3RTR: A Ring E3-substrate Complex Poised for Ubiquitin-like Protein Transfer: Structural Insights Into Cullin-ring Ligases

How RING E3 ligases mediate E2-to-substrate ubiquitin-like protein (UBL) transfer remains unknown. Here we address how the RING E3 RBX1 positions NEDD8's E2 (UBC12) and substrate (CUL1). We find that existing structures are incompatible with CUL1 NEDD8ylation and report a new conformation of RBX1 that places UBC12 adjacent to CUL1. We propose RING domain rotation as a general mechanism for UBL transfer for the largest family of E3s.
PDB ID: 3RTRDownload
MMDB ID: 92202
PDB Deposition Date: 2011/5/3
Updated in MMDB: 2011/08 
Experimental Method:
x-ray diffraction
Resolution: 3.21  Å
Source Organism:
Similar Structures:
Biological Unit for 3RTR: dimeric; determined by author and by software (PISA)
Molecular Components in 3RTR
Label Count Molecule
Proteins (2 molecules)
Molecule annotation
E3 Ubiquitin-protein Ligase Rbx1
Molecule annotation
Chemicals (3 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB