1U04: Crystal Structure of Full Length Argonaute From Pyrococcus Furiosus

Citation:
Abstract
Argonaute proteins and small interfering RNAs (siRNAs) are the known signature components of the RNA interference effector complex RNA-induced silencing complex (RISC). However, the identity of "Slicer," the enzyme that cleaves the messenger RNA (mRNA) as directed by the siRNA, has not been resolved. Here, we report the crystal structure of the Argonaute protein from Pyrococcus furiosus at 2.25 angstrom resolution. The structure reveals a crescent-shaped base made up of the amino-terminal, middle, and PIWI domains. The Piwi Argonaute Zwille (PAZ) domain is held above the base by a "stalk"-like region. The PIWI domain (named for the protein piwi) is similar to ribonuclease H, with a conserved active site aspartate-aspartate-glutamate motif, strongly implicating Argonaute as "Slicer." The architecture of the molecule and the placement of the PAZ and PIWI domains define a groove for substrate binding and suggest a mechanism for siRNA-guided mRNA cleavage.
PDB ID: 1U04Download
MMDB ID: 29092
PDB Deposition Date: 2004/7/12
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 2.25  Å
Source Organism:
Similar Structures:
Biological Unit for 1U04: monomeric; determined by author
Molecular Components in 1U04
Label Count Molecule
Protein (1 molecule)
1
Hypothetical Protein Pf0537
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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