National Center for
1HFJ: Asparaginase From Erwinia Chrysanthemi, Hexagonal Form With Sulfate
Structures of two highly homologous bacterial L-asparaginases: a case of enantiomorphic space groups
Acta Crystallogr. D Biol. Crystallogr. (2001) 57 p.369-377» All references (3)
Quasi-enantiomorphic crystals of the Y25F mutant of Escherichia coli L-asparaginase and of the native Erwinia chrysanthemi L-asparaginase were obtained in the hexagonal space groups P6(5)22 and P6(1)22, respectively. The structures of these highly homologous enzymes were solved by molecular replacement and were refined with data extending to 2.2-2.5 A. These structures were compared with each other, as well as with other L-asparaginase structures previously observed with different crystal packing. It is concluded that the observed phenomenon, which is rare, was most likely to have arisen by chance.