpo3 Subunit
Molecule annotation
1
DNA-directed RNA Polymerase Rpo4 Subunit
Molecule annotation
1
DNA-directed RNA Polymerase Rpo5 Subunit
Molecule annotation
1
DNA-directed RNA Polymerase Rpo6 Subunit
Molecule annotation
1
DNA-directed RNA Polymerase Rpo7 Subunit
Molecule annotation
1
DNA-directed RNA Polymerase Rpo8 Subunit
Molecule annotation
1
DNA-directed RNA Polymerase Rpo10 Subunit
Molecule annotation
1
DNA-directed RNA Polymerase Rpo11 Subunit
Molecule annotation
1
DNA-directed RNA Polymerase Rpo12 Subunit
Molecule annotation
1
DNA-directed RNA Polymerase Rpo13 Subunit
Molecule annotation
Chemicals (7 molecules)
1
5
2
1
3
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.