3V0R: Crystal Structure Of Alternaria Alternata Allergen Alt A 1

Citation:
Abstract
BACKGROUND: Alternaria species is one of the most common molds associated with allergic diseases, and 80% of Alternaria species-sensitive patients produce IgE antibodies to a major protein allergen, Alt a 1. The structure and function of Alt a 1 is unknown. OBJECTIVE: We sought to obtain a high-resolution structure of Alt a 1 using x-ray crystallography and to investigate structural relationships between Alt a 1 and other allergens and proteins reported in the Protein Data Bank. METHODS: X-ray crystallography was used to determine the structure of Alt a 1 by using a custom-designed set of crystallization conditions. An initial Alt a 1 model was determined by the application of a Ta(6)Br(12)(2+) cluster and single-wavelength anomalous diffraction. Bioinformatic analyses were used to compare the Alt a 1 sequence and structure with that of other proteins. RESULTS: Alt a 1 is a unique beta-barrel comprising 11 beta-strands and forms a "butterfly-like" dimer linked by a single disulfide bond with a large (1345 A(2)) dimer interface. Intramolecular disulfide bonds are conserved among Alt a 1 homologs. Currently, the Alt a 1 structure has no equivalent in the Protein Data Bank. Bioinformatics analyses suggest that the structure is found exclusively in fungi. Four previously reported putative IgE-binding peptides have been located on the Alt a 1 structure. CONCLUSIONS: Alt a 1 has a unique, dimeric beta-barrel structure that appears to define a new protein family with unknown function found exclusively in fungi. The location of IgE antibody-binding epitopes is in agreement with the structural analysis of Alt a 1. The Alt a 1 structure will allow mechanistic structure/function studies and immunologic studies directed toward new forms of immunotherapy for Alternaria species-sensitive allergic patients.
PDB ID: 3V0RDownload
MMDB ID: 100459
PDB Deposition Date: 2011/12/8
Updated in MMDB: 2013/06
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Similar Structures:
Biological Unit for 3V0R: dimeric; determined by author and by software (PISA)
Molecular Components in 3V0R
Label Count Molecule
Proteins (2 molecules)
2
Major Allergen ALT a 1
Molecule annotation
Chemicals (16 molecules)
1
6
2
2
Molecule information is not avaliable.
3
6
4
2
* Click molecule labels to explore molecular sequence information.

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