|
Name |
Accession |
Description |
Interval |
E-value |
| hsFATP2a_ACSVL_like |
cd05938 |
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ... |
73-609 |
0e+00 |
|
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341261 [Multi-domain] Cd Length: 537 Bit Score: 997.57 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 73 FEKQVYTYLEVDKRSNQVARALQREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAK 152
Cdd:cd05938 1 FEGETYTYRDVDRRSNQAARALLAHAGLRPGDTVALLLGNEPAFLWIWLGLAKLGCPVAFLNTNIRSKSLLHCFRCCGAK 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 153 VLIAGADLKGAVEEVLPNLRENNVTIFYLNDEPITQGVESLKEKIEASSDQPIPKSFRITTTTKTAALYIYTSGTTGLPK 232
Cdd:cd05938 81 VLVVAPELQEAVEEVLPALRADGVSVWYLSHTSNTEGVISLLDKVDAASDEPVPASLRAHVTIKSPALYIYTSGTTGLPK 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 233 AAIINQRRLWGASFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWNDCREYNTTAIQYIG 312
Cdd:cd05938 161 AARISHLRVLQCSGFLSLCGVTADDVIYITLPLYHSSGFLLGIGGCIELGATCVLKPKFSASQFWDDCRKHNVTVIQYIG 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 313 EMLRYLCSTPRKDNDRDHKVRIAVGNGLRADVWKEFLNRFGKIRICEFYAATEGNIAFINYSGKIGAVGRANFLHKRGVP 392
Cdd:cd05938 241 ELLRYLCNQPQSPNDRDHKVRLAIGNGLRADVWREFLRRFGPIRIREFYGSTEGNIGFFNYTGKIGAVGRVSYLYKLLFP 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 393 YELIKFNTEKEEPVRDANGHCIHVSKGETGLLVAKITTRSPFCGYAGSKTQSEKKILRDVFRKGDIFFNSGDLLMIDHDN 472
Cdd:cd05938 321 FELIKFDVEKEEPVRDAQGFCIPVAKGEPGLLVAKITQQSPFLGYAGDKEQTEKKLLRDVFKKGDVYFNTGDLLVQDQQN 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 473 FIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHEGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNY 552
Cdd:cd05938 401 FLYFHDRVGDTFRWKGENVATTEVADVLGLLDFLQEVNVYGVTVPGHEGRIGMAAVKLKPGHEFDGKKLYQHVREYLPAY 480
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 556957071 553 ARPRFVRIQEAIEITGTFKQRKVKLVEEGFDPAVIKDPLYFLDENEKTYVPMSLHIF 609
Cdd:cd05938 481 ARPRFLRIQDSLEITGTFKQQKVRLVEEGFNPSIISDPLYFLDETQKTYVPLTPDIY 537
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
53-619 |
0e+00 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 713.57 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 53 TLLDKFLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQREAaLKEGDTAALFLGNEPAYIWIWLGLAKLGCSMAC 132
Cdd:PRK08279 38 SLGDVFEEAAARHPDRPALLFEDQSISYAELNARANRYAHWAAARG-VGKGDVVALLMENRPEYLAAWLGLAKLGAVVAL 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 133 LNCNIRSRAFLHSFNCSGAKVLIAGADLKGAVEEVLPNL-RENNVTIFYLNDEPITQGVESLKEKIEASSDQPipKSFRI 211
Cdd:PRK08279 117 LNTQQRGAVLAHSLNLVDAKHLIVGEELVEAFEEARADLaRPPRLWVAGGDTLDDPEGYEDLAAAAAGAPTTN--PASRS 194
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 212 TTTTKTAALYIYTSGTTGLPKAAIINQRR--LWGASFLLSIsGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRR 289
Cdd:PRK08279 195 GVTAKDTAFYIYTSGTTGLPKAAVMSHMRwlKAMGGFGGLL-RLTPDDVLYCCLPLYHNTGGTVAWSSVLAAGATLALRR 273
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 290 KFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNGLRADVWKEFLNRFGKIRICEFYAATEGNIA 369
Cdd:PRK08279 274 KFSASRFWDDVRRYRATAFQYIGELCRYLLNQPPKPTDRDHRLRLMIGNGLRPDIWDEFQQRFGIPRILEFYAASEGNVG 353
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 370 FINYSGKIGAVGRANFLHKRgvPYELIKFNTEKEEPVRDANGHCIHVSKGETGLLVAKITTRSPFCGYAgSKTQSEKKIL 449
Cdd:PRK08279 354 FINVFNFDGTVGRVPLWLAH--PYAIVKYDVDTGEPVRDADGRCIKVKPGEVGLLIGRITDRGPFDGYT-DPEASEKKIL 430
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 450 RDVFRKGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHEGRVGMAAIK 529
Cdd:PRK08279 431 RDVFKKGDAWFNTGDLMRDDGFGHAQFVDRLGDTFRWKGENVATTEVENALSGFPGVEEAVVYGVEVPGTDGRAGMAAIV 510
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 530 LKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKLVEEGFDPAVIKDPLYFLDENEKTYVPMSLHIF 609
Cdd:PRK08279 511 LADGAEFDLAALAAHLYERLPAYAVPLFVRLVPELETTGTFKYRKVDLRKEGFDPSKVDDPLYVLDPGSGGYVPLTAELY 590
|
570
....*....|
gi 556957071 610 ESIINKKFKL 619
Cdd:PRK08279 591 AEIAAGKFRL 600
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
76-584 |
0e+00 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 643.64 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 76 QVYTYLEVDKRSNQVARALQREAaLKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKVLI 155
Cdd:cd05940 2 EALTYAELDAMANRYARWLKSLG-LKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLAHCLNVSSAKHLV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 156 AgadlkgaveevlpnlrennvtifylndepitqgveslkekieassdqpipksfrittttkTAALYIYTSGTTGLPKAAI 235
Cdd:cd05940 81 V------------------------------------------------------------DAALYIYTSGTTGLPKAAI 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 236 INQRRLWGASFLLSISGVT-SEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWNDCREYNTTAIQYIGEM 314
Cdd:cd05940 101 ISHRRAWRGGAFFAGSGGAlPSDVLYTCLPLYHSTALIVGWSACLASGATLVIRKKFSASNFWDDIRKYQATIFQYIGEL 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 315 LRYLCSTPRKDNDRDHKVRIAVGNGLRADVWKEFLNRFGKIRICEFYAATEGNIAFINYSGKIGAVGRANFLHKRGVPYE 394
Cdd:cd05940 181 CRYLLNQPPKPTERKHKVRMIFGNGLRPDIWEEFKERFGVPRIAEFYAATEGNSGFINFFGKPGAIGRNPSLLRKVAPLA 260
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 395 LIKFNTEKEEPVRDANGHCIHVSKGETGLLVAKITTRSPFCGYAGSKTqSEKKILRDVFRKGDIFFNSGDLLMIDHDNFI 474
Cdd:cd05940 261 LVKYDLESGEPIRDAEGRCIKVPRGEPGLLISRINPLEPFDGYTDPAA-TEKKILRDVFKKGDAWFNTGDLMRLDGEGFW 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 475 YFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHEGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYAR 554
Cdd:cd05940 340 YFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVYGVQVPGTDGRAGMAAIVLQPNEEFDLSALAAHLEKNLPGYAR 419
|
490 500 510
....*....|....*....|....*....|
gi 556957071 555 PRFVRIQEAIEITGTFKQRKVKLVEEGFDP 584
Cdd:cd05940 420 PLFLRLQPEMEITGTFKQQKVDLRNEGFDP 449
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
78-584 |
1.50e-172 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 498.87 E-value: 1.50e-172
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 78 YTYLEVDKRSNQVARALQREAaLKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKVLIag 157
Cdd:cd05939 4 WTFRELNEYSNKVANFFQAQG-YRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHCITVSKAKALI-- 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 158 adlkgaveevlpnlrennvtiFYLNDEpitqgvesLKEKIEASSDQPIPKSFRittttkTAALYIYTSGTTGLPKAAII- 236
Cdd:cd05939 81 ---------------------FNLLDP--------LLTQSSTEPPSQDDVNFR------DKLFYIYTSGTTGLPKAAVIv 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 237 NQRRLWGASFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWNDCREYNTTAIQYIGEMLR 316
Cdd:cd05939 126 HSRYYRIAAGAYYAFGMRPEDVVYDCLPLYHSAGGIMGVGQALLHGSTVVIRKKFSASNFWDDCVKYNCTIVQYIGEICR 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 317 YLCSTPRKDNDRDHKVRIAVGNGLRADVWKEFLNRFGKIRICEFYAATEGNIAFINYSGKIGAVGRANFLHKRGVPYELI 396
Cdd:cd05939 206 YLLAQPPSEEEQKHNVRLAVGNGLRPQIWEQFVRRFGIPQIGEFYGATEGNSSLVNIDNHVGACGFNSRILPSVYPIRLI 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 397 KFNTEKEEPVRDANGHCIHVSKGETGLLVAKITTRSP---FCGYAgSKTQSEKKILRDVFRKGDIFFNSGDLLMIDHDNF 473
Cdd:cd05939 286 KVDEDTGELIRDSDGLCIPCQPGEPGLLVGKIIQNDPlrrFDGYV-NEGATNKKIARDVFKKGDSAFLSGDVLVMDELGY 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 474 IYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHEGRVGMAAIKLKEGKKFdgPNIYKHVME-YLPNY 552
Cdd:cd05939 365 LYFKDRTGDTFRWKGENVSTTEVEGILSNVLGLEDVVVYGVEVPGVEGRAGMAAIVDPERKVD--LDRFSAVLAkSLPPY 442
|
490 500 510
....*....|....*....|....*....|..
gi 556957071 553 ARPRFVRIQEAIEITGTFKQRKVKLVEEGFDP 584
Cdd:cd05939 443 ARPQFIRLLPEVDKTGTFKLQKTDLQKEGYDP 474
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
73-584 |
6.78e-150 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 441.10 E-value: 6.78e-150
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 73 FEKQVYTYLEVDKRSNQVARALQREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAK 152
Cdd:cd05937 1 FEGKTWTYSETYDLVLRYAHWLHDDLGVQAGDFVAIDLTNSPEFVFLWLGLWSIGAAPAFINYNLSGDPLIHCLKLSGSR 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 153 VLIAGADlkgaveevlpnlrennvtifylndepitqgveslkekieassdqpipksfrittttkTAALYIYTSGTTGLPK 232
Cdd:cd05937 81 FVIVDPD---------------------------------------------------------DPAILIYTSGTTGLPK 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 233 AAIINQRRLW-GASFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWNDCREYNTTAIQYI 311
Cdd:cd05937 104 AAAISWRRTLvTSNLLSHDLNLKNGDRTYTCMPLYHGTAAFLGACNCLMSGGTLALSRKFSASQFWKDVRDSGATIIQYV 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 312 GEMLRYLCSTPRKDNDRDHKVRIAVGNGLRADVWKEFLNRFGKIRICEFYAATEGNIAFINYSG---KIGAVGRANFLHK 388
Cdd:cd05937 184 GELCRYLLSTPPSPYDRDHKVRVAWGNGLRPDIWERFRERFNVPEIGEFYAATEGVFALTNHNVgdfGAGAIGHHGLIRR 263
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 389 R--GVPYELIKFNTEKEEPVRD-ANGHCIHVSKGETGLLVAKIT--TRSPFCGYAGSKTQSEKKILRDVFRKGDIFFNSG 463
Cdd:cd05937 264 WkfENQVVLVKMDPETDDPIRDpKTGFCVRAPVGEPGEMLGRVPfkNREAFQGYLHNEDATESKLVRDVFRKGDIYFRTG 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 464 DLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHEGRVGMAAIKLKEGKKFDGPNIY- 542
Cdd:cd05937 344 DLLRQDADGRWYFLDRLGDTFRWKSENVSTTEVADVLGAHPDIAEANVYGVKVPGHDGRAGCAAITLEESSAVPTEFTKs 423
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 556957071 543 ---KHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKLVEEGFDP 584
Cdd:cd05937 424 llaSLARKNLPSYAVPLFLRLTEEVATTDNHKQQKGVLRDEGVDP 468
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
78-577 |
8.15e-84 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 268.78 E-value: 8.15e-84
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 78 YTYLEVDKRSNQVARALQrEAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKVLIag 157
Cdd:cd05934 4 WTYAELLRESARIAAALA-ALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVV-- 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 158 adlkgaveevlpnlrennVTIFYLndepitqgveslkekieassdqpipksfrittttktaalyIYTSGTTGLPKAAIIN 237
Cdd:cd05934 81 ------------------VDPASI----------------------------------------LYTSGTTGPPKGVVIT 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 238 QRRL-WGASFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWNDCREYNTTAIQYIGEMLR 316
Cdd:cd05934 103 HANLtFAGYYSARRFGLGEDDVYLTVLPLFHINAQAVSVLAALSVGATLVLLPRFSASRFWSDVRRYGATVTNYLGAMLS 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 317 YLCSTPRKDNDRDHKVRIAVGNGLRADVWKEFLNRFGkIRICEFYAATEGNIAFIN---YSGKIGAVGRANFlhkrgvpy 393
Cdd:cd05934 183 YLLAQPPSPDDRAHRLRAAYGAPNPPELHEEFEERFG-VRLLEGYGMTETIVGVIGprdEPRRPGSIGRPAP-------- 253
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 394 elikfntEKEEPVRDANGHciHVSKGETGLLVakITTRSP---FCGYAGSKTQSEKkilrdVFRKGdiFFNSGDLLMIDH 470
Cdd:cd05934 254 -------GYEVRIVDDDGQ--ELPAGEPGELV--IRGLRGwgfFKGYYNMPEATAE-----AMRNG--WFHTGDLGYRDA 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 471 DNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHEGRVgMAAIKLKEGKKFDGPNIYKHVMEYLP 550
Cdd:cd05934 316 DGFFYFVDRKKDMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGEDEV-KAVVVLRPGETLDPEELFAFCEGQLA 394
|
490 500
....*....|....*....|....*..
gi 556957071 551 NYARPRFVRIQEAIEITGTFKQRKVKL 577
Cdd:cd05934 395 YFKVPRYIRFVDDLPKTPTEKVAKAQL 421
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
54-580 |
1.89e-83 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 268.99 E-value: 1.89e-83
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 54 LLDKFLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQrEAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACL 133
Cdd:COG0318 1 LADLLRRAAARHPDRPALVFGGRRLTYAELDARARRLAAALR-ALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPL 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 134 NCNIRSRAFLHSFNCSGAKVLIAgadlkgaveevlpnlrennvtifylndepitqgveslkekieassdqpipksfritt 213
Cdd:COG0318 80 NPRLTAEELAYILEDSGARALVT--------------------------------------------------------- 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 214 ttktaALYIYTSGTTGLPKAAIINQRRLW-GASFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFS 292
Cdd:COG0318 103 -----ALILYTSGTTGRPKGVMLTHRNLLaNAAAIAAALGLTPGDVVLVALPLFHVFGLTVGLLAPLLAGATLVLLPRFD 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 293 ASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAV--GNGLRADVWKEFLNRFGkIRICEFYAATEGN-IA 369
Cdd:COG0318 178 PERVLELIERERVTVLFGVPTMLARLLRHPEFARYDLSSLRLVVsgGAPLPPELLERFEERFG-VRIVEGYGLTETSpVV 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 370 FINYSG----KIGAVGRAnflhkrgVPYELIKfntekeepVRDANGHciHVSKGETGLLVAkittRSP--FCGYAGS--K 441
Cdd:COG0318 257 TVNPEDpgerRPGSVGRP-------LPGVEVR--------IVDEDGR--ELPPGEVGEIVV----RGPnvMKGYWNDpeA 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 442 TqsekkilRDVFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHeG 521
Cdd:COG0318 316 T-------AEAFRDG--WLRTGDLGRLDEDGYLYIVGRKKDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKW-G 385
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 556957071 522 RVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKLVEE 580
Cdd:COG0318 386 ERVVAFVVLRPGAELDAEELRAFLRERLARYKVPRRVEFVDELPRTASGKIDRRALRER 444
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
60-585 |
1.44e-73 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 245.82 E-value: 1.44e-73
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 60 QQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQrEAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRS 139
Cdd:PRK06155 29 RQAERYPDRPLLVFGGTRWTYAEAARAAAAAAHALA-AAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVPINTALRG 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 140 RAFLHSFNCSGAKVLIAGADLKGAVEEVLPnlRENNVTIFYLNDEPITQGVES---------LKEKIEASSDQPipksfr 210
Cdd:PRK06155 108 PQLEHILRNSGARLLVVEAALLAALEAADP--GDLPLPAVWLLDAPASVSVPAgwstaplppLDAPAPAAAVQP------ 179
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 211 itttTKTAALyIYTSGTTGLPKAAIINQRRL--WGAsFLLSISGVTSEDVVYTPLPLYHSAGL------LLgvNGCielg 282
Cdd:PRK06155 180 ----GDTAAI-LYTSGTTGPSKGVCCPHAQFywWGR-NSAEDLEIGADDVLYTTLPLFHTNALnaffqaLL--AGA---- 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 283 tTCVLRRKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNGLRADVWKEFLNRFGkIRICEFYA 362
Cdd:PRK06155 248 -TYVLEPRFSASGFWPAVRRHGATVTYLLGAMVSILLSQPARESDRAHRVRVALGPGVPAALHAAFRERFG-VDLLDGYG 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 363 ATEGN--IAFINYSGKIGAVGRAnflhKRGVpyelikfntekeePVRDANGHCIHVSKGETGLLVAKitTRSPFCGYAGS 440
Cdd:PRK06155 326 STETNfvIAVTHGSQRPGSMGRL----APGF-------------EARVVDEHDQELPDGEPGELLLR--ADEPFAFATGY 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 441 KTQSEKKIlrDVFRkgDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHE 520
Cdd:PRK06155 387 FGMPEKTV--EAWR--NLWFHTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVFPVPSELGE 462
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 556957071 521 GRVgMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKLVEEGFDPA 585
Cdd:PRK06155 463 DEV-MAAVVLRDGTALEPVALVRHCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVLREQGVTAD 526
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
67-594 |
2.39e-59 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 207.23 E-value: 2.39e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 67 HKPFLVFEKQVYTYLEVDKRSNQVARALqreAALKEGDTAA---LFLGNEPAYIwIWLGLAKL-GCSMACLNCNIRSRAF 142
Cdd:PRK07867 18 DDRGLYFEDSFTSWREHIRGSAARAAAL---RARLDPTRPPhvgVLLDNTPEFS-LLLGAAALsGIVPVGLNPTRRGAAL 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 143 LHSFNCSGAKVLIAGADLKGAVEEVLPNLRENNVTIFYLNDEpitqgveslkekIEASSDQPIPksfRITTTTKTAALYI 222
Cdd:PRK07867 94 ARDIAHADCQLVLTESAHAELLDGLDPGVRVINVDSPAWADE------------LAAHRDAEPP---FRVADPDDLFMLI 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 223 YTSGTTGLPKAAIINQRRLWGASFLLSIS-GVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWNDCR 301
Cdd:PRK07867 159 FTSGTSGDPKAVRCTHRKVASAGVMLAQRfGLGPDDVCYVSMPLFHSNAVMAGWAVALAAGASIALRRKFSASGFLPDVR 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 302 EYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGN-GLRADVwKEFLNRFGkIRICEFYAATEGNIAFINYSG-KIGA 379
Cdd:PRK07867 239 RYGATYANYVGKPLSYVLATPERPDDADNPLRIVYGNeGAPGDI-ARFARRFG-CVVVDGFGSTEGGVAITRTPDtPPGA 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 380 VGRAnflhKRGVpyELIKFNTEKEEP--VRDANGHCihVSKGETGLLVaKITTRSPFCGYAGSKTQSEKKIlrdvfRKGd 457
Cdd:PRK07867 317 LGPL----PPGV--AIVDPDTGTECPpaEDADGRLL--NADEAIGELV-NTAGPGGFEGYYNDPEADAERM-----RGG- 381
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 458 iFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHEGRVgMAAIKLKEGKKFD 537
Cdd:PRK07867 382 -VYWSGDLAYRDADGYAYFAGRLGDWMRVDGENLGTAPIERILLRYPDATEVAVYAVPDPVVGDQV-MAALVLAPGAKFD 459
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 556957071 538 GPNI--YKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKLVEEGFDPAvikDPLYFL 594
Cdd:PRK07867 460 PDAFaeFLAAQPDLGPKQWPSYVRVCAELPRTATFKVLKRQLSAEGVDCA---DPVWWI 515
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
219-571 |
1.00e-58 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 200.20 E-value: 1.00e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 219 ALYIYTSGTTGLPKAAIINQRRL-WGASFLLSISGVTSEDVVYTPLPLYHSAGLLlGVNGCIELGTTCVLRRKFSASQFW 297
Cdd:cd04433 3 ALILYTSGTTGKPKGVVLSHRNLlAAAAALAASGGLTEGDVFLSTLPLFHIGGLF-GLLGALLAGGTVVLLPKFDPEAAL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 298 NDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNG--LRADVWKEFLNRFGkIRICEFYAATEGNIAFI---- 371
Cdd:cd04433 82 ELIEREKVTILLGVPTLLARLLKAPESAGYDLSSLRALVSGGapLPPELLERFEEAPG-IKLVNGYGLTETGGTVAtgpp 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 372 -NYSGKIGAVGRAnflhkrgVPYELIKfntekeepVRDANGHciHVSKGETGLLVakITTRSPFCGYAGSKTQSEkkilr 450
Cdd:cd04433 161 dDDARKPGSVGRP-------VPGVEVR--------IVDPDGG--ELPPGEIGELV--VRGPSVMKGYWNNPEATA----- 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 451 dvFRKGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEhEGRVGMAAIKL 530
Cdd:cd04433 217 --AVDEDGWYRTGDLGRLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPE-WGERVVAVVVL 293
|
330 340 350 360
....*....|....*....|....*....|....*....|.
gi 556957071 531 KEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFK 571
Cdd:cd04433 294 RPGADLDAEELRAHVRERLAPYKVPRRVVFVDALPRTASGK 334
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
67-583 |
3.51e-50 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 182.53 E-value: 3.51e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 67 HKPFLVFEKQVYTYLEVdkrsnqVARALQREAALKE-GDTA-----ALFLGNEPAYIwIWLGLAKLGCSMAC-LNCNIRS 139
Cdd:PRK13388 16 DTIAVRYGDRTWTWREV------LAEAAARAAALIAlADPDrplhvGVLLGNTPEML-FWLAAAALGGYVLVgLNTTRRG 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 140 RAFLHSFNCSGAKVLI---AGADLKGAVEevLPNLRennvtiFYLNDEPitqgveSLKEKIEASSD-QPIPksfriTTTT 215
Cdd:PRK13388 89 AALAADIRRADCQLLVtdaEHRPLLDGLD--LPGVR------VLDVDTP------AYAELVAAAGAlTPHR-----EVDA 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 216 KTAALYIYTSGTTGLPKAAIINQRRL-WGASFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSAS 294
Cdd:PRK13388 150 MDPFMLIFTSGTTGAPKAVRCSHGRLaFAGRALTERFGLTRDDVCYVSMPLFHSNAVMAGWAPAVASGAAVALPAKFSAS 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 295 QFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNGLRADVWKEFLNRFGkIRICEFYAATEGNIAFINYS 374
Cdd:PRK13388 230 GFLDDVRRYGATYFNYVGKPLAYILATPERPDDADNPLRVAFGNEASPRDIAEFSRRFG-CQVEDGYGSSEGAVIVVREP 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 375 GK-IGAVGRanflhkrgvPYE-LIKFN--TEKEEPVR--DANGHCIHVSKGeTGLLVAKiTTRSPFCGYAGSKTQSEKKI 448
Cdd:PRK13388 309 GTpPGSIGR---------GAPgVAIYNpeTLTECAVArfDAHGALLNADEA-IGELVNT-AGAGFFEGYYNNPEATAERM 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 449 lrdvfRKGDifFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPehegRVG---M 525
Cdd:PRK13388 378 -----RHGM--YWSGDLAYRDADGWIYFAGRTADWMRVDGENLSAAPIERILLRHPAINRVAVYAVPDE----RVGdqvM 446
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 556957071 526 AAIKLKEGKKFDgPN---IYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKLVEEGFD 583
Cdd:PRK13388 447 AALVLRDGATFD-PDafaAFLAAQPDLGTKAWPRYVRIAADLPSTATNKVLKRELIAQGWA 506
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
60-574 |
5.53e-48 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 173.95 E-value: 5.53e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 60 QQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQREAaLKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRS 139
Cdd:cd17631 3 RRARRHPDRTALVFGGRSLTYAELDERVNRLAHALRALG-VAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLTP 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 140 RAFLHSFNCSGAKVLIagadlkgaveevlpnlrennvtifylnDEPitqgveslkekieassdqpipksfrittttktaA 219
Cdd:cd17631 82 PEVAYILADSGAKVLF---------------------------DDL---------------------------------A 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 220 LYIYTSGTTGLPKAAIINQR-RLWGASFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWN 298
Cdd:cd17631 102 LLMYTSGTTGRPKGAMLTHRnLLWNAVNALAALDLGPDDVLLVVAPLFHIGGLGVFTLPTLLRGGTVVILRKFDPETVLD 181
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 299 DCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNG--LRADVWKEFLNRfgKIRICEFYAATE--GNIAFI--- 371
Cdd:cd17631 182 LIERHRVTSFFLVPTMIQALLQHPRFATTDLSSLRAVIYGGapMPERLLRALQAR--GVKFVQGYGMTEtsPGVTFLspe 259
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 372 NYSGKIGAVGRANFLHkrgvpyelikfntekEEPVRDANGHciHVSKGETGllvaKITTRSP--FCGYAGsktQSEKKil 449
Cdd:cd17631 260 DHRRKLGSAGRPVFFV---------------EVRIVDPDGR--EVPPGEVG----EIVVRGPhvMAGYWN---RPEAT-- 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 450 RDVFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGvtVP-EHEGRVGMAAI 528
Cdd:cd17631 314 AAAFRDG--WFHTGDLGRLDEDGYLYIVDRKKDMIISGGENVYPAEVEDVLYEHPAVAEVAVIG--VPdEKWGEAVVAVV 389
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 556957071 529 KLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRK 574
Cdd:cd17631 390 VPRPGAELDEDELIAHCRERLARYKIPKSVEFVDALPRNATGKILK 435
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
58-487 |
1.06e-46 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 169.80 E-value: 1.06e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 58 FLQQVEKQPHKPFL-VFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGcsMACLNCN 136
Cdd:pfam00501 1 LERQAARTPDKTALeVGEGRRLTYRELDERANRLAAGL-RALGVGKGDRVAILLPNSPEWVVAFLACLKAG--AVYVPLN 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 137 IRSRAF--LHSFNCSGAKVLIAGADLKGAVEEVLPNLRENNVTIFYLNDEPITQGVESLKEKIEASSDQPIPKSFRITTT 214
Cdd:pfam00501 78 PRLPAEelAYILEDSGAKVLITDDALKLEELLEALGKLEVVKLVLVLDRDPVLKEEPLPEEAKPADVPPPPPPPPDPDDL 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 215 tktaALYIYTSGTTGLPKAAIINQR-----RLWGASFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRR 289
Cdd:pfam00501 158 ----AYIIYTSGTTGKPKGVMLTHRnlvanVLSIKRVRPRGFGLGPDDRVLSTLPLFHDFGLSLGLLGPLLAGATVVLPP 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 290 KFSA---SQFWNDCREYNTTAIQYIGEMLRYLCSTPR-KDNDRDH-KVRIAVGNGLRADVWKEFLNRFGkIRICEFYAAT 364
Cdd:pfam00501 234 GFPAldpAALLELIERYKVTVLYGVPTLLNMLLEAGApKRALLSSlRLVLSGGAPLPPELARRFRELFG-GALVNGYGLT 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 365 EGNIAFINYS------GKIGAVGRAnflhKRGVPYELIkfNTEKEEPVRDanghcihvskGETGLLVakitTRSP--FCG 436
Cdd:pfam00501 313 ETTGVVTTPLpldedlRSLGSVGRP----LPGTEVKIV--DDETGEPVPP----------GEPGELC----VRGPgvMKG 372
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|...
gi 556957071 437 YAG--SKTqsekkilRDVFRKGDiFFNSGDLLMIDHDNFIYFKDRIGDTFRWK 487
Cdd:pfam00501 373 YLNdpELT-------AEAFDEDG-WYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
53-575 |
1.28e-45 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 169.21 E-value: 1.28e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 53 TLLDKFLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMAC 132
Cdd:PRK06187 7 TIGRILRHGARKHPDKEAVYFDGRRTTYAELDERVNRLANAL-RALGVKKGDRVAVFDWNSHEYLEAYFAVPKIGAVLHP 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 133 LncNIRSRA--FLHSFNCSGAKVLIAGADLKGAVEEVLPNLreNNV-TIFYLNDEPITQG---VESLKEKIEASSDQPIP 206
Cdd:PRK06187 86 I--NIRLKPeeIAYILNDAEDRVVLVDSEFVPLLAAILPQL--PTVrTVIVEGDGPAAPLapeVGEYEELLAAASDTFDF 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 207 KSFRIttttKTAALYIYTSGTTGLPKAAIINQRRLwgasFLLSIS-----GVTSEDVVYTPLPLYHSAGLLLGVNGcIEL 281
Cdd:PRK06187 162 PDIDE----NDAAAMLYTSGTTGHPKGVVLSHRNL----FLHSLAvcawlKLSRDDVYLVIVPMFHVHAWGLPYLA-LMA 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 282 GTTCVLRRKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRkDNDRD-HKVRIAV--GNGLRADVWKEFLNRFGkIRIC 358
Cdd:PRK06187 233 GAKQVIPRRFDPENLLDLIETERVTFFFAVPTIWQMLLKAPR-AYFVDfSSLRLVIygGAALPPALLREFKEKFG-IDLV 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 359 EFYAATE-GNIAFINY--------SGKIGAVGRANFLHkrgvpyelikfntekEEPVRDANGHCIHVSKGETGLLVakit 429
Cdd:PRK06187 311 QGYGMTEtSPVVSVLPpedqlpgqWTKRRSAGRPLPGV---------------EARIVDDDGDELPPDGGEVGEII---- 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 430 TRSPfC---GYAGSKTQSEKKIlrdvfRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFI 506
Cdd:PRK06187 372 VRGP-WlmqGYWNRPEATAETI-----DGG--WLHTGDVGYIDEDGYLYITDRIKDVIISGGENIYPRELEDALYGHPAV 443
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 556957071 507 QEASVYGvtVP-EHEGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFK-QRKV 575
Cdd:PRK06187 444 AEVAVIG--VPdEKWGERPVAVVVLKPGATLDAKELRAFLRGRLAKFKLPKRIAFVDELPRTSVGKiLKRV 512
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
54-578 |
1.49e-45 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 167.74 E-value: 1.49e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 54 LLDKFLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQrEAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACL 133
Cdd:cd05936 1 LADLLEEAARRFPDKTALIFMGRKLTYRELDALAEAFAAGLQ-NLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVVVPL 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 134 NCNIRSRAFLHSFNCSGAKVLIAGADLKGAVEevlpnlrennvtifylndepitqgveslkekieassdQPIPKSFRITT 213
Cdd:cd05936 80 NPLYTPRELEHILNDSGAKALIVAVSFTDLLA-------------------------------------AGAPLGERVAL 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 214 TTKTAALYIYTSGTTGLPKAAIINQRRLWgaSFLLSI-----SGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLR 288
Cdd:cd05936 123 TPEDVAVLQYTSGTTGVPKGAMLTHRNLV--ANALQIkawleDLLEGDDVVLAALPLFHVFGLTVALLLPLALGATIVLI 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 289 RKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNG--LRADVWKEFLNRFGKiRICEFYAATE- 365
Cdd:cd05936 201 PRFRPIGVLKEIRKHRVTIFPGVPTMYIALLNAPEFKKRDFSSLRLCISGGapLPVEVAERFEELTGV-PIVEGYGLTEt 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 366 -----GNiaFINYSGKIGAVGRAnflhkrgVPyelikfNTEKEepVRDANGHciHVSKGETGLLVAkittRSP--FCGYA 438
Cdd:cd05936 280 spvvaVN--PLDGPRKPGSIGIP-------LP------GTEVK--IVDDDGE--ELPPGEVGELWV----RGPqvMKGYW 336
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 439 GSKTQSEKkilrdVFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPE 518
Cdd:cd05936 337 NRPEETAE-----AFVDG--WLRTGDIGYMDEDGYFFIVDRKKDMIIVGGFNVYPREVEEVLYEHPAVAEAAVVGVPDPY 409
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 519 HeGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKLV 578
Cdd:cd05936 410 S-GEAVKAFVVLKEGASLTEEEIIAFCREQLAGYKVPRQVEFRDELPKSAVGKILRRELR 468
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
74-552 |
3.52e-38 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 147.36 E-value: 3.52e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 74 EKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKV 153
Cdd:cd05911 7 TGKELTYAQLRTLSRRLAAGL-RKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKV 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 154 LIAGAD----LKGAVEEVLPNLRennVTIFYLNDEPITQGVESLKEKIEAS-SDQPIPKsfrITTTTKTAALyIYTSGTT 228
Cdd:cd05911 86 IFTDPDglekVKEAAKELGPKDK---IIVLDDKPDGVLSIEDLLSPTLGEEdEDLPPPL---KDGKDDTAAI-LYSSGTT 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 229 GLPKAAIINQRRLWgASFLLSIS----GVTSEDVVYTPLPLYHSAGLLLGVNGCIeLGTTCVLRRKFSASQFWNDCREYN 304
Cdd:cd05911 159 GLPKGVCLSHRNLI-ANLSQVQTflygNDGSNDVILGFLPLYHIYGLFTTLASLL-NGATVIIMPKFDSELFLDLIEKYK 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 305 TTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNGlrADVWKEFLNRFGKIR----ICEFYAATE-GNIAFINYSG--KI 377
Cdd:cd05911 237 ITFLYLVPPIAAALAKSPLLDKYDLSSLRVILSGG--APLSKELQELLAKRFpnatIKQGYGMTEtGGILTVNPDGddKP 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 378 GAVGR--ANFLHKrgvpyeLIKFNTEKEEPVRdanghcihvSKGEtgLLVakittRSPFC--GY-----AGSKTQSEkki 448
Cdd:cd05911 315 GSVGRllPNVEAK------IVDDDGKDSLGPN---------EPGE--ICV-----RGPQVmkGYynnpeATKETFDE--- 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 449 lrdvfrkgDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVpEHEGRVGMAAI 528
Cdd:cd05911 370 --------DGWLHTGDIGYFDEDGYLYIVDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPD-EVSGELPRAYV 440
|
490 500
....*....|....*....|....
gi 556957071 529 KLKEGKKFDGPNIYKHVMEYLPNY 552
Cdd:cd05911 441 VRKPGEKLTEKEVKDYVAKKVASY 464
|
|
| PRK07868 |
PRK07868 |
acyl-CoA synthetase; Validated |
60-581 |
8.37e-38 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236121 [Multi-domain] Cd Length: 994 Bit Score: 150.25 E-value: 8.37e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 60 QQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQrEAALKEGDTAALFLGNEPAYIWIWLGLAKLGcsmaclncnirs 139
Cdd:PRK07868 455 EQARDAPKGEFLLFDGRVHTYEAVNRRINNVVRGLI-AVGVRQGDRVGVLMETRPSALVAIAALSRLG------------ 521
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 140 raflhsfncSGAKVLIAGADLK-----GAVEEVL---PNLRENNVT---IFYLN-----DEPITQGVESLK-EKIEASSD 202
Cdd:PRK07868 522 ---------AVAVLMPPDTDLAaavrlGGVTEIItdpTNLEAARQLpgrVLVLGggesrDLDLPDDADVIDmEKIDPDAV 592
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 203 QpIPKSFRITTTTKTAALYIYTSGTTG-LPKAAIINQRrlWGASFL--LSISGVTSEDVVYTPLPLYHSAGLLLGVNGCI 279
Cdd:PRK07868 593 E-LPGWYRPNPGLARDLAFIAFSTAGGeLVAKQITNYR--WALSAFgtASAAALDRRDTVYCLTPLHHESGLLVSLGGAV 669
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 280 ELGTTCVLRRKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNGLRADVWKEFLNRFGKIRICE 359
Cdd:PRK07868 670 VGGSRIALSRGLDPDRFVQEVRQYGVTVVSYTWAMLREVVDDPAFVLHGNHPVRLFIGSGMPTGLWERVVEAFAPAHVVE 749
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 360 FYAATEGNIAFINYSG-KIGAVGRAnfLHKRGvPYELIKFNTEKEEPVRDANGHCIHVSKGETGLLVAKitTRSPFCGYA 438
Cdd:PRK07868 750 FFATTDGQAVLANVSGaKIGSKGRP--LPGAG-RVELAAYDPEHDLILEDDRGFVRRAEVNEVGVLLAR--ARGPIDPTA 824
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 439 GsktqsekkILRDVFRKGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPE 518
Cdd:PRK07868 825 S--------VKRGVFAPADTWISTEYLFRRDDDGDYWLVDRRGSVIRTARGPVYTEPVTDALGRIGGVDLAVTYGVEVGG 896
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 556957071 519 HEgrVGMAAIKLKEGKKFDGPNIyKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKLVEEG 581
Cdd:PRK07868 897 RQ--LAVAAVTLRPGAAITAADL-TEALASLPVGLGPDIVHVVPEIPLSATYRPTVSALRAAG 956
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
66-558 |
2.09e-35 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 140.45 E-value: 2.09e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 66 PHKPFLVFEKQVYTYLEVDKRSNQVARALQReAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHS 145
Cdd:PRK07788 63 PDRAALIDERGTLTYAELDEQSNALARGLLA-LGVRAGDGVAVLARNHRGFVLALYAAGKVGARIILLNTGFSGPQLAEV 141
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 146 FNCSGAKVLIAGADLKGAVEEVLPNLRENNVTIFYL-NDEPITQGVESLKEKIEASSDQPIPKSfritttTKTAALYIYT 224
Cdd:PRK07788 142 AAREGVKALVYDDEFTDLLSALPPDLGRLRAWGGNPdDDEPSGSTDETLDDLIAGSSTAPLPKP------PKPGGIVILT 215
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 225 SGTTGLPKAAIINQrrlwgASFLLSISGVTS------EDVVYTPLPLYHSAGLLLGVNGCIeLGTTCVLRRKFSASQFWN 298
Cdd:PRK07788 216 SGTTGTPKGAPRPE-----PSPLAPLAGLLSrvpfraGETTLLPAPMFHATGWAHLTLAMA-LGSTVVLRRRFDPEATLE 289
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 299 DCREYNTTAIQYIGEMLRYLCSTPRK-DNDRDH---KVRIAVGNGLRADVWKEFLNRFGKIrICEFYAATEGNIAFI--- 371
Cdd:PRK07788 290 DIAKHKATALVVVPVMLSRILDLGPEvLAKYDTsslKIIFVSGSALSPELATRALEAFGPV-LYNLYGSTEVAFATIatp 368
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 372 -NYSGKIGAVGRAnflhKRGVPYELIkfntekeepvrDANGHCIHVskGETGllvaKITTRS--PFCGYAGSKTqseKKI 448
Cdd:PRK07788 369 eDLAEAPGTVGRP----PKGVTVKIL-----------DENGNEVPR--GVVG----RIFVGNgfPFEGYTDGRD---KQI 424
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 449 LrdvfrkgDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHEGRVGmAAI 528
Cdd:PRK07788 425 I-------DGLLSSGDVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDEEFGQRLR-AFV 496
|
490 500 510
....*....|....*....|....*....|
gi 556957071 529 KLKEGKKFDGPNIYKHVMEYLPNYARPRFV 558
Cdd:PRK07788 497 VKAPGAALDEDAIKDYVRDNLARYKVPRDV 526
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
62-577 |
1.99e-34 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 137.12 E-value: 1.99e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 62 VEKQPHKPFLVFEK-----QVYTYLEVDKRSNQVARaLQREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCN 136
Cdd:PRK08008 17 ADVYGHKTALIFESsggvvRRYSYLELNEEINRTAN-LFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINAR 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 137 IRSRAFLHSFNCSGAKVLIAGADLKGAVEEVLPNLRENNVTIFYLN-DEPITQGVESLKEkieASSDQPIPKSFRITTTT 215
Cdd:PRK08008 96 LLREESAWILQNSQASLLVTSAQFYPMYRQIQQEDATPLRHICLTRvALPADDGVSSFTQ---LKAQQPATLCYAPPLST 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 216 KTAALYIYTSGTTGLPKAAIINQRRLWGASFLLSISGVTSEDVVY-TPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSAS 294
Cdd:PRK08008 173 DDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYlTVMPAFHIDCQCTAAMAAFSAGATFVLLEKYSAR 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 295 QFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNGLRADVWKE-FLNRFGkIRICEFYAATEGNIafiny 373
Cdd:PRK08008 253 AFWGQVCKYRATITECIPMMIRTLMVQPPSANDRQHCLREVMFYLNLSDQEKDaFEERFG-VRLLTSYGMTETIV----- 326
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 374 sGKIG----------AVGRAnflhkrGVPYelikfntekEEPVRDANGHciHVSKGETG-LLVAKITTRSPFCGYAGsKT 442
Cdd:PRK08008 327 -GIIGdrpgdkrrwpSIGRP------GFCY---------EAEIRDDHNR--PLPAGEIGeICIKGVPGKTIFKEYYL-DP 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 443 QSEKKILRdvfrkGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHEGR 522
Cdd:PRK08008 388 KATAKVLE-----ADGWLHTGDTGYVDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIVVVGIKDSIRDEA 462
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 556957071 523 VgMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKL 577
Cdd:PRK08008 463 I-KAFVVLNEGETLSEEEFFAFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIKKNL 516
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
62-579 |
6.81e-32 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 129.59 E-value: 6.81e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 62 VEKQ----PHKPFLVFEKQVYTYLEVDKRSNQVARALQREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNI 137
Cdd:PRK06839 8 IEKRaylhPDRIAIITEEEEMTYKQLHEYVSKVAAYLIYELNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRL 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 138 RSRAFLHSFNCSGAKVLIAGADLKGAVEEVlpnlrennVTIFYLnDEPITqgVESLKEKIEASSDQPIPKSfrittttKT 217
Cdd:PRK06839 88 TENELIFQLKDSGTTVLFVEKTFQNMALSM--------QKVSYV-QRVIS--ITSLKEIEDRKIDNFVEKN-------ES 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 218 AALYI-YTSGTTGLPKAAIINQRRL-WGASFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQ 295
Cdd:PRK06839 150 ASFIIcYTSGTTGKPKGAVLTQENMfWNALNNTFAIDLTMHDRSIVLLPLFHIGGIGLFAFPTLFAGGVIIVPRKFEPTK 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 296 FWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNGLRA--DVWKEFLNRfgKIRICEFYAATEGN-----I 368
Cdd:PRK06839 230 ALSMIEKHKVTVVMGVPTIHQALINCSKFETTNLQSVRWFYNGGAPCpeELMREFIDR--GFLFGQGFGMTETSptvfmL 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 369 AFINYSGKIGAVGRANFLHKrgvpYELIKFNTEKEEPvrdanghcihvskGETGllvaKITTRSP--FCGYAGSKTQSEK 446
Cdd:PRK06839 308 SEEDARRKVGSIGKPVLFCD----YELIDENKNKVEV-------------GEVG----ELLIRGPnvMKEYWNRPDATEE 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 447 KIlrdvfRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHeGRVGMA 526
Cdd:PRK06839 367 TI-----QDG--WLCTGDLARVDEDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHVKW-GEIPIA 438
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 556957071 527 AIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKLVE 579
Cdd:PRK06839 439 FIVKKSSSVLIEKDVIEHCRLFLAKYKIPKEIVFLKELPKNATGKIQKAQLVN 491
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
62-563 |
5.94e-31 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 126.97 E-value: 5.94e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 62 VEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRA 141
Cdd:PRK08316 21 ARRYPDKTALVFGDRSWTYAELDAAVNRVAAAL-LDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFMLTGEE 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 142 FLHSFNCSGAKVLIAGADLKGAVEEVLPNLreNNVTIFYLNDEPITQGVESLKEKIEASSDQPIPKSFRITTTTKTAALy 221
Cdd:PRK08316 100 LAYILDHSGARAFLVDPALAPTAEAALALL--PVDTLILSLVLGGREAPGGWLDFADWAEAGSVAEPDVELADDDLAQI- 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 222 IYTSGTTGLPKAAIINQRRLWgASFLLSI--SGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWND 299
Cdd:PRK08316 177 LYTSGTESLPKGAMLTHRALI-AEYVSCIvaGDMSADDIPLHALPLYHCAQLDVFLGPYLYVGATNVILDAPDPELILRT 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 300 CREYNTTAI-----QYIGeMLRylcstpRKDNDRdHKVRiavgnGLR----------ADVWKEFLNRFGKIRICEFYAAT 364
Cdd:PRK08316 256 IEAERITSFfapptVWIS-LLR------HPDFDT-RDLS-----SLRkgyygasimpVEVLKELRERLPGLRFYNCYGQT 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 365 E-GNIAFI----NYSGKIGAVGRAnflhkrgvpyeliKFNTEKEepVRDANGHciHVSKGETGllvaKITTRSP--FCGY 437
Cdd:PRK08316 323 EiAPLATVlgpeEHLRRPGSAGRP-------------VLNVETR--VVDDDGN--DVAPGEVG----EIVHRSPqlMLGY 381
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 438 AGSKTQSEkkilrDVFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVtvp 517
Cdd:PRK08316 382 WDDPEKTA-----EAFRGG--WFHSGDLGVMDEEGYITVVDRKKDMIKTGGENVASREVEEALYTHPAVAEVAVIGL--- 451
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 556957071 518 EHEGRVG--MAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEA 563
Cdd:PRK08316 452 PDPKWIEavTAVVVPKAGATVTEDELIAHCRARLAGFKVPKRVIFVDE 499
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
77-579 |
6.30e-31 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 126.66 E-value: 6.30e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 77 VYTYLEVDKRSNQVARALQReAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKVLIA 156
Cdd:cd05926 14 ALTYADLAELVDDLARQLAA-LGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYLADLGSKLVLT 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 157 -----GADLKGAVEEVLPNLR-ENNVTIFYLNDEPITQGVESLKEKIEASSDQPIPKSfrittttktAALYIYTSGTTGL 230
Cdd:cd05926 93 pkgelGPASRAASKLGLAILElALDVGVLIRAPSAESLSNLLADKKNAKSEGVPLPDD---------LALILHTSGTTGR 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 231 PKAAIINQRRLwgasfLLSISGV------TSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWNDCREYN 304
Cdd:cd05926 164 PKGVPLTHRNL-----AASATNItntyklTPDDRTLVVMPLFHVHGLVASLLSTLAAGGSVVLPPRFSASTFWPDVRDYN 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 305 TTAIQYIGEMLRYLCSTP-RKDNDRDHKVRIA--VGNGLRADVWKEFLNRFGkIRICEFYAATEG------NiAFINYSG 375
Cdd:cd05926 239 ATWYTAVPTIHQILLNRPePNPESPPPKLRFIrsCSASLPPAVLEALEATFG-APVLEAYGMTEAahqmtsN-PLPPGPR 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 376 KIGAVGRAnflhkrgvpyelikFNTEkeepVRDANghcihvSKGETGLLVAK--ITTRSP--FCGYAGSktqseKKILRD 451
Cdd:cd05926 317 KPGSVGKP--------------VGVE----VRILD------EDGEILPPGVVgeICLRGPnvTRGYLNN-----PEANAE 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 452 VFRKGDiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGvtVP-EHEGRVGMAAIKL 530
Cdd:cd05926 368 AAFKDG-WFRTGDLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFG--VPdEKYGEEVAAAVVL 444
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 556957071 531 KEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKLVE 579
Cdd:cd05926 445 REGASVTEEELRAFCRKHLAAFKVPKKVYFVDELPKTATGKIQRRKVAE 493
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
53-564 |
3.05e-30 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 124.63 E-value: 3.05e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 53 TLLDKFLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQrEAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMAC 132
Cdd:PRK07656 6 TLPELLARAARRFGDKEAYVFGDQRLTYAELNARVRRAAAALA-ALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVP 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 133 LN--------CNIRSRaflhsfncSGAKVLIAGADLKGAVEEV---LPNLrENNVTIFYLNDEPITQGVESLKEKIEASS 201
Cdd:PRK07656 85 LNtrytadeaAYILAR--------GDAKALFVLGLFLGVDYSAttrLPAL-EHVVICETEEDDPHTEKMKTFTDFLAAGD 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 202 DqpiPKSFRITTTTKTAALyIYTSGTTGLPKAAIINQRRL------WGAsfllsISGVTSEDVVYTPLPLYHSAGLLLGV 275
Cdd:PRK07656 156 P---AERAPEVDPDDVADI-LFTSGTTGRPKGAMLTHRQLlsnaadWAE-----YLGLTEGDRYLAANPFFHVFGYKAGV 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 276 NGCIELGTTCVLRRKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNGlrADVWKEFLNRF--- 352
Cdd:PRK07656 227 NAPLMRGATILPLPVFDPDEVFRLIETERITVLPGPPTMYNSLLQHPDRSAEDLSSLRLAVTGA--ASMPVALLERFese 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 353 -GKIRICEFYAATEGN-IAFINYSGK-----IGAVGRAnflhkrgvpyelIKfNTEkeepVRDANGHCIHVSKGETG-LL 424
Cdd:PRK07656 305 lGVDIVLTGYGLSEASgVTTFNRLDDdrktvAGTIGTA------------IA-GVE----NKIVNELGEEVPVGEVGeLL 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 425 VakittRSP--FCGYAGSKTQSEKKIlrdvfrKGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLEN 502
Cdd:PRK07656 368 V-----RGPnvMKGYYDDPEATAAAI------DADGWLHTGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYE 436
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 556957071 503 VDFIQEASVYGVTVPEHeGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAI 564
Cdd:PRK07656 437 HPAVAEAAVIGVPDERL-GEVGKAYVVLKPGAELTEEELIAYCREHLAKYKVPRSIEFLDEL 497
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
79-571 |
4.06e-28 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 117.19 E-value: 4.06e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 79 TYLEVDKRSNQVARALQReAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKVLIAGA 158
Cdd:cd05935 3 TYLELLEVVKKLASFLSN-KGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVVGS 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 159 DLkgavEEVlpnlrennvtifylndepitqgveslkekieassdqpipksfrittttktaALYIYTSGTTGLPKAAIINQ 238
Cdd:cd05935 82 EL----DDL---------------------------------------------------ALIPYTSGTTGLPKGCMHTH 106
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 239 RRLWGASFLLSI-SGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWNDCREYNTTAIQYIGEMLRY 317
Cdd:cd05935 107 FSAAANALQSAVwTGLTPSDVILACLPLFHVTGFVGSLNTAVYVGGTYVLMARWDRETALELIEKYKVTFWTNIPTMLVD 186
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 318 LCSTPRkDNDRDHKVRIAVGNG---LRADVWKEFLNRFGkIRICEFYAATEG-NIAFINYSG--KIGAVGRANFlhkrGV 391
Cdd:cd05935 187 LLATPE-FKTRDLSSLKVLTGGgapMPPAVAEKLLKLTG-LRFVEGYGLTETmSQTHTNPPLrpKLQCLGIP*F----GV 260
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 392 PYELIKFNTEKEEPVrdanghcihvskGETGllvaKITTRSP--FCGYAGSKTQSEKKILRDvfrKGDIFFNSGDLLMID 469
Cdd:cd05935 261 DARVIDIETGRELPP------------NEVG----EIVVRGPqiFKGYWNRPEETEESFIEI---KGRRFFRTGDLGYMD 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 470 HDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVygVTVP-EHEGRVGMAAIKLKEGK--KFDGPNIYKHVM 546
Cdd:cd05935 322 EEGYFFFVDRVKRMINVSGFKVWPAEVEAKLYKHPAI*EVCV--ISVPdERVGEEVKAFIVLRPEYrgKVTEEDIIEWAR 399
|
490 500
....*....|....*....|....*
gi 556957071 547 EYLPNYARPRFVRIQEAIEITGTFK 571
Cdd:cd05935 400 EQMAAYKYPREVEFVDELPRSASGK 424
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
60-514 |
4.35e-28 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 118.26 E-value: 4.35e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 60 QQVEKQPHKPFLVFEK--QVYTYLEVDKRSNQVARaLQREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNI 137
Cdd:PRK13391 5 IHAQTTPDKPAVIMAStgEVVTYRELDERSNRLAH-LFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHL 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 138 RSRAFLHSFNCSGAKVLI---AGADLKGAVEEVLPNLRENNVtifyLNDEPITQGVESLKEKIEASSDQPIPKSfrittT 214
Cdd:PRK13391 84 TPAEAAYIVDDSGARALItsaAKLDVARALLKQCPGVRHRLV----LDGDGELEGFVGYAEAVAGLPATPIADE-----S 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 215 TKTAALyiYTSGTTGLPKA--------AIINQRRLwgASFLLSISGVTSEDVVYTPLPLYHSAGLLLgVNGCIELGTTCV 286
Cdd:PRK13391 155 LGTDML--YSSGTTGRPKGikrplpeqPPDTPLPL--TAFLQRLWGFRSDMVYLSPAPLYHSAPQRA-VMLVIRLGGTVI 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 287 LRRKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDR-DH---KVRIAVGNGLRADVWKEFLNRFGKIrICEFYA 362
Cdd:PRK13391 230 VMEHFDAEQYLALIEEYGVTHTQLVPTMFSRMLKLPEEVRDKyDLsslEVAIHAAAPCPPQVKEQMIDWWGPI-IHEYYA 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 363 ATEGNIA-FIN---YSGKIGAVGRANFlhkrGVPYelikfntekeepVRDANGHciHVSKGETGLLVAKitTRSPFcGYA 438
Cdd:PRK13391 309 ATEGLGFtACDseeWLAHPGTVGRAMF----GDLH------------ILDDDGA--ELPPGEPGTIWFE--GGRPF-EYL 367
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 556957071 439 G--SKTQSEKKILRDVFRKGDIFFnsgdllmIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGV 514
Cdd:PRK13391 368 NdpAKTAEARHPDGTWSTVGDIGY-------VDEDGYLYLTDRAAFMIISGGVNIYPQEAENLLITHPKVADAAVFGV 438
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
54-564 |
7.05e-28 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 118.29 E-value: 7.05e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 54 LLDKflqQVEKQPHKPFLVFE-----KQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGC 128
Cdd:COG0365 14 CLDR---HAEGRGDKVALIWEgedgeERTLTYAELRREVNRFANAL-RALGVKKGDRVAIYLPNIPEAVIAMLACARIGA 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 129 SMACLNCNIRSRAFLHSFNCSGAKVLIAGA---------DLKGAVEEVLPNLR--ENNVTIFYLNDEPITQGVESLKEKI 197
Cdd:COG0365 90 VHSPVFPGFGAEALADRIEDAEAKVLITADgglrggkviDLKEKVDEALEELPslEHVIVVGRTGADVPMEGDLDWDELL 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 198 EASSDQPipksfritTTTKTAA---LYI-YTSGTTGLPKAAIINQR--RLWGASFLLSISGVTSEDVVYTPLPLY----H 267
Cdd:COG0365 170 AAASAEF--------EPEPTDAddpLFIlYTSGTTGKPKGVVHTHGgyLVHAATTAKYVLDLKPGDVFWCTADIGwatgH 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 268 S----AGLLLGVngcielgTTCVLRRKF---SASQFWNDCREYN-T------TAIQyigeMLRYLCSTPRKDNDRDHkVR 333
Cdd:COG0365 242 SyivyGPLLNGA-------TVVLYEGRPdfpDPGRLWELIEKYGvTvfftapTAIR----ALMKAGDEPLKKYDLSS-LR 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 334 IAVGNG--LRADVWKEFLNRFGkIRICEFYAATEGNIAFINYSG----KIGAVGRAnflhkrgVP-YELIkfntekeepV 406
Cdd:COG0365 310 LLGSAGepLNPEVWEWWYEAVG-VPIVDGWGQTETGGIFISNLPglpvKPGSMGKP-------VPgYDVA---------V 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 407 RDANGHciHVSKGETGLLVAKITTRSPFCGYAG--SKTqseKKILRDVFrKGdiFFNSGDLLMIDHDNFIYFKDRIGDTF 484
Cdd:COG0365 373 VDEDGN--PVPPGEEGELVIKGPWPGMFRGYWNdpERY---RETYFGRF-PG--WYRTGDGARRDEDGYFWILGRSDDVI 444
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 485 RWKGENVATCEVADLLENVDFIQEASVYGVtvpEHEGR-VGMAA-IKLKEGKKFDGP---NIYKHVMEYLPNYARPRFVR 559
Cdd:COG0365 445 NVSGHRIGTAEIESALVSHPAVAEAAVVGV---PDEIRgQVVKAfVVLKPGVEPSDElakELQAHVREELGPYAYPREIE 521
|
....*
gi 556957071 560 IQEAI 564
Cdd:COG0365 522 FVDEL 526
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
71-383 |
2.85e-27 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 115.77 E-value: 2.85e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 71 LVFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSG 150
Cdd:PRK08276 5 MAPSGEVVTYGELEARSNRLAHGL-RALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSG 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 151 AKVLIAGADLKGAVEEVLPNLrENNVTIFYLNDEPITqGVESLKEKIEASSDQPIPKSFRittttktAALYIYTSGTTGL 230
Cdd:PRK08276 84 AKVLIVSAALADTAAELAAEL-PAGVPLLLVVAGPVP-GFRSYEEALAAQPDTPIADETA-------GADMLYSSGTTGR 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 231 PKAAiinQRRLWGA---------SFLLSISGVTSEDVVY-TPLPLYHSAGLLLGVNgCIELGTTCVLRRKFSASQFWNDC 300
Cdd:PRK08276 155 PKGI---KRPLPGLdpdeapgmmLALLGFGMYGGPDSVYlSPAPLYHTAPLRFGMS-ALALGGTVVVMEKFDAEEALALI 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 301 REYNTTAIQYIGEMLRYLCSTPRKDNDR----DHKVRIAVGNGLRADVWKEFLNRFGKIrICEFYAATEGN-IAFIN--- 372
Cdd:PRK08276 231 ERYRVTHSQLVPTMFVRMLKLPEEVRARydvsSLRVAIHAAAPCPVEVKRAMIDWWGPI-IHEYYASSEGGgVTVITsed 309
|
330
....*....|.
gi 556957071 373 YSGKIGAVGRA 383
Cdd:PRK08276 310 WLAHPGSVGKA 320
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
220-556 |
4.20e-25 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 106.34 E-value: 4.20e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 220 LYI-YTSGTTGLPKAAIINQRRlWGASF-----LLSISGvtsEDVVYTPLPLYHSagllLGVNGCIE---LGTTCVLRRK 290
Cdd:cd17633 3 FYIgFTSGTTGLPKAYYRSERS-WIESFvcnedLFNISG---EDAILAPGPLSHS----LFLYGAISalyLGGTFIGQRK 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 291 FSASQFWNDCREYNTTAIQYIGEMLRYLCSTprkdNDRDHKVRI--AVGNGLRADVWKEFLNRFGKIRICEFYAATEGNi 368
Cdd:cd17633 75 FNPKSWIRKINQYNATVIYLVPTMLQALART----LEPESKIKSifSSGQKLFESTKKKLKNIFPKANLIEFYGTSELS- 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 369 aFINYSgkigAVGRANFLHKRGVPYElikfNTEKEepVRDANGhcihvskGETGllvaKITTRSP--FCGYagsktqsek 446
Cdd:cd17633 150 -FITYN----FNQESRPPNSVGRPFP----NVEIE--IRNADG-------GEIG----KIFVKSEmvFSGY--------- 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 447 kiLRDVFRKGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVtvpEHEGRvGMA 526
Cdd:cd17633 199 --VRGGFSNPDGWMSVGDIGYVDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGI---PDARF-GEI 272
|
330 340 350
....*....|....*....|....*....|
gi 556957071 527 AIKLKEGKKFDGPNIYKHVMEYLPNYARPR 556
Cdd:cd17633 273 AVALYSGDKLTYKQLKRFLKQKLSRYEIPK 302
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
53-571 |
4.37e-25 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 109.10 E-value: 4.37e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 53 TLLDKFLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQREAalKEGDTAALFLGNEPAYIWIWLGLAKLGCSmaC 132
Cdd:PRK07638 2 GITKEYKKHASLQPNKIAIKENDRVLTYKDWFESVCKVANWLNEKE--SKNKTIAILLENRIEFLQLFAGAAMAGWT--C 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 133 LNCNIRSRAflhsfNCSGAKVLIAGADLkgaveevLPNLRennvtiFYLNDEPITQG----VESLKEKIEASSDQPIPks 208
Cdd:PRK07638 78 VPLDIKWKQ-----DELKERLAISNADM-------IVTER------YKLNDLPDEEGrvieIDEWKRMIEKYLPTYAP-- 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 209 friTTTTKTAALYI-YTSGTTGLPKAAIINQRRlWGASFLLSIS--GVTSEDVVYTPLPLYHSAgLLLGVNGCIELGTTC 285
Cdd:PRK07638 138 ---IENVQNAPFYMgFTSGSTGKPKAFLRAQQS-WLHSFDCNVHdfHMKREDSVLIAGTLVHSL-FLYGAISTLYVGQTV 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 286 VLRRKFSASQFWNDCREYNTTAIQYIGEMLRYLCstpRKDNDRDHKVRIAV-GNGLRADVWKEFLNRFGKIRICEFYAAT 364
Cdd:PRK07638 213 HLMRKFIPNQVLDKLETENISVMYTVPTMLESLY---KENRVIENKMKIISsGAKWEAEAKEKIKNIFPYAKLYEFYGAS 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 365 EgnIAFINYSG------KIGAVGRanflhkrgvPYELIKfntekeepVRDANGHCIHVSKGETGllvaKITTRSP--FCG 436
Cdd:PRK07638 290 E--LSFVTALVdeeserRPNSVGR---------PFHNVQ--------VRICNEAGEEVQKGEIG----TVYVKSPqfFMG 346
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 437 YAGSKTQSEKKilrdvfrKGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTV 516
Cdd:PRK07638 347 YIIGGVLAREL-------NADGWMTVRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPAVDEIVVIGVPD 419
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 556957071 517 PeHEGRVGMAAIKLKEGKKfdgpNIYKHVMEYLPNYARPRFVRIQEAIEITGTFK 571
Cdd:PRK07638 420 S-YWGEKPVAIIKGSATKQ----QLKSFCLQRLSSFKIPKEWHFVDEIPYTNSGK 469
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
82-576 |
9.27e-25 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 108.25 E-value: 9.27e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 82 EVDKRSNQVARALQrEAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKVLIAGADLK 161
Cdd:PRK12406 16 ELAQRAARAAGGLA-ALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARVLIAHADLL 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 162 GAVEEVLPNlrenNVTIFYLNDEP-ITQGVeslkeKIEASSDQPIP---------KSFRITTTTKTAA--LYIYTSGTTG 229
Cdd:PRK12406 95 HGLASALPA----GVTVLSVPTPPeIAAAY-----RISPALLTPPAgaidwegwlAQQEPYDGPPVPQpqSMIYTSGTTG 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 230 LPK-----AAIINQRRLWGASFLLsISGVTSEDVVYTPLPLYHSAGLLLGVNGcIELGTTCVLRRKFSASQFWNDCREYN 304
Cdd:PRK12406 166 HPKgvrraAPTPEQAAAAEQMRAL-IYGLKPGIRALLTGPLYHSAPNAYGLRA-GRLGGVLVLQPRFDPEELLQLIERHR 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 305 TTAIQYIGEMLRYLCSTPRKdndrdhkVRIAVG-NGLR----------ADVWKEFLNRFGKIrICEFYAATE-GNIAFIN 372
Cdd:PRK12406 244 ITHMHMVPTMFIRLLKLPEE-------VRAKYDvSSLRhvihaaapcpADVKRAMIEWWGPV-IYEYYGSTEsGAVTFAT 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 373 ---YSGKIGAVGRAnflhkrgVPYELIKFntekeepvRDANGHciHVSKGETGLLVAKITTRSPFCgYAGsktQSEKKil 449
Cdd:PRK12406 316 sedALSHPGTVGKA-------APGAELRF--------VDEDGR--PLPQGEIGEIYSRIAGNPDFT-YHN---KPEKR-- 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 450 RDVFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGvtVPEHE-GRVGMAAI 528
Cdd:PRK12406 373 AEIDRGG--FITSGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFG--IPDAEfGEALMAVV 448
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 556957071 529 KLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAI--EITGTFKQRKVK 576
Cdd:PRK12406 449 EPQPGATLDEADIRAQLKARLAGYKVPKHIEIMAELprEDSGKIFKRRLR 498
|
|
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
67-564 |
1.26e-24 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 106.99 E-value: 1.26e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 67 HKPFLVFEKQVYTYLEVDKRSNQVARALQREAALKEGDTAALFLGNEPAYIwiwlgLAKLGCSMAclncnirsraflhsf 146
Cdd:cd05941 1 DRIAIVDDGDSITYADLVARAARLANRLLALGKDLRGDRVAFLAPPSAEYV-----VAQLAIWRA--------------- 60
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 147 ncSGAKVLIAgadLKGAVEEVLPNLRENNVTIFyLNDepitqgveslkekieassdqpipksfrittttktaALYIYTSG 226
Cdd:cd05941 61 --GGVAVPLN---PSYPLAELEYVITDSEPSLV-LDP-----------------------------------ALILYTSG 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 227 TTGLPKAAIINQRRLwgASFLLSIS---GVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWNDCREY 303
Cdd:cd05941 100 TTGRPKGVVLTHANL--AANVRALVdawRWTEDDVLLHVLPLHHVHGLVNALLCPLFAGASVEFLPKFDPKEVAISRLMP 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 304 NTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNGLR----------ADVWKEFLNRFGKiRICEFYAATEGNIAFIN- 372
Cdd:cd05941 178 SITVFMGVPTIYTRLLQYYEAHFTDPQFARAAAAERLRlmvsgsaalpVPTLEEWEAITGH-TLLERYGMTEIGMALSNp 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 373 YSG--KIGAVGRAnflhKRGVPYELIKFNTEKEEPVRDanghcihvskgetgllVAKITTRSP--FCGY--AGSKTQSEk 446
Cdd:cd05941 257 LDGerRPGTVGMP----LPGVQARIVDEETGEPLPRGE----------------VGEIQVRGPsvFKEYwnKPEATKEE- 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 447 kilrdvFRkGDIFFNSGDLLMIDHDNFIYFKDRIG-DTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHEGRVgM 525
Cdd:cd05941 316 ------FT-DDGWFKTGDLGVVDEDGYYWILGRSSvDIIKSGGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERV-V 387
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 556957071 526 AAIKLKEGKK-FDGPNIYKHVMEYLPNYARPRFVRIQEAI 564
Cdd:cd05941 388 AVVVLRAGAAaLSLEELKEWAKQRLAPYKRPRRLILVDEL 427
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
223-577 |
1.73e-24 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 105.05 E-value: 1.73e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 223 YTSGTTGLPKAAIINQRRLWGASFLLSIS-GVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVL-RRKFSASQFWNDC 300
Cdd:cd05917 9 FTSGTTGSPKGATLTHHNIVNNGYFIGERlGLTEQDRLCIPVPLFHCFGSVLGVLACLTHGATMVFpSPSFDPLAVLEAI 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 301 REYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVR--IAVGNGLRADVWKEFLNRFG--KIRICefYAATEGN-IAFINYSG 375
Cdd:cd05917 89 EKEKCTALHGVPTMFIAELEHPDFDKFDLSSLRtgIMAGAPCPPELMKRVIEVMNmkDVTIA--YGMTETSpVSTQTRTD 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 376 -----KIGAVGRAnflhkrgVPYELIKF--NTEKEEPVRDANGH-CIHvskgetGLLVAKittrspfcGYAGSKTQSEKK 447
Cdd:cd05917 167 dsiekRVNTVGRI-------MPHTEAKIvdPEGGIVPPVGVPGElCIR------GYSVMK--------GYWNDPEKTAEA 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 448 ILRDVFRKgdiffnSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVtvPEHE-GRVGMA 526
Cdd:cd05917 226 IDGDGWLH------TGDLAVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVGV--PDERyGEEVCA 297
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|.
gi 556957071 527 AIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKL 577
Cdd:cd05917 298 WIRLKEGAELTEEDIKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKL 348
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
78-577 |
4.61e-24 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 105.91 E-value: 4.61e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 78 YTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKVLIAG 157
Cdd:cd05959 30 LTYAELEAEARRVAGAL-RALGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPVPVNTLLTPDDYAYYLEDSRARVVVVS 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 158 ADLKGAVEEVLPNLRENNVTIFYLNDEPITQGVESLKEKIEASSDQPIPKSfritTTTKTAALYIYTSGTTGLPKAAIIN 237
Cdd:cd05959 109 GELAPVLAAALTKSEHTLVVLIVSGGAGPEAGALLLAELVAAEAEQLKPAA----THADDPAFWLYSSGSTGRPKGVVHL 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 238 QRRLWGAS--FLLSISGVTSEDVVYTPLPLYHSAGllLGvNGCI---ELGTTCVLRRKF-SASQFWNDCREYNTTAIQYI 311
Cdd:cd05959 185 HADIYWTAelYARNVLGIREDDVCFSAAKLFFAYG--LG-NSLTfplSVGATTVLMPERpTPAAVFKRIRRYRPTVFFGV 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 312 GEMLRYLCSTPRKDNDRDHKVRIAV--GNGLRADVWKEFLNRFGkIRICEFYAATE-GNIAFINYSGKI--GAVGRAnfl 386
Cdd:cd05959 262 PTLYAAMLAAPNLPSRDLSSLRLCVsaGEALPAEVGERWKARFG-LDILDGIGSTEmLHIFLSNRPGRVryGTTGKP--- 337
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 387 hkrgVP-YELikfntekeePVRDANGHciHVSKGETGLLVakITTRSPFCGYAGSKTQSekkilRDVFRKGdiFFNSGDL 465
Cdd:cd05959 338 ----VPgYEV---------ELRDEDGG--DVADGEPGELY--VRGPSSATMYWNNRDKT-----RDTFQGE--WTRTGDK 393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 466 LMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHEGRVgMAAIKLKEGkkFDGP-----N 540
Cdd:cd05959 394 YVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVEDEDGLTKP-KAFVVLRPG--YEDSealeeE 470
|
490 500 510
....*....|....*....|....*....|....*..
gi 556957071 541 IYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKL 577
Cdd:cd05959 471 LKEFVKDRLAPYKYPRWIVFVDELPKTATGKIQRFKL 507
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
66-571 |
7.99e-24 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 105.81 E-value: 7.99e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 66 PHKPFLVFEKQVYTYLEVDKRSNQVARALQREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHS 145
Cdd:PRK08314 24 PDKTAIVFYGRAISYRELLEEAERLAGYLQQECGVRKGDRVLLYMQNSPQFVIAYYAILRANAVVVPVNPMNREEELAHY 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 146 FNCSGAKVLIAGADLKGAVEEVLPNLRENNVTIFYLNDEPITQGVESLKEKIEASSDQPIPKSFRITTTTKTA------- 218
Cdd:PRK08314 104 VTDSGARVAIVGSELAPKVAPAVGNLRLRHVIVAQYSDYLPAEPEIAVPAWLRAEPPLQALAPGGVVAWKEALaaglapp 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 219 ---------ALYIYTSGTTGLPKAAIINQRRLWGASFLLSI-SGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLR 288
Cdd:PRK08314 184 phtagpddlAVLPYTSGTTGVPKGCMHTHRTVMANAVGSVLwSNSTPESVVLAVLPLFHVTGMVHSMNAPIYAGATVVLM 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 289 RKfsasqfWNdcRE--------YNTTAIQYIGEMLRYLCSTPRKDnDRDHKVRIAVGNG---LRADVWKEFLNRFGkIRI 357
Cdd:PRK08314 264 PR------WD--REaaarlierYRVTHWTNIPTMVVDFLASPGLA-ERDLSSLRYIGGGgaaMPEAVAERLKELTG-LDY 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 358 CEFYAATEgNIAFINYS----GKIGAVGRANFlhkrGVPYELIKFNTEKEEPVrdanghcihvskGETGllvaKITTRSP 433
Cdd:PRK08314 334 VEGYGLTE-TMAQTHSNppdrPKLQCLGIPTF----GVDARVIDPETLEELPP------------GEVG----EIVVHGP 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 434 --FCGYAGSKTQSEKK-ILRDvfrkGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEAS 510
Cdd:PRK08314 393 qvFKGYWNRPEATAEAfIEID----GKRFFRTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAEVENLLYKHPAIQEAC 468
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 556957071 511 VygVTVP-EHEGRVGMAAIKLKEGK--KFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFK 571
Cdd:PRK08314 469 V--IATPdPRRGETVKAVVVLRPEArgKTTEEEIIAWAREHMAAYKYPRIVEFVDSLPKSGSGK 530
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
50-564 |
4.96e-23 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 103.08 E-value: 4.96e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 50 PVFTLLDKF-LQQVEKQPHKPFLV--FEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKL 126
Cdd:cd05904 2 PTDLPLDSVsFLFASAHPSRPALIdaATGRALTYAELERRVRRLAAGL-AKRGGRKGDVVLLLSPNSIEFPVAFLAVLSL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 127 GCSMACLNCNIRSRAFLHSFNCSGAKVLIAgadlkgaVEEVLPNLRENNVTIFYLNDEPITQGVESLKEKIEASSDQPIP 206
Cdd:cd05904 81 GAVVTTANPLSTPAEIAKQVKDSGAKLAFT-------TAELAEKLASLALPVVLLDSAEFDSLSFSDLLFEADEAEPPVV 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 207 KsfriTTTTKTAALyIYTSGTTGLPKAAIINQRRLWG--ASFLLSI-SGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGT 283
Cdd:cd05904 154 V----IKQDDVAAL-LYSSGTTGRSKGVMLTHRNLIAmvAQFVAGEgSNSDSEDVFLCVLPMFHIYGLSSFALGLLRLGA 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 284 TCVLRRKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPrKDNDRDHKVRIAVGNG---LRADVWKEFLNRFGKIRICEF 360
Cdd:cd05904 229 TVVVMPRFDLEELLAAIERYKVTHLPVVPPIVLALVKSP-IVDKYDLSSLRQIMSGaapLGKELIEAFRAKFPNVDLGQG 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 361 YAATEG------NIAFINYSGKIGAVGRAnflhKRGVPYELIKFNTEKEEPvrdanghcihvsKGETGllvaKITTRSP- 433
Cdd:cd05904 308 YGMTEStgvvamCFAPEKDRAKYGSVGRL----VPNVEAKIVDPETGESLP------------PNQTG----ELWIRGPs 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 434 -FCGYAGSKTQSEKKILRDVFRKgdiffnSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVy 512
Cdd:cd05904 368 iMKGYLNNPEATAATIDKEGWLH------TGDLCYIDEDGYLFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAAV- 440
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 556957071 513 gVTVPEHE-GRVGMAAIKLKEGKKFDGpniyKHVMEYLPN----YARPRFVRIQEAI 564
Cdd:cd05904 441 -IPYPDEEaGEVPMAFVVRKPGSSLTE----DEIMDFVAKqvapYKKVRKVAFVDAI 492
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
53-576 |
1.07e-22 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 102.39 E-value: 1.07e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 53 TLLDKFLQQVEKQPHKPFLVFEKQVYTYLEVDKrsnQVARALQ--REAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSM 130
Cdd:PRK05605 33 TLVDLYDNAVARFGDRPALDFFGATTTYAELGK---QVRRAAAglRALGVRPGDRVAIVLPNCPQHIVAFYAVLRLGAVV 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 131 ACLNCNIRSRAFLHSFNCSGAKVLIAGADLKGAVEEVLPNLR-ENNVTIFYLNDEPITQG------VESLKEKIEASS-- 201
Cdd:PRK05605 110 VEHNPLYTAHELEHPFEDHGARVAIVWDKVAPTVERLRRTTPlETIVSVNMIAAMPLLQRlalrlpIPALRKARAALTgp 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 202 -----------DQPIPKSFRI----TTTTKTAALYIYTSGTTGLPKAAIINQRRL---------WgasfllsISGVTSED 257
Cdd:PRK05605 190 apgtvpwetlvDAAIGGDGSDvshpRPTPDDVALILYTSGTTGKPKGAQLTHRNLfanaaqgkaW-------VPGLGDGP 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 258 -VVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAV 336
Cdd:PRK05605 263 eRVLAALPMFHAYGLTLCLTLAVSIGGELVLLPAPDIDLILDAMKKHPPTWLPGVPPLYEKIAEAAEERGVDLSGVRNAF 342
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 337 GNG--LRADVWKEFLNRFGKiRICEFYAATE------GNIafINYSGKIGAVGranflhkrgVPYElikfNTE-----KE 403
Cdd:PRK05605 343 SGAmaLPVSTVELWEKLTGG-LLVEGYGLTEtspiivGNP--MSDDRRPGYVG---------VPFP----DTEvrivdPE 406
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 404 EPVRDanghcihVSKGETGLLVAkittRSP--FCGYAGSKTQSEKKILRDVFRKGDIffnsgdlLMIDHDNFIYFKDRIG 481
Cdd:PRK05605 407 DPDET-------MPDGEEGELLV----RGPqvFKGYWNRPEETAKSFLDGWFRTGDV-------VVMEEDGFIRIVDRIK 468
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 482 DTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHEGRVgMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPR-FVRI 560
Cdd:PRK05605 469 ELIITGGFNVYPAEVEEVLREHPGVEDAAVVGLPREDGSEEV-VAAVVLEPGAALDPEGLRAYCREHLTRYKVPRrFYHV 547
|
570
....*....|....*..
gi 556957071 561 QE-AIEITGTFKQRKVK 576
Cdd:PRK05605 548 DElPRDQLGKVRRREVR 564
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
66-556 |
1.74e-22 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 101.37 E-value: 1.74e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 66 PHKPFLVFEKQVYTYLEVDKRSNQVARALQREAAlKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHS 145
Cdd:PRK13382 57 PDRPGLIDELGTLTWRELDERSDALAAALQALPI-GEPRVVGIMCRNHRGFVEALLAANRIGADILLLNTSFAGPALAEV 135
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 146 FNCSGAKVLIAGADLKGAVEEVLPNlRENNVTIFYLNDEPITQGVESLkekIEASSDQPIPKsfrittTTKTAALYIYTS 225
Cdd:PRK13382 136 VTREGVDTVIYDEEFSATVDRALAD-CPQATRIVAWTDEDHDLTVEVL---IAAHAGQRPEP------TGRKGRVILLTS 205
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 226 GTTGLPKAAiiNQRRLWGASFLLSISGVT---SEDVVYTPLPLYHSAG---LLLGVNgcieLGTTCVLRRKFSASQFWND 299
Cdd:PRK13382 206 GTTGTPKGA--RRSGPGGIGTLKAILDRTpwrAEEPTVIVAPMFHAWGfsqLVLAAS----LACTIVTRRRFDPEATLDL 279
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 300 CREYNTTAIQYIGEMLRYLCSTP----RKDNDRDHKVRIAVGNGLRADVWKEFLNRFGKIrICEFYAATEgniafinysg 375
Cdd:PRK13382 280 IDRHRATGLAVVPVMFDRIMDLPaevrNRYSGRSLRFAAASGSRMRPDVVIAFMDQFGDV-IYNNYNATE---------- 348
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 376 kIGAVGRANFLHKRGVPYELIKFNTEKEEPVRDANGHciHVSKGETGLLVAKITTRspFCGYAGSKTQSekkilrdvFRK 455
Cdd:PRK13382 349 -AGMIATATPADLRAAPDTAGRPAEGTEIRILDQDFR--EVPTGEVGTIFVRNDTQ--FDGYTSGSTKD--------FHD 415
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 456 GdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHEGRVGmAAIKLKEGKK 535
Cdd:PRK13382 416 G--FMASGDVGYLDENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRLA-AFVVLKPGAS 492
|
490 500
....*....|....*....|.
gi 556957071 536 FDGPNIYKHVMEYLPNYARPR 556
Cdd:PRK13382 493 ATPETLKQHVRDNLANYKVPR 513
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
68-577 |
2.00e-22 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 100.23 E-value: 2.00e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 68 KPFLVFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFN 147
Cdd:cd05919 1 KTAFYAADRSVTYGQLHDGANRLGSAL-RNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIAR 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 148 CSGAKVLIAGADlkgaveevlpnlrennvtifylndepitqgveslkekieassdqpipksfrittttkTAALYIYTSGT 227
Cdd:cd05919 80 DCEARLVVTSAD---------------------------------------------------------DIAYLLYSSGT 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 228 TGLPKAAIINQR------RLWGASFLlsisGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKF-SASQFWNDC 300
Cdd:cd05919 103 TGPPKGVMHAHRdpllfaDAMAREAL----GLTPGDRVFSSAKMFFGYGLGNSLWFPLAVGASAVLNPGWpTAERVLATL 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 301 REYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAV--GNGLRADVWKEFLNRFGkIRICEFYAATEGNIAFInySGKIG 378
Cdd:cd05919 179 ARFRPTVLYGVPTFYANLLDSCAGSPDALRSLRLCVsaGEALPRGLGERWMEHFG-GPILDGIGATEVGHIFL--SNRPG 255
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 379 AVgRANFLHKRGVPYELikfntekeePVRDANGHCIhvSKGETGLLVAKITTRSPfcGYAGSKTQSEKKILRDVFRKGDI 458
Cdd:cd05919 256 AW-RLGSTGRPVPGYEI---------RLVDEEGHTI--PPGEEGDLLVRGPSAAV--GYWNNPEKSRATFNGGWYRTGDK 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 459 FFnsgdllmIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVygVTVPEHEGRVGMAA-IKLKEGKKFD 537
Cdd:cd05919 322 FC-------RDADGWYTHAGRADDMLKVGGQWVSPVEVESLIIQHPAVAEAAV--VAVPESTGLSRLTAfVVLKSPAAPQ 392
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 556957071 538 GP---NIYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKL 577
Cdd:cd05919 393 ESlarDIHRHLLERLSAHKVPRRIAFVDELPRTATGKLQRFKL 435
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
66-577 |
3.69e-22 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 100.07 E-value: 3.69e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 66 PHKPFLVFEKQVYTYLEVDKRSNQVARALQReAALKEGDTAALFLGNEPAYIWIWLGLAKLGcsmACLNC-NIR----SR 140
Cdd:cd12118 18 PDRTSIVYGDRRYTWRQTYDRCRRLASALAA-LGISRGDTVAVLAPNTPAMYELHFGVPMAG---AVLNAlNTRldaeEI 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 141 AFLhsFNCSGAKVLIagadlkgaveevlpnlrennVTIFYLNDEPITQGVESLKEKIEASSDQPIpksfrittttktaAL 220
Cdd:cd12118 94 AFI--LRHSEAKVLF--------------------VDREFEYEDLLAEGDPDFEWIPPADEWDPI-------------AL 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 221 YiYTSGTTGLPKAAIINQRrlwGAsFLLSISGVTSEDV----VY-TPLPLYHSAG--LLLGVNGcieLGTTCVLRRKFSA 293
Cdd:cd12118 139 N-YTSGTTGRPKGVVYHHR---GA-YLNALANILEWEMkqhpVYlWTLPMFHCNGwcFPWTVAA---VGGTNVCLRKVDA 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 294 SQFWNDCREYNTTaiQYIGE--MLRYLCSTPRKDNDR-DHKVRIAVGNGLRADVWKEFLNRFGkIRICEFYAATE--GNI 368
Cdd:cd12118 211 KAIYDLIEKHKVT--HFCGAptVLNMLANAPPSDARPlPHRVHVMTAGAPPPAAVLAKMEELG-FDVTHVYGLTEtyGPA 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 369 AFINYSGK---IGAVGRANFLHKRGVPY----ELIKFNTEKEEPV-RDanghcihvskGET-------GLLVAKittrsp 433
Cdd:cd12118 288 TVCAWKPEwdeLPTEERARLKARQGVRYvgleEVDVLDPETMKPVpRD----------GKTigeivfrGNIVMK------ 351
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 434 fcGYAGSKTQSEKkilrdVFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYG 513
Cdd:cd12118 352 --GYLKNPEATAE-----AFRGG--WFHSGDLAVIHPDGYIEIKDRSKDIIISGGENISSVEVEGVLYKHPAVLEAAVVA 422
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 556957071 514 vtVP-EHEGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEaIEITGTFKQRKVKL 577
Cdd:cd12118 423 --RPdEKWGEVPCAFVELKEGAKVTEEEIIAFCREHLAGFMVPKTVVFGE-LPKTSTGKIQKFVL 484
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
78-577 |
5.64e-22 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 98.95 E-value: 5.64e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 78 YTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKVLIAG 157
Cdd:cd05972 1 WSFRELKRESAKAANVL-AKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVTD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 158 ADlkgaveevlpnlrennvtifylndepitqgveslkekieassdqpipksfrittttkTAALYIYTSGTTGLPKAAIIN 237
Cdd:cd05972 80 AE---------------------------------------------------------DPALIYFTSGTTGLPKGVLHT 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 238 QRrlWGASFLL---SISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVL--RRKFSASQFWNDCREYNTTAIQYIG 312
Cdd:cd05972 103 HS--YPLGHIPtaaYWLGLRPDDIHWNIADPGWAKGAWSSFFGPWLLGATVFVyeGPRFDAERILELLERYGVTSFCGPP 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 313 EMLRYLCSTPRKDNDRDH-KVRIAVGNGLR---ADVWKEFLNrfgkIRICEFYAATEGNIAFINYSG---KIGAVGRANF 385
Cdd:cd05972 181 TAYRMLIKQDLSSYKFSHlRLVVSAGEPLNpevIEWWRAATG----LPIRDGYGQTETGLTVGNFPDmpvKPGSMGRPTP 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 386 LHKrgvpYELIkfntekeepvrDANGHciHVSKGETGLLVAKITTRSPFCGYAGSKTQSEKKILRDVFRkgdiffnSGDL 465
Cdd:cd05972 257 GYD----VAII-----------DDDGR--ELPPGEEGDIAIKLPPPGLFLGYVGDPEKTEASIRGDYYL-------TGDR 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 466 LMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHeGRVGMAAIKLKEGkkFDGPN----- 540
Cdd:cd05972 313 AYRDEDGYFWFVGRADDIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPVR-GEVVKAFVVLTSG--YEPSEelaee 389
|
490 500 510
....*....|....*....|....*....|....*..
gi 556957071 541 IYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKL 577
Cdd:cd05972 390 LQGHVKKVLAPYKYPREIEFVEELPKTISGKIRRVEL 426
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
51-564 |
9.53e-22 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 98.80 E-value: 9.53e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 51 VFTLLDKFLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQREAaLKEGDTAALFLGNEPAYIWIWLGLAKLGCSM 130
Cdd:PRK06145 1 MFNLSASIAFHARRTPDRAALVYRDQEISYAEFHQRILQAAGMLHARG-IGQGDVVALLMKNSAAFLELAFAASYLGAVF 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 131 ACLNCNIRSRAFLHSFNCSGAKVLIAGADLkgaveEVLPNLRENNVTIfylnDEPITQGVESLkekieASSDQPIPKSFR 210
Cdd:PRK06145 80 LPINYRLAADEVAYILGDAGAKLLLVDEEF-----DAIVALETPKIVI----DAAAQADSRRL-----AQGGLEIPPQAA 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 211 ITTTTktaaLY--IYTSGTTGLPKAAIINQRRLWGASFLLSIS-GVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVL 287
Cdd:PRK06145 146 VAPTD----LVrlMYTSGTTDRPKGVMHSYGNLHWKSIDHVIAlGLTASERLLVVGPLYHVGAFDLPGIAVLWVGGTLRI 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 288 RRKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNGLRADVWK--EFLNRFGKIRICEFYAATE 365
Cdd:PRK06145 222 HREFDPEAVLAAIERHRLTCAWMAPVMLSRVLTVPDRDRFDLDSLAWCIGGGEKTPESRirDFTRVFTRARYIDAYGLTE 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 366 --GNIAFINYS---GKIGAVGRAnflhkrgVPYELIKfntekeepVRDANGHCIHVS-KGETGLLVAKITTrspfcGYAG 439
Cdd:PRK06145 302 tcSGDTLMEAGreiEKIGSTGRA-------LAHVEIR--------IADGAGRWLPPNmKGEICMRGPKVTK-----GYWK 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 440 SKTQSEKKILRDVFRKGDIFFnsgdllmIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEH 519
Cdd:PRK06145 362 DPEKTAEAFYGDWFRSGDVGY-------LDEEGFLYLTDRKKDMIISGGENIASSEVERVIYELPEVAEAAVIGVHDDRW 434
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 556957071 520 EGRVgMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAI 564
Cdd:PRK06145 435 GERI-TAVVVLNPGATLTLEALDRHCRQRLASFKVPRQLKVRDEL 478
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
42-585 |
1.04e-21 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 99.40 E-value: 1.04e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 42 MKKFEEKEPVFTLLDKFLQQVEKQPHKPFLVFEK----QVYTYLEVDKRSNQVARALQrEAALKEGDTAALFLGNEPAYI 117
Cdd:COG1022 1 MSEFSDVPPADTLPDLLRRRAARFPDRVALREKEdgiwQSLTWAEFAERVRALAAGLL-ALGVKPGDRVAILSDNRPEWV 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 118 WIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKVLIAG-ADLKGAVEEV---LPNLRennvTIFYLNDEPITQ--GVE 191
Cdd:COG1022 80 IADLAILAAGAVTVPIYPTSSAEEVAYILNDSGAKVLFVEdQEQLDKLLEVrdeLPSLR----HIVVLDPRGLRDdpRLL 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 192 SLKEKIEASSDQPIPKSF--RITTTTKTA-ALYIYTSGTTGLPKAAIINQRR-LWGASFLLSISGVTSEDVVYTPLPLYH 267
Cdd:COG1022 156 SLDELLALGREVADPAELeaRRAAVKPDDlATIIYTSGTTGRPKGVMLTHRNlLSNARALLERLPLGPGDRTLSFLPLAH 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 268 SAGLLLGVnGCIELGTTCVlrrkFSAS--QFWNDCREYNTTaiqyigemlrYLCSTPR---------------------- 323
Cdd:COG1022 236 VFERTVSY-YALAAGATVA----FAESpdTLAEDLREVKPT----------FMLAVPRvwekvyagiqakaeeagglkrk 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 324 -----------KDNDRDHKVRIAVGNGLR---AD--VWKEFLNRFG-KIR------------ICEFYAA----------- 363
Cdd:COG1022 301 lfrwalavgrrYARARLAGKSPSLLLRLKhalADklVFSKLREALGgRLRfavsggaalgpeLARFFRAlgipvlegygl 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 364 TE-GNIAFINYSG--KIGAVGRAnflhkrgVPyelikfNTEkeepVRdanghcIhvskGETG-LLVakittRSP--FCGY 437
Cdd:COG1022 381 TEtSPVITVNRPGdnRIGTVGPP-------LP------GVE----VK------I----AEDGeILV-----RGPnvMKGY 428
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 438 AGSKTQSEKKILRDvfrkGdiFFNSGDLLMIDHDNFIYFKDRIGDTFrwK---GENVATCEVADLLENVDFIQEASVYGv 514
Cdd:COG1022 429 YKNPEATAEAFDAD----G--WLHTGDIGELDEDGFLRITGRKKDLI--VtsgGKNVAPQPIENALKASPLIEQAVVVG- 499
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 515 tvpehegrvgmaaiklkEGKKFDG----PNiykhvMEYLPNYAR---------------PRFVR-IQEAI-EITGTFKQ- 572
Cdd:COG1022 500 -----------------DGRPFLAalivPD-----FEALGEWAEenglpytsyaelaqdPEVRAlIQEEVdRANAGLSRa 557
|
650
....*....|....*.
gi 556957071 573 ---RKVKLVEEGFDPA 585
Cdd:COG1022 558 eqiKRFRLLPKEFTIE 573
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
219-571 |
1.23e-21 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 96.56 E-value: 1.23e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 219 ALYIYTSGTTGLPKAAIINQRRLWGASFLLSISG--VTSEDVVYTPLPLYHSAGLLLgVNGCIELGTTCVLRRKF-SASQ 295
Cdd:cd17635 4 LAVIFTSGTTGEPKAVLLANKTFFAVPDILQKEGlnWVVGDVTYLPLPATHIGGLWW-ILTCLIHGGLCVTGGENtTYKS 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 296 FWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVR-IAVGNGLRADVWKEFLNRFGKIRICEFYAATE-GNIAFINY 373
Cdd:cd17635 83 LFKILTTNAVTTTCLVPTLLSKLVSELKSANATVPSLRlIGYGGSRAIAADVRFIEATGLTNTAQVYGLSEtGTALCLPT 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 374 ---SGKIGAVGRAnflhKRGVPYELIkfntekeepvrdaNGHCIHVSKGETGLLVAKiTTRSPFCGYAGSKTQSEKKIlr 450
Cdd:cd17635 163 dddSIEINAVGRP----YPGVDVYLA-------------ATDGIAGPSASFGTIWIK-SPANMLGYWNNPERTAEVLI-- 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 451 dvfrkgDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHEGRVGMAAIKL 530
Cdd:cd17635 223 ------DGWVNTGDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGELVGLAVVAS 296
|
330 340 350 360
....*....|....*....|....*....|....*....|.
gi 556957071 531 KEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFK 571
Cdd:cd17635 297 AELDENAIRALKHTIRRELEPYARPSTIVIVTDIPRTQSGK 337
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
35-580 |
1.42e-21 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 98.67 E-value: 1.42e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 35 TIPFNYKMKKFEEKEPVF---TLLDKFLQQVEKQPHKPFLVFEKQV-YTYLEVDKRSNQVARALQrEAALKEGDTAALFL 110
Cdd:PRK06087 3 TLTFNEQRRAAYRQQGYWgdaSLADYWQQTARAMPDKIAVVDNHGAsYTYSALDHAASRLANWLL-AKGIEPGDRVAFQL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 111 GNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKVLIAGA--------DLKGAVEEVLPNLREnnvTIFYLN 182
Cdd:PRK06087 82 PGWCEFTIIYLACLKVGAVSVPLLPSWREAELVWVLNKCQAKMFFAPTlfkqtrpvDLILPLQNQLPQLQQ---IVGVDK 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 183 DEPITQGVeSLKEKIEASSdqpiPKSFRITTTTKTAALYIYTSGTTGLPKAAIINQRR-LWGASFLLSISGVTSEDVVYT 261
Cdd:PRK06087 159 LAPATSSL-SLSQIIADYE----PLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNiLASERAYCARLNLTWQDVFMM 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 262 PLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWNDCREYNTT----AIQYIGEMLRYLcstprkdndRDHKVRIAV- 336
Cdd:PRK06087 234 PAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQRCTcmlgATPFIYDLLNLL---------EKQPADLSAl 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 337 ------GNGLRADVWKEFLNRfgKIRICEFYAATEGNI-AFINYSGKIGAVGranflHKRGVPYELIKfntekeepVRDA 409
Cdd:PRK06087 305 rfflcgGTTIPKKVARECQQR--GIKLLSVYGSTESSPhAVVNLDDPLSRFM-----HTDGYAAAGVE--------IKVV 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 410 NGHCIHVSKGETGllvaKITTRSP--FCGYAGSKTQSEkKILRDvfrkgDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWK 487
Cdd:PRK06087 370 DEARKTLPPGCEG----EEASRGPnvFMGYLDEPELTA-RALDE-----EGWYYSGDLCRMDEAGYIKITGRKKDIIVRG 439
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 488 GENVATCEVADLLENVDFIQEASVYGvtVP-EHEGRVGMAAIKLKEGKKfdGPNIyKHVMEYL-----PNYARPRFVRIQ 561
Cdd:PRK06087 440 GENISSREVEDILLQHPKIHDACVVA--MPdERLGERSCAYVVLKAPHH--SLTL-EEVVAFFsrkrvAKYKYPEHIVVI 514
|
570
....*....|....*....
gi 556957071 562 EAIEITGTFKQRKVKLVEE 580
Cdd:PRK06087 515 DKLPRTASGKIQKFLLRKD 533
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
55-580 |
8.19e-21 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 96.26 E-value: 8.19e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 55 LDKFLQQVEKQ-PHKPFLVFEKQVYTYLEVDKRSNQVARALQREAaLKEGDTAALFLGNEPAYIWIWLGLAKLGCSMAcl 133
Cdd:PRK07470 9 LAHFLRQAARRfPDRIALVWGDRSWTWREIDARVDALAAALAARG-VRKGDRILVHSRNCNQMFESMFAAFRLGAVWV-- 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 134 NCNIRSR----AFLHSfnCSGAKVLIAGADLKG---AVEEVLPNLReNNVTIfylNDEPITQGVESLkekIEASSDQPIP 206
Cdd:PRK07470 86 PTNFRQTpdevAYLAE--ASGARAMICHADFPEhaaAVRAASPDLT-HVVAI---GGARAGLDYEAL---VARHLGARVA 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 207 KSfriTTTTKTAALYIYTSGTTGLPKAAIINQRRLwgaSFLLS------ISGVTSEDVVYTPLPLYHSAGLLLGVNgcIE 280
Cdd:PRK07470 157 NA---AVDHDDPCWFFFTSGTTGRPKAAVLTHGQM---AFVITnhladlMPGTTEQDASLVVAPLSHGAGIHQLCQ--VA 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 281 LGTTCVL--RRKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNdRDH-KVRIAVGNG---LRADvWKEFLNRFGK 354
Cdd:PRK07470 229 RGAATVLlpSERFDPAEVWALVERHRVTNLFTVPTILKMLVEHPAVDR-YDHsSLRYVIYAGapmYRAD-QKRALAKLGK 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 355 IrICEFYAATE--GNIAFINYSG---------KIGAVGRAnflhkrgvpyelikfNTEKEEPVRDANGHciHVSKGETGl 423
Cdd:PRK07470 307 V-LVQYFGLGEvtGNITVLPPALhdaedgpdaRIGTCGFE---------------RTGMEVQIQDDEGR--ELPPGETG- 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 424 lvaKITTRSP--FCGYAGSKTQSEKkilrdVFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLE 501
Cdd:PRK07470 368 ---EICVIGPavFAGYYNNPEANAK-----AFRDG--WFRTGDLGHLDARGFLYITGRASDMYISGGSNVYPREIEEKLL 437
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 556957071 502 NVDFIQEASVYGVTVPEHeGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKvKLVEE 580
Cdd:PRK07470 438 THPAVSEVAVLGVPDPVW-GEVGVAVCVARDGAPVDEAELLAWLDGKVARYKLPKRFFFWDALPKSGYGKITK-KMVRE 514
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
53-579 |
1.79e-20 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 95.22 E-value: 1.79e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 53 TLLDKFLQQVEKQPHKPFLVFEKQV--YTYLEVDKRSNQVARALQReAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSM 130
Cdd:PRK12583 19 TIGDAFDATVARFPDREALVVRHQAlrYTWRQLADAVDRLARGLLA-LGVQPGDRVGIWAPNCAEWLLTQFATARIGAIL 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 131 ACLNCNIRSRAFLHSFNCSGAKVLIAGADLKGA-----VEEVLPNLRENNVTIFYLNDEPITQGVESL------------ 193
Cdd:PRK12583 98 VNINPAYRASELEYALGQSGVRWVICADAFKTSdyhamLQELLPGLAEGQPGALACERLPELRGVVSLapapppgflawh 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 194 --KEKIEASSDQPIPKsfRITTTTKTAALYI-YTSGTTGLPKAAIINQRRLWGASFLLSIS-GVTSEDVVYTPLPLYHSA 269
Cdd:PRK12583 178 elQARGETVSREALAE--RQASLDRDDPINIqYTSGTTGFPKGATLSHHNILNNGYFVAESlGLTEHDRLCVPVPLYHCF 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 270 GLLLGVNGCIELGTTCVL-RRKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVR--IAVGNGLRADVWK 346
Cdd:PRK12583 256 GMVLANLGCMTVGACLVYpNEAFDPLATLQAVEEERCTALYGVPTMFIAELDHPQRGNFDLSSLRtgIMAGAPCPIEVMR 335
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 347 EFLNRFGKIRICEFYAATEGniafinySGKIGAVGRANFLHKRgvpYELI-KFNTEKEEPVRDANGHCihVSKGETGllv 425
Cdd:PRK12583 336 RVMDEMHMAEVQIAYGMTET-------SPVSLQTTAADDLERR---VETVgRTQPHLEVKVVDPDGAT--VPRGEIG--- 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 426 aKITTR--SPFCGYAGSKTQSEKKIlrdvfrKGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENV 503
Cdd:PRK12583 401 -ELCTRgySVMKGYWNNPEATAESI------DEDGWMHTGDLATMDEQGYVRIVGRSKDMIIRGGENIYPREIEEFLFTH 473
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 556957071 504 DFIQEASVYGvtVP-EHEGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKLVE 579
Cdd:PRK12583 474 PAVADVQVFG--VPdEKYGEEIVAWVRLHPGHAASEEELREFCKARIAHFKVPRYFRFVDEFPMTVTGKVQKFRMRE 548
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
66-577 |
2.61e-20 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 94.69 E-value: 2.61e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 66 PHKPFLVFEK--QVYTYLEVDKRSNQVARALQrEAALKEGDTAALFLGNEP-AYIWIWLGLaKLGCSMACLNCNIRSRAF 142
Cdd:PRK13390 11 PDRPAVIVAEtgEQVSYRQLDDDSAALARVLY-DAGLRTGDVVALLSDNSPeALVVLWAAL-RSGLYITAINHHLTAPEA 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 143 LHSFNCSGAKVLIAGADLKGAVEEVLPNLRennVTIFYLNDepiTQGVESLKEKIEAS----SDQPipksfrittttkTA 218
Cdd:PRK13390 89 DYIVGDSGARVLVASAALDGLAAKVGADLP---LRLSFGGE---IDGFGSFEAALAGAgprlTEQP------------CG 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 219 ALYIYTSGTTGLPKAAiinQRRLWGASF------LLSIS----GVTSEDVVYTPLPLYHSAGLLLgVNGCIELGTTCVLR 288
Cdd:PRK13390 151 AVMLYSSGTTGFPKGI---QPDLPGRDVdapgdpIVAIArafyDISESDIYYSSAPIYHAAPLRW-CSMVHALGGTVVLA 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 289 RKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTprkdnDRDHKVRIAVGNgLRA----------DVWKEFLNRFGKIrIC 358
Cdd:PRK13390 227 KRFDAQATLGHVERYRITVTQMVPTMFVRLLKL-----DADVRTRYDVSS-LRAvihaaapcpvDVKHAMIDWLGPI-VY 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 359 EFYAATEGN-IAFIN---YSGKIGAVGRANF--LHkrgvpyelikfntekeepVRDANGHciHVSKGETGLLVAKiTTRS 432
Cdd:PRK13390 300 EYYSSTEAHgMTFIDspdWLAHPGSVGRSVLgdLH------------------ICDDDGN--ELPAGRIGTVYFE-RDRL 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 433 PFCGYAGSKTQSEKKILRDVFrkgdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVY 512
Cdd:PRK13390 359 PFRYLNDPEKTAAAQHPAHPF-----WTTVGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVHDVAVI 433
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 513 GVTVPEHEGRVgMAAIKLKEGkkFDGPN-IYKHVMEY----LPNYARPRFVRIQEAIEITGTFKQRKVKL 577
Cdd:PRK13390 434 GVPDPEMGEQV-KAVIQLVEG--IRGSDeLARELIDYtrsrIAHYKAPRSVEFVDELPRTPTGKLVKGLL 500
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
55-580 |
8.93e-20 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 93.17 E-value: 8.93e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 55 LDKFLQQV-EKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQReAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACL 133
Cdd:PRK06710 26 LHKYVEQMaSRYPEKKALHFLGKDITFSVFHDKVKRFANYLQK-LGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQT 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 134 NCNIRSRAFLHSFNCSGAKVLIAGADLKGAVEEVLPNLRENNVTIFYLND---------EPITQGVES-LKEKIEASSDQ 203
Cdd:PRK06710 105 NPLYTERELEYQLHDSGAKVILCLDLVFPRVTNVQSATKIEHVIVTRIADflpfpknllYPFVQKKQSnLVVKVSESETI 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 204 PIPKSFRITTTTKTA---------ALYIYTSGTTGLPKAAIINQRRL-----WGASFLLSIsgVTSEDVVYTPLPLYHSA 269
Cdd:PRK06710 185 HLWNSVEKEVNTGVEvpcdpendlALLQYTGGTTGFPKGVMLTHKNLvsntlMGVQWLYNC--KEGEEVVLGVLPFFHVY 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 270 GLLLGVNGCIELGTTCVLRRKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNG--LRADVWKE 347
Cdd:PRK06710 263 GMTAVMNLSIMQGYKMVLIPKFDMKMVFEAIKKHKVTLFPGAPTIYIALLNSPLLKEYDISSIRACISGSapLPVEVQEK 342
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 348 FlNRFGKIRICEFYAATEGNiafinysgkigAVGRANFLHKRGVPYELikfntekEEPVRDANGHCIHVSKGET--GLLV 425
Cdd:PRK06710 343 F-ETVTGGKLVEGYGLTESS-----------PVTHSNFLWEKRVPGSI-------GVPWPDTEAMIMSLETGEAlpPGEI 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 426 AKITTRSP--FCGYaGSKTQSEKKILRDVFrkgdifFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENV 503
Cdd:PRK06710 404 GEIVVKGPqiMKGY-WNKPEETAAVLQDGW------LHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPREVEEVLYEH 476
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 556957071 504 DFIQEASVYGVTVPeHEGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKLVEE 580
Cdd:PRK06710 477 EKVQEVVTIGVPDP-YRGETVKAFVVLKEGTECSEEELNQFARKYLAAYKVPKVYEFRDELPKTTVGKILRRVLIEE 552
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
60-562 |
1.89e-19 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 91.56 E-value: 1.89e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 60 QQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQrEAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRS 139
Cdd:PRK03640 10 QRAFLTPDRTAIEFEEKKVTFMELHEAVVSVAGKLA-ALGVKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSR 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 140 RAFLHSFNCSGAKVLIAGADLKGAVEEVlpnlrennvtifylndepITQGVESLKEkiEASSDQPIPKSFRITTTTKTaa 219
Cdd:PRK03640 89 EELLWQLDDAEVKCLITDDDFEAKLIPG------------------ISVKFAELMN--GPKEEAEIQEEFDLDEVATI-- 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 220 lyIYTSGTTGLPKAAIIN-QRRLWGA-SFLLSIsGVTSEDVVYTPLPLYHSAGLLLGVNGCIeLGTTCVLRRKFSASQFW 297
Cdd:PRK03640 147 --MYTSGTTGKPKGVIQTyGNHWWSAvGSALNL-GLTEDDCWLAAVPIFHISGLSILMRSVI-YGMRVVLVEKFDAEKIN 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 298 NDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNG------LRADVWKeflnrfgKIRICEFYAATE--GNIA 369
Cdd:PRK03640 223 KLLQTGGVTIISVVSTMLQRLLERLGEGTYPSSFRCMLLGGGpapkplLEQCKEK-------GIPVYQSYGMTEtaSQIV 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 370 FIN--YS-GKIGAVGRANFlhkrgvPYELikfNTEKEEPVRDANGHCIHVSKGETgllvakITTrspfcGYAgsktqSEK 446
Cdd:PRK03640 296 TLSpeDAlTKLGSAGKPLF------PCEL---KIEKDGVVVPPFEEGEIVVKGPN------VTK-----GYL-----NRE 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 447 KILRDVFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTvPEHEGRVGMA 526
Cdd:PRK03640 351 DATRETFQDG--WFKTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVP-DDKWGQVPVA 427
|
490 500 510
....*....|....*....|....*....|....*..
gi 556957071 527 AIKLkeGKKFDGPNIYKHVMEYLPNYARP-RFVRIQE 562
Cdd:PRK03640 428 FVVK--SGEVTEEELRHFCEEKLAKYKVPkRFYFVEE 462
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
81-579 |
1.98e-19 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 91.67 E-value: 1.98e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 81 LEVDKRSNQVARALQREAALKEGDTAALFLGNEPAYIWIWLGLaklGCSMACLNCNIRSRAFLHSFNCSGAKVLIAGADL 160
Cdd:cd05929 1 LEARDLDRAQVFHQRRLLLLDVYSIALNRNARAAAAEGVWIAD---GVYIYLINSILTVFAAAAAWKCGACPAYKSSRAP 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 161 KGAVEEVLPNLRENNVTIFYLNDEPITqGVESLKEKIEASSDQPIPksfRITTTTKTaalyIYTSGTTGLPK-------A 233
Cdd:cd05929 78 RAEACAIIEIKAAALVCGLFTGGGALD-GLEDYEAAEGGSPETPIE---DEAAGWKM----LYSGGTTGRPKgikrglpG 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 234 AIINQRRLWGASFLlsiSGVTSEDVVYTPLPLYHSAGLLLgVNGCIELGTTCVLRRKFSASQFWNDCREYNTTAIQYIGE 313
Cdd:cd05929 150 GPPDNDTLMAAALG---FGPGADSVYLSPAPLYHAAPFRW-SMTALFMGGTLVLMEKFDPEEFLRLIERYRVTFAQFVPT 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 314 MLRYLCSTPRKDNDRDH--KVRIAVGNGLRADVW-KEFLNRFGKIRICEFYAATEGN-IAFIN------YSGKIGAVGRA 383
Cdd:cd05929 226 MFVRLLKLPEAVRNAYDlsSLKRVIHAAAPCPPWvKEQWIDWGGPIIWEYYGGTEGQgLTIINgeewltHPGSVGRAVLG 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 384 NfLHkrgvpyelikfntekeepVRDANGHciHVSKGETGLLVakITTRSPFcGYAGSKTQSEKKILRDVFRkgdiffNSG 463
Cdd:cd05929 306 K-VH------------------ILDEDGN--EVPPGEIGEVY--FANGPGF-EYTNDPEKTAAARNEGGWS------TLG 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 464 DLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHeGRVGMAAI---KLKEGKKFDGPN 540
Cdd:cd05929 356 DVGYLDEDGYLYLTDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEEL-GQRVHAVVqpaPGADAGTALAEE 434
|
490 500 510
....*....|....*....|....*....|....*....
gi 556957071 541 IYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKLVE 579
Cdd:cd05929 435 LIAFLRDRLSRYKCPRSIEFVAELPRDDTGKLYRRLLRD 473
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
54-571 |
2.16e-19 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 92.13 E-value: 2.16e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 54 LLDKFLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACL 133
Cdd:PRK05677 26 IQAVLKQSCQRFADKPAFSNLGKTLTYGELYKLSGAFAAWLQQHTDLKPGDRIAVQLPNVLQYPVAVFGAMRAGLIVVNT 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 134 NCNIRSRAFLHSFNCSGAKVLIAGADLKGAVEEVLPNLRENNVTIFYLND--EP-----ITQGVESLKEKIEASSdqpIP 206
Cdd:PRK05677 106 NPLYTAREMEHQFNDSGAKALVCLANMAHLAEKVLPKTGVKHVIVTEVADmlPPlkrllINAVVKHVKKMVPAYH---LP 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 207 KSFRITT--------TTKTA-------ALYIYTSGTTGLPKAAIINQRRLWgASFLLSISGVTSE-----DVVYTPLPLY 266
Cdd:PRK05677 183 QAVKFNDalakgagqPVTEAnpqaddvAVLQYTGGTTGVAKGAMLTHRNLV-ANMLQCRALMGSNlnegcEILIAPLPLY 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 267 HSAGLLLGVNGCIELGTTCVL---RRKFSAsqFWNDCREYNTTAIQYIGEMLRYLCSTP--RKDNDRDHKVRIAVGNGLR 341
Cdd:PRK05677 262 HIYAFTFHCMAMMLIGNHNILisnPRDLPA--MVKELGKWKFSGFVGLNTLFVALCNNEafRKLDFSALKLTLSGGMALQ 339
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 342 ---ADVWKEFLNrfgkIRICEFYAATEGN-IAFINYSG--KIGAVGRAnflhkrgVPYELIKfntekeepVRDANGHciH 415
Cdd:PRK05677 340 latAERWKEVTG----CAICEGYGMTETSpVVSVNPSQaiQVGTIGIP-------VPSTLCK--------VIDDDGN--E 398
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 416 VSKGETGLLVAKittrSP--FCGYaGSKTQSEKKILRDvfrkgDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVAT 493
Cdd:PRK05677 399 LPLGEVGELCVK----GPqvMKGY-WQRPEATDEILDS-----DGWLKTGDIALIQEDGYMRIVDRKKDMILVSGFNVYP 468
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 556957071 494 CEVADLLENVDFIQEASVYGVTvPEHEGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFK 571
Cdd:PRK05677 469 NELEDVLAALPGVLQCAAIGVP-DEKSGEAIKVFVVVKPGETLTKEQVMEHMRANLTGYKVPKAVEFRDELPTTNVGK 545
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
64-580 |
3.05e-19 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 91.64 E-value: 3.05e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 64 KQPHKPFLVFEKQVYTYLEVDKRSNQVArALQREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFL 143
Cdd:PRK06178 45 ERPQRPAIIFYGHVITYAELDELSDRFA-ALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPVSPLFREHELS 123
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 144 HSFNCSGAKVLIAGADLKGAVEEVLPN--LRENNVTIF--YLNDEPI----------TQGVESLKEKIEASSDQPIPKSF 209
Cdd:PRK06178 124 YELNDAGAEVLLALDQLAPVVEQVRAEtsLRHVIVTSLadVLPAEPTlplpdslrapRLAAAGAIDLLPALRACTAPVPL 203
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 210 RITTTTKTAALYiYTSGTTGLPKAAIINQRRLWG--ASFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVL 287
Cdd:PRK06178 204 PPPALDALAALN-YTGGTTGMPKGCEHTQRDMVYtaAAAYAVAVVGGEDSVFLSFLPEFWIAGENFGLLFPLFSGATLVL 282
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 288 RRKFSASQFWNDCREY--NTTAI---QYIGEM---------LRYL----CSTPRKDNDRDHKVRiavgnglradvWKEFL 349
Cdd:PRK06178 283 LARWDAVAFMAAVERYrvTRTVMlvdNAVELMdhprfaeydLSSLrqvrVVSFVKKLNPDYRQR-----------WRALT 351
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 350 nrfGKIrICEF-YAATEGNIAFINYSG----KIGAVGRANFLhkrGVP-----YELIKFNTEKEEPVrdanghcihvskG 419
Cdd:PRK06178 352 ---GSV-LAEAaWGMTETHTCDTFTAGfqddDFDLLSQPVFV---GLPvpgteFKICDFETGELLPL------------G 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 420 ETGllvaKITTRSP--FCGYAGsKTQSEKKILRDvfrkGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVA 497
Cdd:PRK06178 413 AEG----EIVVRTPslLKGYWN-KPEATAEALRD----G--WLHTGDIGKIDEQGFLHYLGRRKEMLKVNGMSVFPSEVE 481
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 498 DLLENVDFIQEASVYGVTVPeHEGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRfVRIQEAIEITGTFKQRKVKL 577
Cdd:PRK06178 482 ALLGQHPAVLGSAVVGRPDP-DKGQVPVAFVQLKPGADLTAAALQAWCRENMAVYKVPE-IRIVDALPMTATGKVRKQDL 559
|
...
gi 556957071 578 VEE 580
Cdd:PRK06178 560 QAL 562
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
86-571 |
4.78e-19 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 90.19 E-value: 4.78e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 86 RSNQVARALQrEAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMAC----LNCNIRSRAFLHSFNCSGAKVLIAGADLK 161
Cdd:cd05922 2 GVSAAASALL-EAGGVRGERVVLILPNRFTYIELSFAVAYAGGRLGLvfvpLNPTLKESVLRYLVADAGGRIVLADAGAA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 162 GAVEEVLPNLRENNVTIfylnDEPITQGVESLKEKIEASSDQPipksfrittttktaALYIYTSGTTGLPKAAIINQRRL 241
Cdd:cd05922 81 DRLRDALPASPDPGTVL----DADGIRAARASAPAHEVSHEDL--------------ALLLYTSGSTGSPKLVRLSHQNL 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 242 -WGASFLLSISGVTSEDVVYTPLPLYHSAGLLLgVNGCIELGTTCVLRRKFSASQ-FWNDCREYNTTA---IQYIGEMLR 316
Cdd:cd05922 143 lANARSIAEYLGITADDRALTVLPLSYDYGLSV-LNTHLLRGATLVLTNDGVLDDaFWEDLREHGATGlagVPSTYAMLT 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 317 YLcstPRKDNDRDHKVRIA-VGNGLRADVWKEFLNRFGKIRICEFYAATEGNiAFINY------SGKIGAVGRAnflhkr 389
Cdd:cd05922 222 RL---GFDPAKLPSLRYLTqAGGRLPQETIARLRELLPGAQVYVMYGQTEAT-RRMTYlpperiLEKPGSIGLA------ 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 390 gVPyelikfNTEKEepVRDANGHCIHVskGETGllvaKITTRSPF--CGYAgsktqSEKKILRDVFRKGDIFFnSGDLLM 467
Cdd:cd05922 292 -IP------GGEFE--ILDDDGTPTPP--GEPG----EIVHRGPNvmKGYW-----NDPPYRRKEGRGGGVLH-TGDLAR 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 468 IDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHEGRVGMAAIKLKEgkkfDGPNIYKHVME 547
Cdd:cd05922 351 RDEDGFLFIVGRRDRMIKLFGNRISPTEIEAAARSIGLIIEAAAVGLPDPLGEKLALFVTAPDKI----DPKDVLRSLAE 426
|
490 500
....*....|....*....|....
gi 556957071 548 YLPNYARPRFVRIQEAIEITGTFK 571
Cdd:cd05922 427 RLPPYKVPATVRVVDELPLTASGK 450
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
65-564 |
6.24e-19 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 90.61 E-value: 6.24e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 65 QPHKPFLVFEKQVYTYLEVDKRSNQVARALQREAaLKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSR--AF 142
Cdd:PRK07786 30 QPDAPALRFLGNTTTWRELDDRVAALAGALSRRG-VGFGDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLTPPeiAF 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 143 LhsFNCSGAKVLIAG---ADLKGAVEEVLPNLRennvTIFYLNDEPiTQGVESLKEKIEASSDQPIPksfrITTTTKTAA 219
Cdd:PRK07786 109 L--VSDCGAHVVVTEaalAPVATAVRDIVPLLS----TVVVAGGSS-DDSVLGYEDLLAEAGPAHAP----VDIPNDSPA 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 220 LYIYTSGTTGLPKAAIINQRRLWGASF-LLSISGV-TSEDVVYTPLPLYHSAGLLLGVNGcIELGTTCVLR--RKFSASQ 295
Cdd:PRK07786 178 LIMYTSGTTGRPKGAVLTHANLTGQAMtCLRTNGAdINSDVGFVGVPLFHIAGIGSMLPG-LLLGAPTVIYplGAFDPGQ 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 296 FWNDCREYNTTAIQYIGEMLRYLCSTPRKDNdRDHKVR-IAVGNGLRAD-VWKEFLNRFGKIRICEFYAATE-------- 365
Cdd:PRK07786 257 LLDVLEAEKVTGIFLVPAQWQAVCAEQQARP-RDLALRvLSWGAAPASDtLLRQMAATFPEAQILAAFGQTEmspvtcml 335
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 366 -GNIAFinysGKIGAVGRanflhkrgvPYELIKFNTEKEEpVRDanghcihVSKGETGllvaKITTRSP--FCGYAGSKT 442
Cdd:PRK07786 336 lGEDAI----RKLGSVGK---------VIPTVAARVVDEN-MND-------VPVGEVG----EIVYRAPtlMSGYWNNPE 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 443 QSEkkilrDVFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYG--------V 514
Cdd:PRK07786 391 ATA-----EAFAGG--WFHSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVAVIGradekwgeV 463
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 556957071 515 TVPEHEGRVGMAAIKLKEGKKFdgpniykhVMEYLPNYARPRFVRIQEAI 564
Cdd:PRK07786 464 PVAVAAVRNDDAALTLEDLAEF--------LTDRLARYKHPKALEIVDAL 505
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
78-556 |
1.43e-18 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 88.17 E-value: 1.43e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 78 YTYLEVDKRSNQVARALQREAAlKEGDTAALFLGNEPAYIWIWLGLAKLGCsmaclncnirsraflhsfncsgakvliag 157
Cdd:cd05912 2 YTFAELFEEVSRLAEHLAALGV-RKGDRVALLSKNSIEMILLIHALWLLGA----------------------------- 51
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 158 adlkgavEEVLPNLRennvtifyLNDEPITQGVESLKEKIEAssdqpipksfrittttktAALYIYTSGTTGLPKAAIIN 237
Cdd:cd05912 52 -------EAVLLNTR--------LTPNELAFQLKDSDVKLDD------------------IATIMYTSGTTGKPKGVQQT 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 238 QRRLW----GASFLLsisGVTSEDVVYTPLPLYHSAGLLLGVNGCIElGTTCVLRRKFSASQFWNDCREYNTTAIQYIGE 313
Cdd:cd05912 99 FGNHWwsaiGSALNL---GLTEDDNWLCALPLFHISGLSILMRSVIY-GMTVYLVDKFDAEQVLHLINSGKVTIISVVPT 174
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 314 MLRYLCStprKDNDRDH-KVR-IAVGNG-LRADVWKEFLNRfgKIRICEFYAATEGNIAFINYS-----GKIGAVGRANF 385
Cdd:cd05912 175 MLQRLLE---ILGEGYPnNLRcILLGGGpAPKPLLEQCKEK--GIPVYQSYGMTETCSQIVTLSpedalNKIGSAGKPLF 249
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 386 lhkrGVPYELIKFNTEKEEPvrdanghcihvskGETGLLVAKITTrspfcGYAGSKTQSEKkilrdVFRKGdiFFNSGDL 465
Cdd:cd05912 250 ----PVELKIEDDGQPPYEV-------------GEILLKGPNVTK-----GYLNRPDATEE-----SFENG--WFKTGDI 300
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 466 LMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHeGRVGMAAIKLKegKKFDGPNIYKHV 545
Cdd:cd05912 301 GYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPAIKEAGVVGIPDDKW-GQVPVAFVVSE--RPISEEELIAYC 377
|
490
....*....|.
gi 556957071 546 MEYLPNYARPR 556
Cdd:cd05912 378 SEKLAKYKVPK 388
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
66-577 |
8.47e-18 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 86.92 E-value: 8.47e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 66 PHKPFLVFEKQVYTYLEVDKRSNQVARALQReAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHS 145
Cdd:PRK08162 32 PDRPAVIHGDRRRTWAETYARCRRLASALAR-RGIGRGDTVAVLLPNIPAMVEAHFGVPMAGAVLNTLNTRLDAASIAFM 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 146 FNCSGAKVLIAGADLKGAVEEVLPNLRENNVTIFYLNDEPITQG--VESLK-EKIEASSDQPIPKSFritTTTKTAALYI 222
Cdd:PRK08162 111 LRHGEAKVLIVDTEFAEVAREALALLPGPKPLVIDVDDPEYPGGrfIGALDyEAFLASGDPDFAWTL---PADEWDAIAL 187
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 223 -YTSGTTGLPKAAIINQRrlwGAsFLLSISGVTSEDV----VY--TpLPLYHSAG--------LLLGVNGCIelgttcvl 287
Cdd:PRK08162 188 nYTSGTTGNPKGVVYHHR---GA-YLNALSNILAWGMpkhpVYlwT-LPMFHCNGwcfpwtvaARAGTNVCL-------- 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 288 rRKFSASQFWNDCREYNTTaiQYIGE--MLRYLCSTPRKDND-RDHKVRIAVGNGLRADVWKEFLNRFGkIRICEFYAAT 364
Cdd:PRK08162 255 -RKVDPKLIFDLIREHGVT--HYCGApiVLSALINAPAEWRAgIDHPVHAMVAGAAPPAAVIAKMEEIG-FDLTHVYGLT 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 365 E--GNIAFINYSGKIGAV---GRANFLHKRGVPYELIK----FNTEKEEPV-RDanghcihvskGETgllVAKITTRSPF 434
Cdd:PRK08162 331 EtyGPATVCAWQPEWDALpldERAQLKARQGVRYPLQEgvtvLDPDTMQPVpAD----------GET---IGEIMFRGNI 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 435 C--GYAGSKTQSEKkilrdVFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVy 512
Cdd:PRK08162 398 VmkGYLKNPKATEE-----AFAGG--WFHTGDLAVLHPDGYIKIKDRSKDIIISGGENISSIEVEDVLYRHPAVLVAAV- 469
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 556957071 513 gVTVP-EHEGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEaIEITGTFKQRKVKL 577
Cdd:PRK08162 470 -VAKPdPKWGEVPCAFVELKDGASATEEEIIAHCREHLAGFKVPKAVVFGE-LPKTSTGKIQKFVL 533
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
76-578 |
1.88e-17 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 85.18 E-value: 1.88e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 76 QVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPayiwiwlglaklgcsmaclNCNIRSRAFLHSfncsgakvli 155
Cdd:cd05971 5 EKVTFKELKTASNRFANVL-KEIGLEKGDRVGVFLSQGP-------------------ECAIAHIAILRS---------- 54
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 156 agadlkGAVEEVLPnlrennvTIFylndepitqGVESLKEKIEASSDQPIpksfrITTTTKTAALYIYTSGTTGLPKAAI 235
Cdd:cd05971 55 ------GAIAVPLF-------ALF---------GPEALEYRLSNSGASAL-----VTDGSDDPALIIYTSGTTGPPKGAL 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 236 INQRRLWGasfllSISGVT--------SEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRR--KFSASQFWNDCREYNT 305
Cdd:cd05971 108 HAHRVLLG-----HLPGVQfpfnlfprDGDLYWTPADWAWIGGLLDVLLPSLYFGVPVLAHRmtKFDPKAALDLMSRYGV 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 306 TAIQYIGEMLRYLCSTPRKDNDRDHKVR-IAVGN---GLRADVW-KEFLNrfgkIRICEFYAATEGNIAFIN----YSGK 376
Cdd:cd05971 183 TTAFLPPTALKMMRQQGEQLKHAQVKLRaIATGGeslGEELLGWaREQFG----VEVNEFYGQTECNLVIGNcsalFPIK 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 377 IGAVGRAnflhkrgVPYELIKfntekeepVRDANGHciHVSKGETGLLVAKITTRSPFCGYAGSKTQSEKKIlrdvfrKG 456
Cdd:cd05971 259 PGSMGKP-------IPGHRVA--------IVDDNGT--PLPPGEVGEIAVELPDPVAFLGYWNNPSATEKKM------AG 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 457 DiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEhEGRVGMAAIKLKEGKKF 536
Cdd:cd05971 316 D-WLLTGDLGRKDSDGYFWYVGRDDDVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIPDPI-RGEIVKAFVVLNPGETP 393
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 556957071 537 D---GPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKLV 578
Cdd:cd05971 394 SdalAREIQELVKTRLAAHEYPREIEFVNELPRTATGKIRRRELR 438
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
79-571 |
2.23e-17 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 85.46 E-value: 2.23e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 79 TYLEVDKRSNQVARALQREAalKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAK-VLIAG 157
Cdd:cd05909 9 TYRKLLTGAIALARKLAKMT--KEGENVGVMLPPSAGGALANFALALSGKVPVMLNYTAGLRELRACIKLAGIKtVLTSK 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 158 ADLKGAVEEVLPNLrENNVTIFYLNDepITQGVeSLKEKIEASSDQPIPKS------FRITTTTKTAALYIYTSGTTGLP 231
Cdd:cd05909 87 QFIEKLKLHHLFDV-EYDARIVYLED--LRAKI-SKADKCKAFLAGKFPPKwllrifGVAPVQPDDPAVILFTSGSEGLP 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 232 KAAIINQRRLW-GASFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLrrkfsasqfwndcrEYNTTAIQY 310
Cdd:cd05909 163 KGVVLSHKNLLaNVEQITAIFDPNPEDVVFGALPFFHSFGLTGCLWLPLLSGIKVVF--------------HPNPLDYKK 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 311 IGEMLR-----YLCSTP--------RKDNDRDHKVRIAV--GNGLRADVWKEFLNRFGkIRICEFYAATEGN-IAFIN-- 372
Cdd:cd05909 229 IPELIYdkkatILLGTPtflrgyarAAHPEDFSSLRLVVagAEKLKDTLRQEFQEKFG-IRILEGYGTTECSpVISVNtp 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 373 -YSGKIGAVGRanflhkrgvPYELIKFNTEKEEpvrdanGHCiHVSKGETGLLVakitTRSP--FCGYAGSKTQSEKKIl 449
Cdd:cd05909 308 qSPNKEGTVGR---------PLPGMEVKIVSVE------THE-EVPIGEGGLLL----VRGPnvMLGYLNEPELTSFAF- 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 450 rdvfrkGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVyGVTVPehEGRVGMAAIK 529
Cdd:cd05909 367 ------GDGWYDTGDIGKIDGEGFLTITGRLSRFAKIAGEMVSLEAIEDILSEILPEDNEVA-VVSVP--DGRKGEKIVL 437
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 556957071 530 LKEGKKFDGPNIYKHVMEY-LPNYARPRFVRIQEAIEITGTFK 571
Cdd:cd05909 438 LTTTTDTDPSSLNDILKNAgISNLAKPSYIHQVEEIPLLGTGK 480
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
79-533 |
2.52e-17 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 85.25 E-value: 2.52e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 79 TYLEVDKRSNQVARALQREAaLKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHsfncsgakvLIAGA 158
Cdd:cd05923 30 TYSELRARIEAVAARLHARG-LRPGQRVAVVLPNSVEAVIALLALHRLGAVPALINPRLKAAELAE---------LIERG 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 159 DLKGAV----EEVLPNLRENNVTIFYLNDEPITQGVESLKEKIEASSDQPipksfrittttKTAALYIYTSGTTGLPKAA 234
Cdd:cd05923 100 EMTAAViavdAQVMDAIFQSGVRVLALSDLVGLGEPESAGPLIEDPPREP-----------EQPAFVFYTSGTTGLPKGA 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 235 IINQR-RLWGASFLLSISGVT--SEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWNDCREYNTTAI--- 308
Cdd:cd05923 169 VIPQRaAESRVLFMSTQAGLRhgRHNVVLGLMPLYHVIGFFAVLVAALALDGTYVVVEEFDPADALKLIEQERVTSLfat 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 309 -QYIGEMLRYLCSTPRKDNDRDHkvrIAVGNGLRADVWKEFLNRFGKIRICEFYAATEGNIAFINYSGKIGAVGRANFlh 387
Cdd:cd05923 249 pTHLDALAAAAEFAGLKLSSLRH---VTFAGATMPDAVLERVNQHLPGEKVNIYGTTEAMNSLYMRDARTGTEMRPGF-- 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 388 krgvpYELIKFntekeepVRDANGHCIHVSKGETGLLVAKITTRSPFCGYAGSKTQSEKKILrdvfrkgDIFFNSGDLLM 467
Cdd:cd05923 324 -----FSEVRI-------VRIGGSPDEALANGEEGELIVAAAADAAFTGYLNQPEATAKKLQ-------DGWYRTGDVGY 384
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 556957071 468 IDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTvPEHEGRVGMAAIKLKEG 533
Cdd:cd05923 385 VDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIGVA-DERWGQSVTACVVPREG 449
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
53-571 |
2.67e-17 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 85.31 E-value: 2.67e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 53 TLLDKFLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMAC 132
Cdd:PRK08751 26 TVAEVFATSVAKFADRPAYHSFGKTITYREADQLVEQFAAYLLGELQLKKGDRVALMMPNCLQYPIATFGVLRAGLTVVN 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 133 LNCNIRSRAFLHSFNCSGAKVLIAGADLKGAVEEVLPNLRENNVTIFYLND----------------------EPITQGV 190
Cdd:PRK08751 106 VNPLYTPRELKHQLIDSGASVLVVIDNFGTTVQQVIADTPVKQVITTGLGDmlgfpkaalvnfvvkyvkklvpEYRINGA 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 191 ESLKEKIEASSDQPIPKsfrITTTTKTAALYIYTSGTTGLPKAAIINQRRLWG----ASFLLSISGVTSE--DVVYTPLP 264
Cdd:PRK08751 186 IRFREALALGRKHSMPT---LQIEPDDIAFLQYTGGTTGVAKGAMLTHRNLVAnmqqAHQWLAGTGKLEEgcEVVITALP 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 265 LYH-----SAGLL-LGVNGCIELgttcvLRRKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGN 338
Cdd:PRK08751 263 LYHifaltANGLVfMKIGGCNHL-----ISNPRDMPGFVKELKKTRFTAFTGVNTLFNGLLNTPGFDQIDFSSLKMTLGG 337
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 339 GLR-----ADVWKeflnRFGKIRICEFYAATEGN-IAFIN------YSGKIgavgranflhkrGVPYelikfnTEKEEPV 406
Cdd:PRK08751 338 GMAvqrsvAERWK----QVTGLTLVEAYGLTETSpAACINpltlkeYNGSI------------GLPI------PSTDACI 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 407 RDANGHCIHVskGETGLLVAKittrSP--FCGYAGSKTQSEKKIlrdvfrKGDIFFNSGDLLMIDHDNFIYFKDRIGDTF 484
Cdd:PRK08751 396 KDDAGTVLAI--GEIGELCIK----GPqvMKGYWKRPEETAKVM------DADGWLHTGDIARMDEQGFVYIVDRKKDMI 463
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 485 RWKGENVATCEVADLLENVDFIQEasVYGVTVPEHEGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAI 564
Cdd:PRK08751 464 LVSGFNVYPNEIEDVIAMMPGVLE--VAAVGVPDEKSGEIVKVVIVKKDPALTAEDVKAHARANLTGYKQPRIIEFRKEL 541
|
....*..
gi 556957071 565 EITGTFK 571
Cdd:PRK08751 542 PKTNVGK 548
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
53-562 |
3.22e-17 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 85.11 E-value: 3.22e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 53 TLLDKFLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQREAALKEGDTAALFLGNEPAYIWIWLGLAKLGcsMAC 132
Cdd:PRK08974 24 SLVDMFEQAVARYADQPAFINMGEVMTFRKLEERSRAFAAYLQNGLGLKKGDRVALMMPNLLQYPIALFGILRAG--MIV 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 133 LNCN--IRSRAFLHSFNCSGAKVLIAGADLKGAVEEVLPNLRENNVTIFYLNDE-PITQG------VESLKeKIEASSDQ 203
Cdd:PRK08974 102 VNVNplYTPRELEHQLNDSGAKAIVIVSNFAHTLEKVVFKTPVKHVILTRMGDQlSTAKGtlvnfvVKYIK-RLVPKYHL 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 204 PIPKSFRITTTTKTAALYI-------------YTSGTTGLPKAAIINQRRL--------WGASFLLSisgvTSEDVVYTP 262
Cdd:PRK08974 181 PDAISFRSALHKGRRMQYVkpelvpedlaflqYTGGTTGVAKGAMLTHRNMlanleqakAAYGPLLH----PGKELVVTA 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 263 LPLYHSagLLLGVNgC---IELGTTCVL---RRKFSAsqFWNDCREYNTTAIQYIGEMLRYLCstprkDNDRDHKV---- 332
Cdd:PRK08974 257 LPLYHI--FALTVN-CllfIELGGQNLLitnPRDIPG--FVKELKKYPFTAITGVNTLFNALL-----NNEEFQELdfss 326
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 333 -RIAVGNGLR-----ADVWKEFLnrfgKIRICEFYAATE-------GNIAFINYSGKIGAvgranflhkrGVPYELIKfn 399
Cdd:PRK08974 327 lKLSVGGGMAvqqavAERWVKLT----GQYLLEGYGLTEcsplvsvNPYDLDYYSGSIGL----------PVPSTEIK-- 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 400 tekeepVRDANGHciHVSKGETGLLVAKittrSP--FCGYaGSKTQSEKKILRDvfrkGdiFFNSGDLLMIDHDNFIYFK 477
Cdd:PRK08974 391 ------LVDDDGN--EVPPGEPGELWVK----GPqvMLGY-WQRPEATDEVIKD----G--WLATGDIAVMDEEGFLRIV 451
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 478 DRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVtvpEHEgrVGMAAIKL---KEGKKFDGPNIYKHVMEYLPNYAR 554
Cdd:PRK08974 452 DRKKDMILVSGFNVYPNEIEDVVMLHPKVLEVAAVGV---PSE--VSGEAVKIfvvKKDPSLTEEELITHCRRHLTGYKV 526
|
....*...
gi 556957071 555 PRFVRIQE 562
Cdd:PRK08974 527 PKLVEFRD 534
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
219-577 |
3.71e-17 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 83.30 E-value: 3.71e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 219 ALYIYTSGTTGLPKAAiinQRRLWG----ASFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVL------R 288
Cdd:cd05944 5 AAYFHTGGTTGTPKLA---QHTHSNevynAWMLALNSLFDPDDVLLCGLPLFHVNGSVVTLLTPLASGAHVVLagpagyR 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 289 RKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPrkdNDRD-HKVRIAVGNG--LRADVWKEFLNRFGkIRICEFYAATE 365
Cdd:cd05944 82 NPGLFDNFWKLVERYRITSLSTVPTVYAALLQVP---VNADiSSLRFAMSGAapLPVELRARFEDATG-LPVVEGYGLTE 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 366 GN----IAFINYSGKIGAVGRAnflhkrgVPYELIKFNtekeepVRDANGHCI-HVSKGETGLLVakITTRSPFCGYags 440
Cdd:cd05944 158 ATclvaVNPPDGPKRPGSVGLR-------LPYARVRIK------VLDGVGRLLrDCAPDEVGEIC--VAGPGVFGGY--- 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 441 kTQSEKKilRDVFrKGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPeHE 520
Cdd:cd05944 220 -LYTEGN--KNAF-VADGWLNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDA-HA 294
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*...
gi 556957071 521 GRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYAR-PRFVRIQEAIEITGTFKQRKVKL 577
Cdd:cd05944 295 GELPVAYVQLKPGAVVEEEELLAWARDHVPERAAvPKHIEVLEELPVTAVGKVFKPAL 352
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
78-555 |
2.75e-16 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 82.19 E-value: 2.75e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 78 YTYLEVDKRSNQVARALQReAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKVLIAG 157
Cdd:cd17642 45 YSYAEYLEMSVRLAEALKK-YGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIYNERELDHSLNISKPTIVFCS 123
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 158 adlKGAVEEVLPNLRENNV--TIFYLNDEPITQGVESLKEKIEassdQPIPKSFRITTTT-------KTAALYIYTSGTT 228
Cdd:cd17642 124 ---KKGLQKVLNVQKKLKIikTIIILDSKEDYKGYQCLYTFIT----QNLPPGFNEYDFKppsfdrdEQVALIMNSSGST 196
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 229 GLPKAAIINQR----RLWGASFLLSISGVTSEDVVYTPLPLYHSAGLL--LGVNGCielGTTCVLRRKFSASQFWNDCRE 302
Cdd:cd17642 197 GLPKGVQLTHKnivaRFSHARDPIFGNQIIPDTAILTVIPFHHGFGMFttLGYLIC---GFRVVLMYKFEEELFLRSLQD 273
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 303 YNTTAIQYIGEMLRYLCSTPRKDN-DRDHKVRIAVGNG-LRADVWKEFLNRFGKIRICEFYAATEGNIAFI---NYSGKI 377
Cdd:cd17642 274 YKVQSALLVPTLFAFFAKSTLVDKyDLSNLHEIASGGApLSKEVGEAVAKRFKLPGIRQGYGLTETTSAILitpEGDDKP 353
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 378 GAVGRANFLHKRGVpyelIKFNTEKEEPVRDANGHCIhvsKGEtglLVAKittrspfcGYAGSKTQSEKKILRDVfrkgd 457
Cdd:cd17642 354 GAVGKVVPFFYAKV----VDLDTGKTLGPNERGELCV---KGP---MIMK--------GYVNNPEATKALIDKDG----- 410
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 458 iFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEhEGRVGMAAIKLKEGKKFD 537
Cdd:cd17642 411 -WLHSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGVAGIPDED-AGELPAAVVVLEAGKTMT 488
|
490
....*....|....*...
gi 556957071 538 gpniYKHVMEYLPNYARP 555
Cdd:cd17642 489 ----EKEVMDYVASQVST 502
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
79-577 |
3.42e-16 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 81.93 E-value: 3.42e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 79 TYLEVDKRSNQVARALQREAaLKEGDTAALFLGNEPAYIWIWLGLAKLGCSMAcLNCNIRSRAFLHSFNCSGAKVLIA-- 156
Cdd:PRK07529 60 TYAELLADVTRTANLLHSLG-VGPGDVVAFLLPNLPETHFALWGGEAAGIANP-INPLLEPEQIAELLRAAGAKVLVTlg 137
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 157 ---GADLKGAVEEV---LPNLRennvTIFYLNDEPITQGVESLKEKI-------------EASSDQPIPKSF--RITTTT 215
Cdd:PRK07529 138 pfpGTDIWQKVAEVlaaLPELR----TVVEVDLARYLPGPKRLAVPLirrkaharildfdAELARQPGDRLFsgRPIGPD 213
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 216 KTAAlYIYTSGTTGLPKAAiinQRRLWG----ASFLLSISGVTSEDVVYTPLPLYHsaglllgVNGCIELGTTCVLRRK- 290
Cdd:PRK07529 214 DVAA-YFHTGGTTGMPKLA---QHTHGNevanAWLGALLLGLGPGDTVFCGLPLFH-------VNALLVTGLAPLARGAh 282
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 291 --FSASQ----------FWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDhKVRIAVGNG--LRADVWKEFLNRFGkIR 356
Cdd:PRK07529 283 vvLATPQgyrgpgvianFWKIVERYRINFLSGVPTVYAALLQVPVDGHDIS-SLRYALCGAapLPVEVFRRFEAATG-VR 360
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 357 ICEFYAATEGN----IAFINYSGKIGAVG-RanflhkrgVPYELIKFntekeePVRDANGHCIH-VSKGETGLLVakITT 430
Cdd:PRK07529 361 IVEGYGLTEATcvssVNPPDGERRIGSVGlR--------LPYQRVRV------VILDDAGRYLRdCAVDEVGVLC--IAG 424
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 431 RSPFCGYagskTQSEKKilRDVFrKGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEAS 510
Cdd:PRK07529 425 PNVFSGY----LEAAHN--KGLW-LEDGWLNTGDLGRIDADGYFWLTGRAKDLIIRGGHNIDPAAIEEALLRHPAVALAA 497
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 556957071 511 VYGvtVPE-HEGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYAR-PRFVRIQEAIEITGTFKQRKVKL 577
Cdd:PRK07529 498 AVG--RPDaHAGELPVAYVQLKPGASATEAELLAFARDHIAERAAvPKHVRILDALPKTAVGKIFKPAL 564
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
222-558 |
1.51e-15 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 78.08 E-value: 1.51e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 222 IYTSGTTGLPKAAIINQRRLWGASF-LLSISGVTSEDVVYTPLPLYHSAGLLLGVnGCIELGTTCVLRRKFSASQFWNDC 300
Cdd:cd17637 6 IHTAAVAGRPRGAVLSHGNLIAANLqLIHAMGLTEADVYLNMLPLFHIAGLNLAL-ATFHAGGANVVMEKFDPAEALELI 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 301 REYNTTaiqYIGE---MLRYLCSTpRKDNDRDHKVRIAVGnGLRAdvwKEFLNRFGKIRICEFYAA-----TEGNIAFIN 372
Cdd:cd17637 85 EEEKVT---LMGSfppILSNLLDA-AEKSGVDLSSLRHVL-GLDA---PETIQRFEETTGATFWSLygqteTSGLVTLSP 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 373 YSGKIGAVGRAnflhkrgVPYELIKFNTEKEEPVRDanghcihvskGETGllvaKITTRSP--FCGYAGSKTQSEKkilr 450
Cdd:cd17637 157 YRERPGSAGRP-------GPLVRVRIVDDNDRPVPA----------GETG----EIVVRGPlvFQGYWNLPELTAY---- 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 451 dVFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWK--GENVATCEV-ADLLENVDfIQEASVYGVTVPEHeGRVGMAA 527
Cdd:cd17637 212 -TFRNG--WHHTGDLGRFDEDGYLWYAGRKPEKELIKpgGENVYPAEVeKVILEHPA-IAEVCVIGVPDPKW-GEGIKAV 286
|
330 340 350
....*....|....*....|....*....|.
gi 556957071 528 IKLKEGKKFDGPNIYKHVMEYLPNYARPRFV 558
Cdd:cd17637 287 CVLKPGATLTADELIEFVGSRIARYKKPRYV 317
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
52-316 |
2.39e-15 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 79.16 E-value: 2.39e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 52 FTLLDKFLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMA 131
Cdd:PRK07798 3 WNIADLFEAVADAVPDRVALVCGDRRLTYAELEERANRLAHYL-IAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPV 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 132 CLNCNIRSRAFLHSFNCSGAKVLIAGADLKGAVEEVLPNLrENNVTIFYLND---EPITQGVESLKEKIEASSDQP--IP 206
Cdd:PRK07798 82 NVNYRYVEDELRYLLDDSDAVALVYEREFAPRVAEVLPRL-PKLRTLVVVEDgsgNDLLPGAVDYEDALAAGSPERdfGE 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 207 KSfrittttkTAALY-IYTSGTTGLPKAAIINQRRLW-----GASFLLSISGVTSEDVVYT----------PL-PLYHSA 269
Cdd:PRK07798 161 RS--------PDDLYlLYTGGTTGMPKGVMWRQEDIFrvllgGRDFATGEPIEDEEELAKRaaagpgmrrfPApPLMHGA 232
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|
gi 556957071 270 GlLLGVNGCIELGTTCVL--RRKFSASQFWNDCREYNTTAIQYIGE-MLR 316
Cdd:PRK07798 233 G-QWAAFAALFSGQTVVLlpDVRFDADEVWRTIEREKVNVITIVGDaMAR 281
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
58-562 |
2.61e-15 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 78.91 E-value: 2.61e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 58 FLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNI 137
Cdd:cd17655 3 FEEQAEKTPDHTAVVFEDQTLTYRELNERANQLARTL-REKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPIDPDY 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 138 RSRAFLHSFNCSGAKVLIAgadlkgavEEVLPNLRENNVTIFYLNDEPItqgveslkeKIEASSDQPIPKSFRittttkT 217
Cdd:cd17655 82 PEERIQYILEDSGADILLT--------QSHLQPPIAFIGLIDLLDEDTI---------YHEESENLEPVSKSD------D 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 218 AALYIYTSGTTGLPKAAIINQRRL----WGASFLLSISGVTSedvvytpLPLYHS-----------AGLLLGvngcielG 282
Cdd:cd17655 139 LAYVIYTSGSTGKPKGVMIEHRGVvnlvEWANKVIYQGEHLR-------VALFASisfdasvteifASLLSG-------N 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 283 TTCVLRRK--FSASQFWNDCREYNTTAIQYIGEMLRYLcstPRKDNDRDHKVR--IAVGNGLRADVWKEFLNRFG-KIRI 357
Cdd:cd17655 205 TLYIVRKEtvLDGQALTQYIRQNRITIIDLTPAHLKLL---DAADDSEGLSLKhlIVGGEALSTELAKKIIELFGtNPTI 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 358 CEFYAATEGNI--AFINYSGK--------IG---AVGRANFLHKRGVPyelikfntekeEPVRDANGHCIhvskgeTGLL 424
Cdd:cd17655 282 TNAYGPTETTVdaSIYQYEPEtdqqvsvpIGkplGNTRIYILDQYGRP-----------QPVGVAGELYI------GGEG 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 425 VAKittrspfcGYAGSKTQSEKKILRDVFRKGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVD 504
Cdd:cd17655 345 VAR--------GYLNRPELTAEKFVDDPFVPGERMYRTGDLARWLPDGNIEFLGRIDHQVKIRGYRIELGEIEARLLQHP 416
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 556957071 505 FIQEASVygVTVPEHEGRVGMAAIKLKEgKKFDGPNIYKHVMEYLPNYARPR-FVRIQE 562
Cdd:cd17655 417 DIKEAVV--IARKDEQGQNYLCAYIVSE-KELPVAQLREFLARELPDYMIPSyFIKLDE 472
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
78-577 |
9.18e-15 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 76.75 E-value: 9.18e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 78 YTYLEVDKRSNQVARALQREAALKEGDTAALFLGNEPAYIWIWLGLAKLGcsmaclncnirsraflhsfncsgakvliag 157
Cdd:cd05958 11 WTYRDLLALANRIANVLVGELGIVPGNRVLLRGSNSPELVACWFGIQKAG------------------------------ 60
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 158 adlkGAVEEVLPNLRENNVTiFYLNDEPITQGVesLKEKIEASSDqpipksfrittttktAALYIYTSGTTGLPKAAIIN 237
Cdd:cd05958 61 ----AIAVATMPLLRPKELA-YILDKARITVAL--CAHALTASDD---------------ICILAFTSGTTGAPKATMHF 118
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 238 QR------RLWGASFLlsisGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWNDCREYNTTAIQYI 311
Cdd:cd05958 119 HRdplasaDRYAVNVL----RLREDDRFVGSPPLAFTFGLGGVLLFPFGVGASGVLLEEATPDLLLSAIARYKPTVLFTA 194
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 312 GEMLRYLCSTPRKDNDRDHKVRIAV--GNGLRADVWKEFLNRFGkIRICEFYAATEGNIAFINYSG---KIGAVGRAnfl 386
Cdd:cd05958 195 PTAYRAMLAHPDAAGPDLSSLRKCVsaGEALPAALHRAWKEATG-IPIIDGIGSTEMFHIFISARPgdaRPGATGKP--- 270
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 387 hkrgVP-YELikfntekeePVRDANGHciHVSKGETGLLVAKITTrspFCGYAGSKTQsekkilRDVFRKGdiFFNSGDL 465
Cdd:cd05958 271 ----VPgYEA---------KVVDDEGN--PVPDGTIGRLAVRGPT---GCRYLADKRQ------RTYVQGG--WNITGDT 324
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 466 LMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEhEGRVGMAAIKLKEGKKfDGP----NI 541
Cdd:cd05958 325 YSRDPDGYFRHQGRSDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDES-RGVVVKAFVVLRPGVI-PGPvlarEL 402
|
490 500 510
....*....|....*....|....*....|....*.
gi 556957071 542 YKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKL 577
Cdd:cd05958 403 QDHAKAHIAPYKYPRAIEFVTELPRTATGKLQRFAL 438
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
78-577 |
9.24e-15 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 77.29 E-value: 9.24e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 78 YTYLEVDKRSNQVARALQReAALKEGDTAALFLGNEPAYIWIWLGLAKLGCsmACLNCNIRsrafLHS------FNCSGA 151
Cdd:cd12119 26 YTYAEVAERARRLANALRR-LGVKPGDRVATLAWNTHRHLELYYAVPGMGA--VLHTINPR----LFPeqiayiINHAED 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 152 KVLIAGADLKGAVEEVLPNLRENNVTIFYLND----EPITQGVESLKEKIEASSDQPIPKSFRitttTKTAALYIYTSGT 227
Cdd:cd12119 99 RVVFVDRDFLPLLEAIAPRLPTVEHVVVMTDDaampEPAGVGVLAYEELLAAESPEYDWPDFD----ENTAAAICYTSGT 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 228 TGLPKAAIINQRRLW--GASFLLSIS-GVTSEDVVYTPLPLYHsaglllgVNG------CIELGTTCVLRRKF----SAS 294
Cdd:cd12119 175 TGNPKGVVYSHRSLVlhAMAALLTDGlGLSESDVVLPVVPMFH-------VNAwglpyaAAMVGAKLVLPGPYldpaSLA 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 295 QFWndcREYNTTAIQYIGEMLRYLCSTP-RKDNDRDHKVRIAVGNG-LRADVWKEFLNRFgkIRICEFYAATE----GNI 368
Cdd:cd12119 248 ELI---EREGVTFAAGVPTVWQGLLDHLeANGRDLSSLRRVVIGGSaVPRSLIEAFEERG--VRVIHAWGMTEtsplGTV 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 369 AFIN-YSGKIGAVGRANFLHKRGVPYELIKFNTEKEEPVR-DANGhcihVSKGEtgLLVakittRSPFC--GYAGSKTQS 444
Cdd:cd12119 323 ARPPsEHSNLSEDEQLALRAKQGRPVPGVELRIVDDDGRElPWDG----KAVGE--LQV-----RGPWVtkSYYKNDEES 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 445 EKKilrdvFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEvadlLENV----DFIQEASVYGVtvpEHE 520
Cdd:cd12119 392 EAL-----TEDG--WLRTGDVATIDEDGYLTITDRSKDVIKSGGEWISSVE----LENAimahPAVAEAAVIGV---PHP 457
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 556957071 521 --GRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKL 577
Cdd:cd12119 458 kwGERPLAVVVLKEGATVTAEELLEFLADKVAKWWLPDDVVFVDEIPKTSTGKIDKKAL 516
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
53-532 |
1.38e-14 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 76.59 E-value: 1.38e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 53 TLLDKFLQQVEKQPHKPFLVFE--KQVYTYLEVDKRSNQVARALQREAAlKEGDTAALFLGNEPAYIWIWLGLAKLGCSM 130
Cdd:PRK05857 15 TVLDRVFEQARQQPEAIALRRCdgTSALRYRELVAEVGGLAADLRAQSV-SRGSRVLVISDNGPETYLSVLACAKLGAIA 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 131 ACLNCNIRSrAFLHSFN--CSGAKVLIAGADLKGAveEVLPNLrennvtifyLNDEP-ITQGVESLKEKIEASSDQPIPK 207
Cdd:PRK05857 94 VMADGNLPI-AAIERFCqiTDPAAALVAPGSKMAS--SAVPEA---------LHSIPvIAVDIAAVTRESEHSLDAASLA 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 208 SfRITTTTKTAALYIYTSGTTGLPKAAIINQRRLWGASFLLSISGVTSEDVV-----YTPLPLYHSAGLLLGVNgCIELG 282
Cdd:PRK05857 162 G-NADQGSEDPLAMIFTSGTTGEPKAVLLANRTFFAVPDILQKEGLNWVTWVvgettYSPLPATHIGGLWWILT-CLMHG 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 283 TTCVLRRKFSAS--QFWNDcREYNTTAIqyIGEMLRYLCSTPRKDNDRDHKVRIAVGNGLR---ADVwkEFLNRFGkIRI 357
Cdd:PRK05857 240 GLCVTGGENTTSllEILTT-NAVATTCL--VPTLLSKLVSELKSANATVPSLRLVGYGGSRaiaADV--RFIEATG-VRT 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 358 CEFYAATE------------GNIAFInysgKIGAVGRanflhkrgvPYELIkfntekEEPVRDANGHCIHVSKGETGLLV 425
Cdd:PRK05857 314 AQVYGLSEtgctalclptddGSIVKI----EAGAVGR---------PYPGV------DVYLAATDGIGPTAPGAGPSASF 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 426 AKITTRSP--FCGYAGSKTQSekkilRDVFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENV 503
Cdd:PRK05857 375 GTLWIKSPanMLGYWNNPERT-----AEVLIDG--WVNTGDLLERREDGFFYIKGRSSEMIICGGVNIAPDEVDRIAEGV 447
|
490 500
....*....|....*....|....*....
gi 556957071 504 DFIQEASVYGVTVPEHEGRVGMAAIKLKE 532
Cdd:PRK05857 448 SGVREAACYEIPDEEFGALVGLAVVASAE 476
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
78-577 |
1.83e-14 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 76.00 E-value: 1.83e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 78 YTYLEVDKRSNQVARALQREAaLKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIrsraflhsfncsgakvliAG 157
Cdd:PRK09088 23 WTYAELDALVGRLAAVLRRRG-CVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRL------------------SA 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 158 ADLKGAVEEVLPNLrennvtifYLNDEPITQG---VESLkEKIEASSDQPIPkSFRITTTTKTAALYIYTSGTTGLPKAA 234
Cdd:PRK09088 84 SELDALLQDAEPRL--------LLGDDAVAAGrtdVEDL-AAFIASADALEP-ADTPSIPPERVSLILFTSGTSGQPKGV 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 235 IINQRRLWGASFLLSISG-VTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWNDCREYNTTAIQYIG- 312
Cdd:PRK09088 154 MLSERNLQQTAHNFGVLGrVDAHSSFLCDAPMFHIIGLITSVRPVLAVGGSILVSNGFEPKRTLGRLGDPALGITHYFCv 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 313 -EMLRYLCSTPRKDNDRDHKVRIAVGNG---LRADV--WKEflnrfGKIRICEFYAATEGNIAF------INYSGKIGAV 380
Cdd:PRK09088 234 pQMAQAFRAQPGFDAAALRHLTALFTGGaphAAEDIlgWLD-----DGIPMVDGFGMSEAGTVFgmsvdcDVIRAKAGAA 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 381 GranfLHKRGVPYELIkfntekeepvrDANGHciHVSKGETGLLVAKITTRSPfcGYAGSKTQSEKKilrdvfRKGDIFF 460
Cdd:PRK09088 309 G----IPTPTVQTRVV-----------DDQGN--DCPAGVPGELLLRGPNLSP--GYWRRPQATARA------FTGDGWF 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 461 NSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHeGRVGMAAIKLKEGKKFDGPN 540
Cdd:PRK09088 364 RTGDIARRDADGFFWVVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQW-GEVGYLAIVPADGAPLDLER 442
|
490 500 510
....*....|....*....|....*....|....*..
gi 556957071 541 IYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKL 577
Cdd:PRK09088 443 IRSHLSTRLAKYKVPKHLRLVDALPRTASGKLQKARL 479
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
53-286 |
3.09e-14 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 75.62 E-value: 3.09e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 53 TLLDKFLQQVEKQPHKPFLVFEKQ--VYTYLEVDKRSNQVARALQReAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSM 130
Cdd:PRK08315 17 TIGQLLDRTAARYPDREALVYRDQglRWTYREFNEEVDALAKGLLA-LGIEKGDRVGIWAPNVPEWVLTQFATAKIGAIL 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 131 ACLNCNIRSRAFLHSFNCSGAKVLIAGADLKGA-----VEEVLPNLRENNV------------TIFYLNDEPiTQGVESL 193
Cdd:PRK08315 96 VTINPAYRLSELEYALNQSGCKALIAADGFKDSdyvamLYELAPELATCEPgqlqsarlpelrRVIFLGDEK-HPGMLNF 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 194 KEKIEASSDQPIPKSFRITTTTK-TAALYI-YTSGTTGLPKAA------IINQRRLWGASFLLsisgvTSEDVVYTPLPL 265
Cdd:PRK08315 175 DELLALGRAVDDAELAARQATLDpDDPINIqYTSGTTGFPKGAtlthrnILNNGYFIGEAMKL-----TEEDRLCIPVPL 249
|
250 260
....*....|....*....|.
gi 556957071 266 YHSAGLLLGVNGCIELGTTCV 286
Cdd:PRK08315 250 YHCFGMVLGNLACVTHGATMV 270
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
78-573 |
5.05e-14 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 74.48 E-value: 5.05e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 78 YTYLEVDKRSNQVARALQrEAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKVLIAG 157
Cdd:cd05973 1 LTFGELRALSARFANALQ-ELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLVVTD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 158 ADlkgaveevlpNLREnnvtifyLNDEPItqgveslkekieassdqpipksfrittttktaaLYIYTSGTTGLPKAAIIN 237
Cdd:cd05973 80 AA----------NRHK-------LDSDPF---------------------------------VMMFTSGTTGLPKGVPVP 109
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 238 QRRL--WGAsFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGT-TCVLRRKFSASQFWNDCREYNTTAIQYIGEM 314
Cdd:cd05973 110 LRALaaFGA-YLRDAVDLRPEDSFWNAADPGWAYGLYYAITGPLALGHpTILLEGGFSVESTWRVIERLGVTNLAGSPTA 188
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 315 LRYLCSTPRKDNDRDhKVRIAV----GNGLRADVWKEFLNRFGkIRICEFYAATEGNIAFINYSG-----KIGAVGRAnf 385
Cdd:cd05973 189 YRLLMAAGAEVPARP-KGRLRRvssaGEPLTPEVIRWFDAALG-VPIHDHYGQTELGMVLANHHAlehpvHAGSAGRA-- 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 386 lhkrgVP-YELIKFNTEKEEPVrdanghcihvsKGETGLLVAKItTRSPFCGYAGSKTQSEKKILRDVFRkgdiffnSGD 464
Cdd:cd05973 265 -----MPgWRVAVLDDDGDELG-----------PGEPGRLAIDI-ANSPLMWFRGYQLPDTPAIDGGYYL-------TGD 320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 465 LLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGvtVPEHE-GRVGMAAIKLKEGkkFDG-PNIY 542
Cdd:cd05973 321 TVEFDPDGSFSFIGRADDVITMSGYRIGPFDVESALIEHPAVAEAAVIG--VPDPErTEVVKAFVVLRGG--HEGtPALA 396
|
490 500 510
....*....|....*....|....*....|....*.
gi 556957071 543 K----HVMEYLPNYARPRFVRIQEAIEITGTFK-QR 573
Cdd:cd05973 397 DelqlHVKKRLSAHAYPRTIHFVDELPKTPSGKiQR 432
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
58-239 |
6.77e-14 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 74.16 E-value: 6.77e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 58 FLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNI 137
Cdd:cd12117 3 FEEQAARTPDAVAVVYGDRSLTYAELNERANRLARRL-RAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPLDPEL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 138 ---RSRAFLHSfncSGAKVLIAGADLKGAVEEVLPNLrennvtifyLNDEPITQGVESLkEKIEASSDQPipksfrittt 214
Cdd:cd12117 82 paeRLAFMLAD---AGAKVLLTDRSLAGRAGGLEVAV---------VIDEALDAGPAGN-PAVPVSPDDL---------- 138
|
170 180
....*....|....*....|....*
gi 556957071 215 tktaALYIYTSGTTGLPKAAIINQR 239
Cdd:cd12117 139 ----AYVMYTSGSTGRPKGVAVTHR 159
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
53-259 |
8.25e-14 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 74.89 E-value: 8.25e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 53 TLLDKFLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCsmac 132
Cdd:COG1020 477 TLHELFEAQAARTPDAVAVVFGDQSLTYAELNARANRLAHHL-RALGVGPGDLVGVCLERSLEMVVALLAVLKAGA---- 551
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 133 lncnirsrAFL------------HSFNCSGAKVLIAGADLkgaveevLPNLRENNVTIFYLNDEPITQgveslkekieAS 200
Cdd:COG1020 552 --------AYVpldpaypaerlaYMLEDAGARLVLTQSAL-------AARLPELGVPVLALDALALAA----------EP 606
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 556957071 201 SDQPIPksfriTTTTKTAAlY-IYTSGTTGLPKAAIINQRRLwgASFLLSIS---GVTSEDVV 259
Cdd:COG1020 607 ATNPPV-----PVTPDDLA-YvIYTSGSTGRPKGVMVEHRAL--VNLLAWMQrryGLGPGDRV 661
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
79-511 |
1.24e-13 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 73.07 E-value: 1.24e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 79 TYLEVDKRSNQVARALQREAALKEGDTAALFLGNEP-AYIWIwLGLAKLGCsmACLNCNIRS----RAFLhsFNCSGAKV 153
Cdd:TIGR01733 1 TYRELDERANRLARHLRAAGGVGPGDRVAVLLERSAeLVVAI-LAVLKAGA--AYVPLDPAYpaerLAFI--LEDAGARL 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 154 LIAGADLKG-AVEEVLPNLRENNVTIFYLNDEPITQGVESLkekieASSDQPipksfrittttktaALYIYTSGTTGLPK 232
Cdd:TIGR01733 76 LLTDSALASrLAGLVLPVILLDPLELAALDDAPAPPPPDAP-----SGPDDL--------------AYVIYTSGSTGRPK 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 233 AAIINQRRLwgASFLLSIS---GVTSEDVV--YTPL-----------PLYHSAGLLLGVNGcielgttcVLRRKFSASQF 296
Cdd:TIGR01733 137 GVVVTHRSL--VNLLAWLArryGLDPDDRVlqFASLsfdasveeifgALLAGATLVVPPED--------EERDDAALLAA 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 297 WNdcREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVrIAVGNGLRADVWKEFLNRFGKIRICEFYAATEGNIAFINYSGK 376
Cdd:TIGR01733 207 LI--AEHPVTVLNLTPSLLALLAAALPPALASLRLV-ILGGEALTPALVDRWRARGPGARLINLYGPTETTVWSTATLVD 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 377 IGAVGRANFLhKRGVPYElikfNTEKEepVRDANGHciHVSKGETGLLVakITTRSPFCGYAGSKTQSEKKILRDVFRKG 456
Cdd:TIGR01733 284 PDDAPRESPV-PIGRPLA----NTRLY--VLDDDLR--PVPVGVVGELY--IGGPGVARGYLNRPELTAERFVPDPFAGG 352
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*..
gi 556957071 457 D--IFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASV 511
Cdd:TIGR01733 353 DgaRLYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLRHPGVREAVV 409
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
77-291 |
3.27e-13 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 72.32 E-value: 3.27e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 77 VYTYLEVDKRSNQVARALQReAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKVLIA 156
Cdd:PLN02246 50 VYTYADVELLSRRVAAGLHK-LGIRQGDVVMLLLPNCPEFVLAFLGASRRGAVTTTANPFYTPAEIAKQAKASGAKLIIT 128
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 157 GAdlkGAVEEVLPNLRENNVTIFYLNDEP--------ITQGVESLKEKIEASSDQPIpksfrittttktaALyIYTSGTT 228
Cdd:PLN02246 129 QS---CYVDKLKGLAEDDGVTVVTIDDPPegclhfseLTQADENELPEVEISPDDVV-------------AL-PYSSGTT 191
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 556957071 229 GLPKAAIINQRrlwgaSFLLSIS----------GVTSEDVVYTPLPLYH----SAGLLLGvngcIELGTTCVLRRKF 291
Cdd:PLN02246 192 GLPKGVMLTHK-----GLVTSVAqqvdgenpnlYFHSDDVILCVLPMFHiyslNSVLLCG----LRVGAAILIMPKF 259
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
53-576 |
3.85e-13 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 72.32 E-value: 3.85e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 53 TLLDKFLQQVEKQPHKPFLV--FEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSM 130
Cdd:PLN02330 29 TLPDFVLQDAELYADKVAFVeaVTGKAVTYGEVVRDTRRFAKAL-RSLGLRKGQVVVVVLPNVAEYGIVALGIMAAGGVF 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 131 ACLNCNIRSRAFLHSFNCSGAKVLIAGADLKGAVEEV-LPnlrennvtIFYLNDEPITQGVeSLKEKIEASSDQPIPKSF 209
Cdd:PLN02330 108 SGANPTALESEIKKQAEAAGAKLIVTNDTNYGKVKGLgLP--------VIVLGEEKIEGAV-NWKELLEAADRAGDTSDN 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 210 RITTTTKTAALyIYTSGTTGLPKAAIINQRRLWgASFLLSISGVTSEDV--VYTP--LPLYHsaglLLGVNG--CIEL-- 281
Cdd:PLN02330 179 EEILQTDLCAL-PFSSGTTGISKGVMLTHRNLV-ANLCSSLFSVGPEMIgqVVTLglIPFFH----IYGITGicCATLrn 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 282 -GTTCVLRRkFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRI----AVGNGLRADVWKEFLNRFGKIR 356
Cdd:PLN02330 253 kGKVVVMSR-FELRTFLNALITQEVSFAPIVPPIILNLVKNPIVEEFDLSKLKLqaimTAAAPLAPELLTAFEAKFPGVQ 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 357 ICEFYAATEGNIAFINYSGKIGAVGRANflhKRGVPYELIKFNTEKEEPvrdanghcihvskgETGLLVAK-----ITTR 431
Cdd:PLN02330 332 VQEAYGLTEHSCITLTHGDPEKGHGIAK---KNSVGFILPNLEVKFIDP--------------DTGRSLPKntpgeLCVR 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 432 SPfC---GYAGSKTQSEKKIlrdvfrKGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQE 508
Cdd:PLN02330 395 SQ-CvmqGYYNNKEETDRTI------DEDGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVED 467
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 556957071 509 ASVygVTVPEHE-GRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAI--EITGTFKQRKVK 576
Cdd:PLN02330 468 AAV--VPLPDEEaGEIPAACVVINPKAKESEEDILNFVAANVAHYKKVRVVQFVDSIpkSLSGKIMRRLLK 536
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
53-579 |
9.93e-13 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 70.93 E-value: 9.93e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 53 TLLDKFLQQvekQPHKPFLVFEKQVYTYLEVDKRSNQVARALQReAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMAC 132
Cdd:PRK06164 14 SLLDAHARA---RPDAVALIDEDRPLSRAELRALVDRLAAWLAA-QGVRRGDRVAVWLPNCIEWVVLFLACARLGATVIA 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 133 LNCNIRSRAFLHSFNCSGAKVLIA-----GADLKGAVEEVLPNLRennvtifylndePITQGVESLKEkieASSDQPIPK 207
Cdd:PRK06164 90 VNTRYRSHEVAHILGRGRARWLVVwpgfkGIDFAAILAAVPPDAL------------PPLRAIAVVDD---AADATPAPA 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 208 SFRITTTTKTA------------------ALYIYTSGTTGLPKAAIINQRRL----WGASFLLsisGVTSEDVVYTPLPL 265
Cdd:PRK06164 155 PGARVQLFALPdpappaaageraadpdagALLFTTSGTTSGPKLVLHRQATLlrhaRAIARAY---GYDPGAVLLAALPF 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 266 YHSAGLLlGVNGCIELGTTCVLRRKFSASQFWNDCREYNTTAIQYIGEMLRylcstprkdndrdhkvRIAVGNGLRADvw 345
Cdd:PRK06164 232 CGVFGFS-TLLGALAGGAPLVCEPVFDAARTARALRRHRVTHTFGNDEMLR----------------RILDTAGERAD-- 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 346 keflnrFGKIRICEFyaategnIAFINYSGKIGAVGRAnflhkRGVPY-------ELIKFNT--EKEEPVRD---ANGHC 413
Cdd:PRK06164 293 ------FPSARLFGF-------ASFAPALGELAALARA-----RGVPLtglygssEVQALVAlqPATDPVSVrieGGGRP 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 414 IH------VSKGETGLLVA-----KITTRSP--FCGYAGSKTQSEKKILRDvfrkGdiFFNSGDLLMIDHDNFIYFKDRI 480
Cdd:PRK06164 355 ASpearvrARDPQDGALLPdgesgEIEIRAPslMRGYLDNPDATARALTDD----G--YFRTGDLGYTRGDGQFVYQTRM 428
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 481 GDTFRWKGENVATCEVADLLENVDFIQEASVYGVTvpeHEGR-VGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARP-RFV 558
Cdd:PRK06164 429 GDSLRLGGFLVNPAEIEHALEALPGVAAAQVVGAT---RDGKtVPVAFVIPTDGASPDEAGLMAACREALAGFKVPaRVQ 505
|
570 580
....*....|....*....|...
gi 556957071 559 RIQE--AIEITGTFKQRKVKLVE 579
Cdd:PRK06164 506 VVEAfpVTESANGAKIQKHRLRE 528
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
90-576 |
1.02e-12 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 71.03 E-value: 1.02e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 90 VARALQREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLN-----CNIRSRAFlhsfNCSGAKVLIAGADLkgav 164
Cdd:PLN02574 79 MAAGLYHVMGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNpssslGEIKKRVV----DCSVGLAFTSPENV---- 150
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 165 eEVLPNLRENNVTI---FYLNDepITQGVESLKEKIEASSDqPIPKSfrITTTTKTAALyIYTSGTTGLPKAAIINQRRL 241
Cdd:PLN02574 151 -EKLSPLGVPVIGVpenYDFDS--KRIEFPKFYELIKEDFD-FVPKP--VIKQDDVAAI-MYSSGTTGASKGVVLTHRNL 223
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 242 WGA-----SFLLSISGVTSEDVVY-TPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWNDCREYNTTAIQYIGEML 315
Cdd:PLN02574 224 IAMvelfvRFEASQYEYPGSDNVYlAALPMFHIYGLSLFVVGLLSLGSTIVVMRRFDASDMVKVIDRFKVTHFPVVPPIL 303
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 316 RYLCSTPRKDNDRDHKVRIAVGNG---LRADVWKEFLNRFGKIRICEFYAATEGNiafinysgkigAVGranflhKRGvp 392
Cdd:PLN02574 304 MALTKKAKGVCGEVLKSLKQVSCGaapLSGKFIQDFVQTLPHVDFIQGYGMTEST-----------AVG------TRG-- 364
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 393 yelikFNTEKEEpvrdanghcihvSKGETGLLV----AKITTRSPFC----GYAGSKTQSEKKILRDVFRKGDI------ 458
Cdd:PLN02574 365 -----FNTEKLS------------KYSSVGLLApnmqAKVVDWSTGCllppGNCGELWIQGPGVMKGYLNNPKAtqstid 427
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 459 ---FFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVtVPEHEGRVGMAAIKLKEGKK 535
Cdd:PLN02574 428 kdgWLRTGDIAYFDEDGYLYIVDRLKEIIKYKGFQIAPADLEAVLISHPEIIDAAVTAV-PDKECGEIPVAFVVRRQGST 506
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 556957071 536 FDGPNIYKHVMEYLPNYARPRFVRIQEAI--EITGTFKQRKVK 576
Cdd:PLN02574 507 LSQEAVINYVAKQVAPYKKVRKVVFVQSIpkSPAGKILRRELK 549
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
66-560 |
1.34e-12 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 70.25 E-value: 1.34e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 66 PHKPFLVFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCsmaclncnirsrAFL-- 143
Cdd:cd05930 1 PDAVAVVDGDQSLTYAELDARANRLARYL-RERGVGPGDLVAVLLERSLEMVVAILAVLKAGA------------AYVpl 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 144 ----------HSFNCSGAKVLIAGADlkgaveevlpnlrennvtifylndepitqgveslkekieassdqpipksfritt 213
Cdd:cd05930 68 dpsypaerlaYILEDSGAKLVLTDPD------------------------------------------------------ 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 214 ttkTAALYIYTSGTTGLPKAAIINQRRLwgASFLLSIS---GVTSEDVV--YTPLPLYHSAGLLLG--VNGcielGTTCV 286
Cdd:cd05930 94 ---DLAYVIYTSGSTGKPKGVMVEHRGL--VNLLLWMQeayPLTPGDRVlqFTSFSFDVSVWEIFGalLAG----ATLVV 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 287 LRR--KFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPrkDNDRDHKVRIAV--GNGLRADVWKEFLNRFGKIRICEFYA 362
Cdd:cd05930 165 LPEevRKDPEALADLLAEEGITVLHLTPSLLRLLLQEL--ELAALPSLRLVLvgGEALPPDLVRRWRELLPGARLVNLYG 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 363 ATEGNIAFINYsgkigaVGRANFLHKRGVPyelIK---FNTEKEepVRDANGHCihVSKGETGLLVakITTRSPFCGYAG 439
Cdd:cd05930 243 PTEATVDATYY------RVPPDDEEDGRVP---IGrpiPNTRVY--VLDENLRP--VPPGVPGELY--IGGAGLARGYLN 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 440 SKTQSEKKILRDVFRKGDIFFNSGDLLMIDHDNFIYFKDRIgDT------FRwkgenVATCEVADLLENVDFIQEASVyg 513
Cdd:cd05930 308 RPELTAERFVPNPFGPGERMYRTGDLVRWLPDGNLEFLGRI-DDqvkirgYR-----IELGEIEAALLAHPGVREAAV-- 379
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 556957071 514 vtVPEHEGRVGM---AAIKLKEGKKFDGPNIYKHVMEYLPNYARP-RFVRI 560
Cdd:cd05930 380 --VAREDGDGEKrlvAYVVPDEGGELDEEELRAHLAERLPDYMVPsAFVVL 428
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
218-560 |
1.94e-12 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 69.90 E-value: 1.94e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 218 AALyIYTSGTTGLPKAAIINQRRLWG-ASFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQF 296
Cdd:PRK07514 159 AAI-LYTSGTTGRSKGAMLSHGNLLSnALTLVDYWRFTPDDVLIHALPIFHTHGLFVATNVALLAGASMIFLPKFDPDAV 237
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 297 wndcreynttaiqyIGEMLR----------Y--LCSTPRKDNDRDHKVRIAV-GNG-LRADVWKEFLNRFGKiRICEFYA 362
Cdd:PRK07514 238 --------------LALMPRatvmmgvptfYtrLLQEPRLTREAAAHMRLFIsGSApLLAETHREFQERTGH-AILERYG 302
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 363 ATEGNIAFIN-YSGK--IGAVGRAnfLhkRGVPYELIKFNTEKEEPVrdanghcihvskGETGLlvakITTRSP--FCGY 437
Cdd:PRK07514 303 MTETNMNTSNpYDGErrAGTVGFP--L--PGVSLRVTDPETGAELPP------------GEIGM----IEVKGPnvFKGY 362
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 438 --AGSKTQSEkkilrdvFRkGDIFFNSGDLLMIDHDNFIYF----KDRI--GdtfrwkGENVATCEVADLLENVDFIQEA 509
Cdd:PRK07514 363 wrMPEKTAEE-------FR-ADGFFITGDLGKIDERGYVHIvgrgKDLIisG------GYNVYPKEVEGEIDELPGVVES 428
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|.
gi 556957071 510 SVYGVTVPEHeGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRI 560
Cdd:PRK07514 429 AVIGVPHPDF-GEGVTAVVVPKPGAALDEAAILAALKGRLARFKQPKRVFF 478
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
62-514 |
2.02e-12 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 69.92 E-value: 2.02e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 62 VEKQPHKPFLVFEKQ--VYTYLEVDKRSNQVARALQReAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLN----- 134
Cdd:PRK05852 26 ATRLPEAPALVVTADriAISYRDLARLVDDLAGQLTR-SGLLPGDRVALRMGSNAEFVVALLAASRADLVVVPLDpalpi 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 135 --CNIRSRAflhsfncSGAKVLIAgaDLKGAVEEVLPNLRENNVTIFYLNDEPITQGVES--LKEKIEASSDQPIPKSFR 210
Cdd:PRK05852 105 aeQRVRSQA-------AGARVVLI--DADGPHDRAEPTTRWWPLTVNVGGDSGPSGGTLSvhLDAATEPTPATSTPEGLR 175
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 211 ittttKTAALYIYTSGTTGLPK------AAIINQRRLWGASFLLSisgvtSEDVVYTPLPLYHSAGLLLGVNGCIELGTT 284
Cdd:PRK05852 176 -----PDDAMIMFTGGTTGLPKmvpwthANIASSVRAIITGYRLS-----PRDATVAVMPLYHGHGLIAALLATLASGGA 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 285 CVL--RRKFSASQFWNDCREYNT---TAIQYIGEMLRYLCSTPRKDNDRdHKVRI--AVGNGLRADVWKEFLNRFGKIRI 357
Cdd:PRK05852 246 VLLpaRGRFSAHTFWDDIKAVGAtwyTAVPTIHQILLERAATEPSGRKP-AALRFirSCSAPLTAETAQALQTEFAAPVV 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 358 CEFyAATEGNIAFInySGKIGAVGRAnflHKRGVPYELIKFNTEKEEPVRDANGHciHVSKGETGllvaKITTRSPFC-- 435
Cdd:PRK05852 325 CAF-GMTEATHQVT--TTQIEGIGQT---ENPVVSTGLVGRSTGAQIRIVGSDGL--PLPAGAVG----EVWLRGTTVvr 392
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 556957071 436 GYAGsktqsEKKILRDVFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGV 514
Cdd:PRK05852 393 GYLG-----DPTITAANFTDG--WLRTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASHPNVMEAAVFGV 464
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
58-577 |
4.44e-12 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 68.89 E-value: 4.44e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 58 FLQQV-EKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQrEAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCN 136
Cdd:PLN03102 19 FLKRAsECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLI-SLNITKNDVVSVLAPNTPAMYEMHFAVPMAGAVLNPINTR 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 137 IRSRAFLHSFNCSGAKVLIAGADLKGAVEEVL---------PNLRennVTIFYLNDEPITQGVESLKEKIEASSDQPIPK 207
Cdd:PLN03102 98 LDATSIAAILRHAKPKILFVDRSFEPLAREVLhllssedsnLNLP---VIFIHEIDFPKRPSSEELDYECLIQRGEPTPS 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 208 S----FRITTTTKTAALYiYTSGTTGLPKAAIINQRrlwgASFLLSISGVTSEDVVYTP-----LPLYHSAGLLLgVNGC 278
Cdd:PLN03102 175 LvarmFRIQDEHDPISLN-YTSGTTADPKGVVISHR----GAYLSTLSAIIGWEMGTCPvylwtLPMFHCNGWTF-TWGT 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 279 IELGTTCVLRRKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKD-NDRDHKVRIAVGNGLRADVWKEFLNRFGkIRI 357
Cdd:PLN03102 249 AARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDlSPRSGPVHVLTGGSPPPAALVKKVQRLG-FQV 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 358 CEFYAATE--GNIAFINYS---GKIGAVGRANFLHKRGVPY----ELIKFNTEKEEPV-RDANGHCIHVSKGETgllVAK 427
Cdd:PLN03102 328 MHAYGLTEatGPVLFCEWQdewNRLPENQQMELKARQGVSIlglaDVDVKNKETQESVpRDGKTMGEIVIKGSS---IMK 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 428 ITTRSPfcgyagsKTQSEkkilrdVFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQ 507
Cdd:PLN03102 405 GYLKNP-------KATSE------AFKHG--WLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVL 469
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 508 EASVYGVTVPEHeGRVGMAAIKLKEGKKFDGP----------NIYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKL 577
Cdd:PLN03102 470 ETAVVAMPHPTW-GETPCAFVVLEKGETTKEDrvdklvtrerDLIEYCRENLPHFMCPRKVVFLQELPKNGNGKILKPKL 548
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
77-513 |
7.64e-12 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 67.79 E-value: 7.64e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 77 VYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKVLIA 156
Cdd:cd05903 1 RLTYSELDTRADRLAAGL-AALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVV 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 157 GADLKGaveevlpnlrennvtifylndepitqgveslkEKIEASSDQPipksfrittttktaALYIYTSGTTGLPKAAII 236
Cdd:cd05903 80 PERFRQ--------------------------------FDPAAMPDAV--------------ALLLFTSGTTGEPKGVMH 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 237 NQRRL-WGASFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWNDCREYNTTAIQYIGEML 315
Cdd:cd05903 114 SHNTLsASIRQYAERLGLGPGDVFLVASPMAHQTGFVYGFTLPLLLGAPVVLQDIWDPDKALALMREHGVTFMMGATPFL 193
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 316 RYLCSTPRKDNDR--DHKVRIAVGNGLRADVWKEFLNRFGKIrICEFYAATEGNiafinysgkiGAVGRAN------FLH 387
Cdd:cd05903 194 TDLLNAVEEAGEPlsRLRTFVCGGATVPRSLARRAAELLGAK-VCSAYGSTECP----------GAVTSITpapedrRLY 262
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 388 KRGVPYELIkfntekEEPVRDANGHCihVSKGETGLLVAkittRSP--FCGYAgsktqSEKKILRDVFRKGdiFFNSGDL 465
Cdd:cd05903 263 TDGRPLPGV------EIKVVDDTGAT--LAPGVEGELLS----RGPsvFLGYL-----DRPDLTADAAPEG--WFRTGDL 323
|
410 420 430 440
....*....|....*....|....*....|....*....|....*...
gi 556957071 466 LMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYG 513
Cdd:cd05903 324 ARLDEDGYLRITGRSKDIIIRGGENIPVLEVEDLLLGHPGVIEAAVVA 371
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
39-579 |
1.87e-11 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 66.75 E-value: 1.87e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 39 NYKMKKFEEKEPVFTLLDKFlqqVEKQPHKPFLVF-----EKQVYTYLEVDKRSNQVARALQREAaLKEGDTAALFLGNE 113
Cdd:cd05970 7 NFSINVPENFNFAYDVVDAM---AKEYPDKLALVWcddagEERIFTFAELADYSDKTANFFKAMG-IGKGDTVMLTLKRR 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 114 PAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAK--VLIAGADLKGAVEEVLPNLreNNVTIFYLNDEPITQGVE 191
Cdd:cd05970 83 YEFWYSLLALHKLGAIAIPATHQLTAKDIVYRIESADIKmiVAIAEDNIPEEIEKAAPEC--PSKPKLVWVGDPVPEGWI 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 192 SLKEKIEASSDQPIPKSFRITTTTKTAALYIYTSGTTGLPKaaIINQRRLWGASFLLSIS---GVTSEDVVYTplplYHS 268
Cdd:cd05970 161 DFRKLIKNASPDFERPTANSYPCGEDILLVYFSSGTTGMPK--MVEHDFTYPLGHIVTAKywqNVREGGLHLT----VAD 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 269 AGLLLGVNGCIE---LGTTCVL---RRKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDhKVRIAV--GNGL 340
Cdd:cd05970 235 TGWGKAVWGKIYgqwIAGAAVFvydYDKFDPKALLEKLSKYGVTTFCAPPTIYRFLIREDLSRYDLS-SLRYCTtaGEAL 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 341 RADVWKEFLNRFGkIRICEFYAATEGNIAFINYSG---KIGAVGRANflhkRGVPYELIkfntekeepvrDANGHCIHVs 417
Cdd:cd05970 314 NPEVFNTFKEKTG-IKLMEGFGQTETTLTIATFPWmepKPGSMGKPA----PGYEIDLI-----------DREGRSCEA- 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 418 kGETGLLVAKITTRSPFCGYAGSKTQSEKKilRDVFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVA 497
Cdd:cd05970 377 -GEEGEIVIRTSKGKPVGLFGGYYKDAEKT--AEVWHDG--YYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVE 451
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 498 DLLENVDFIQEASVYGVTVPEhEGRVGMAAIKLKEGKKfDGPNIYKHVMEYLPN----YARPRFVRIQEAIEITGTFKQR 573
Cdd:cd05970 452 SALIQHPAVLECAVTGVPDPI-RGQVVKATIVLAKGYE-PSEELKKELQDHVKKvtapYKYPRIVEFVDELPKTISGKIR 529
|
....*.
gi 556957071 574 KVKLVE 579
Cdd:cd05970 530 RVEIRE 535
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
53-580 |
2.13e-11 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 66.62 E-value: 2.13e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 53 TLLDKFLQQVEKQPHKPFLV------FEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEpayiWIWLGLAkL 126
Cdd:PRK13295 25 TINDDLDACVASCPDKTAVTavrlgtGAPRRFTYRELAALVDRVAVGL-ARLGVGRGDVVSCQLPNW----WEFTVLY-L 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 127 GCSM--ACLNCNI---RSRAFLHSFNCSGAKVLIA-----GADLKGAVEEV---LPNLREnnvtIFYLNDEpitqGVESL 193
Cdd:PRK13295 99 ACSRigAVLNPLMpifRERELSFMLKHAESKVLVVpktfrGFDHAAMARRLrpeLPALRH----VVVVGGD----GADSF 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 194 K------EKIEASSDQPIPKSFRitTTTKTAALYIYTSGTTGLPKAAIINQRRLWgaSFLLSIS---GVTSEDVVYTPLP 264
Cdd:PRK13295 171 EallitpAWEQEPDAPAILARLR--PGPDDVTQLIYTSGTTGEPKGVMHTANTLM--ANIVPYAerlGLGADDVILMASP 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 265 LYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRI------AVGN 338
Cdd:PRK13295 247 MAHQTGFMYGLMMPVMLGATAVLQDIWDPARAAELIRTEGVTFTMASTPFLTDLTRAVKESGRPVSSLRTflcagaPIPG 326
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 339 GLRADVWKeflnRFGkIRICEFYAATEGniafinysgkiGAVGranfLHKRGVPYELIkFNTE------KEEPVRDANGH 412
Cdd:PRK13295 327 ALVERARA----ALG-AKIVSAWGMTEN-----------GAVT----LTKLDDPDERA-STTDgcplpgVEVRVVDADGA 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 413 ciHVSKGETGLLVAkittRSP--FCGYAGsktqseKKILRDVFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGEN 490
Cdd:PRK13295 386 --PLPAGQIGRLQV----RGCsnFGGYLK------RPQLNGTDADG--WFDTGDLARIDADGYIRISGRSKDVIIRGGEN 451
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 491 VATCEVADLLENVDFIQEASVygVTVP-EHEGRVGMAAIKLKEGKKFDGPNI------YKHVMEYLPNyarpRFVrIQEA 563
Cdd:PRK13295 452 IPVVEIEALLYRHPAIAQVAI--VAYPdERLGERACAFVVPRPGQSLDFEEMveflkaQKVAKQYIPE----RLV-VRDA 524
|
570
....*....|....*..
gi 556957071 564 IEITGTFKQRKVKLVEE 580
Cdd:PRK13295 525 LPRTPSGKIQKFRLREM 541
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
51-547 |
8.24e-11 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 64.82 E-value: 8.24e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 51 VFTLLDKFLQQvekQPHKPFLVFEKQ-----VYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAK 125
Cdd:cd05968 63 VEQLLDKWLAD---TRTRPALRWEGEdgtsrTLTYGELLYEVKRLANGL-RALGVGKGDRVGIYLPMIPEIVPAFLAVAR 138
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 126 LGCSMACLNCNIRSRAFLHSFNCSGAKVLIAgADL---KGAVEEVLPNLRENNVTIFYL----------NDEPITQGVES 192
Cdd:cd05968 139 IGGIVVPIFSGFGKEAAATRLQDAEAKALIT-ADGftrRGREVNLKEEADKACAQCPTVekvvvvrhlgNDFTPAKGRDL 217
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 193 LKEKIEASSDQPIPKsfritTTTKTAALYIYTSGTTGLPKAAIINQR--RLWGASFLLSISGVTSEDVVY--TPLplyhs 268
Cdd:cd05968 218 SYDEEKETAGDGAER-----TESEDPLMIIYTSGTTGKPKGTVHVHAgfPLKAAQDMYFQFDLKPGDLLTwfTDL----- 287
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 269 aGLLLG---VNGCIELGTTCVLRRKF----SASQFWNDCREYNTTAIQYIGEMLRYL---CSTPRKDNDRDhKVRIAVGN 338
Cdd:cd05968 288 -GWMMGpwlIFGGLILGATMVLYDGApdhpKADRLWRMVEDHEITHLGLSPTLIRALkprGDAPVNAHDLS-SLRVLGST 365
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 339 G--LRADVWKEFLNRFGKiricefyaateGNIAFINYSGKI----GAVGranflhkrGVPYELIK---FNTE---KEEPV 406
Cdd:cd05968 366 GepWNPEPWNWLFETVGK-----------GRNPIINYSGGTeisgGILG--------NVLIKPIKpssFNGPvpgMKADV 426
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 407 RDANGHCIHVSKGETGLLvakittrSPFCGYAGSKTQSEKKILRDVFRKGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRW 486
Cdd:cd05968 427 LDESGKPARPEVGELVLL-------APWPGMTRGFWRDEDRYLETYWSRFDNVWVHGDFAYYDEEGYFYILGRSDDTINV 499
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 556957071 487 KGENVATCEVADLLENVDFIQEASVYGVTVPEhEGRVGMAAIKLKEGKKFdGPNIYKHVME 547
Cdd:cd05968 500 AGKRVGPAEIESVLNAHPAVLESAAIGVPHPV-KGEAIVCFVVLKPGVTP-TEALAEELME 558
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
74-235 |
1.55e-10 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 63.76 E-value: 1.55e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 74 EKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACL-----NCNIRSRaflhsFNC 148
Cdd:PRK04319 70 RKEKYTYKELKELSNKFANVL-KELGVEKGDRVFIFMPRIPELYFALLGALKNGAIVGPLfeafmEEAVRDR-----LED 143
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 149 SGAKVLIAGADL---KGAVEevLPNLRennvTIFyLNDEPITQ--GVESLKEKIEASSDQpipksFRITTTTKT-AALYI 222
Cdd:PRK04319 144 SEAKVLITTPALlerKPADD--LPSLK----HVL-LVGEDVEEgpGTLDFNALMEQASDE-----FDIEWTDREdGAILH 211
|
170
....*....|...
gi 556957071 223 YTSGTTGLPKAAI 235
Cdd:PRK04319 212 YTSGSTGKPKGVL 224
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
495-571 |
2.50e-10 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 56.78 E-value: 2.50e-10
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 556957071 495 EVADLLENVDFIQEASVYGVTVPEhEGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFK 571
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDEL-KGEAPVAFVVLKPGVELLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
218-574 |
4.71e-10 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 62.51 E-value: 4.71e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 218 AALYIYTSGTTGLPKAAIINQRRLWGASFL-LSISGVTSEDVVYTPLPLYHSAGL-----LLGVNGCielgttCVLRRKF 291
Cdd:PLN02860 174 AVLICFTSGTTGRPKGVTISHSALIVQSLAkIAIVGYGEDDVYLHTAPLCHIGGLssalaMLMVGAC------HVLLPKF 247
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 292 SASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRD--HKVRIAV--GNGLRADVWKEFLNRFGKIRICEFYAATEG- 366
Cdd:PLN02860 248 DAKAALQAIKQHNVTSMITVPAMMADLISLTRKSMTWKvfPSVRKILngGGSLSSRLLPDAKKLFPNAKLFSAYGMTEAc 327
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 367 -NIAFInysgkigavgRANFLHKRGVPYELIKFNTEKEEPVRDANGHCI-----HVS---KGETGLLVAKITTRSP--FC 435
Cdd:PLN02860 328 sSLTFM----------TLHDPTLESPKQTLQTVNQTKSSSVHQPQGVCVgkpapHVElkiGLDESSRVGRILTRGPhvML 397
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 436 GYAG--SKTQSEkkilrdvfRKGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVadllENVdFIQEASVYG 513
Cdd:PLN02860 398 GYWGqnSETASV--------LSNDGWLDTGDIGWIDKAGNLWLIGRSNDRIKTGGENVYPEEV----EAV-LSQHPGVAS 464
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 514 VTV---PEHE-GRVGMAAIKLKEGKKF---DGPNiYKHVM----EYLPNYAR---------PR-FVRIQEAIEITGTFKQ 572
Cdd:PLN02860 465 VVVvgvPDSRlTEMVVACVRLRDGWIWsdnEKEN-AKKNLtlssETLRHHCReknlsrfkiPKlFVQWRKPFPLTTTGKI 543
|
..
gi 556957071 573 RK 574
Cdd:PLN02860 544 RR 545
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
59-568 |
5.41e-10 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 61.93 E-value: 5.41e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 59 LQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAyIWIWLGLAKL-GCSMACLNcni 137
Cdd:PRK06188 19 VSALKRYPDRPALVLGDTRLTYGQLADRISRYIQAF-EALGLGTGDAVALLSLNRPE-VLMAIGAAQLaGLRRTALH--- 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 138 rSRAFL--HSFNC--SGAKVLI----AGADLKGAVEEVLPNLREnnvtIFYLNDEPITQGVESLKEKIEassdqpiPKSF 209
Cdd:PRK06188 94 -PLGSLddHAYVLedAGISTLIvdpaPFVERALALLARVPSLKH----VLTLGPVPDGVDLLAAAAKFG-------PAPL 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 210 RITTTTKTAALYIYTSGTTGLPKAAIINQRRLWGASFLLSISGVTSEDVVY-TPLPLYHSAGLLlgVNGCIELGTTCVLR 288
Cdd:PRK06188 162 VAAALPPDIAGLAYTGGTTGKPKGVMGTHRSIATMAQIQLAEWEWPADPRFlMCTPLSHAGGAF--FLPTLLRGGTVIVL 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 289 RKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRkDNDRD--------------HKVRIAvgnglradvwkEFLNRFGK 354
Cdd:PRK06188 240 AKFDPAEVLRAIEEQRITATFLVPTMIYALLDHPD-LRTRDlssletvyygaspmSPVRLA-----------EAIERFGP 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 355 IrICEFYAATEGNIAfINYSGKIGavgranflHKRGVPYEL------IKFNTEKeepVRDANGHciHVSKGETGllvaKI 428
Cdd:PRK06188 308 I-FAQYYGQTEAPMV-ITYLRKRD--------HDPDDPKRLtscgrpTPGLRVA---LLDEDGR--EVAQGEVG----EI 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 429 TTRSPFC--GYAGSKTQSEKkilrdVFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFI 506
Cdd:PRK06188 369 CVRGPLVmdGYWNRPEETAE-----AFRDG--WLHTGDVAREDEDGFYYIVDRKKDMIVTGGFNVFPREVEDVLAEHPAV 441
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 556957071 507 QEASVYGvtVP-EHEGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITG 568
Cdd:PRK06188 442 AQVAVIG--VPdEKWGEAVTAVVVLRPGAAVDAAELQAHVKERKGSVHAPKQVDFVDSLPLTA 502
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
78-513 |
5.46e-10 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 61.99 E-value: 5.46e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 78 YTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKVLIag 157
Cdd:cd17640 6 ITYKDLYQEILDFAAGL-RSLGVKAGEKVALFADNSPRWLIADQGIMALGAVDVVRGSDSSVEELLYILNHSESVALV-- 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 158 adlkgaveevlpnlrennvtifylndepitqgveslkekIEASSDQpipksfrittttkTAALyIYTSGTTGLPKAAIIN 237
Cdd:cd17640 83 ---------------------------------------VENDSDD-------------LATI-IYTSGTTGNPKGVMLT 109
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 238 QRRL-WGASFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNgCIELGTTCVLRrkfSASQFWNDCREYNTTaiqyigemlr 316
Cdd:cd17640 110 HANLlHQIRSLSDIVPPQPGDRFLSILPIWHSYERSAEYF-IFACGCSQAYT---SIRTLKDDLKRVKPH---------- 175
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 317 YLCSTPR---------KDNDRDH---------------KVRIAV-GNGLRADVWKEFLNRFGkIRICEFYAATE-GNIAF 370
Cdd:cd17640 176 YIVSVPRlweslysgiQKQVSKSspikqflflfflsggIFKFGIsGGGALPPHVDTFFEAIG-IEVLNGYGLTEtSPVVS 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 371 INYSGK--IGAVGRanflhkrgvpyelIKFNTEKEepVRDANGHCIhVSKGETGLLVAkittRSP--FCGYAGSKTQSeK 446
Cdd:cd17640 255 ARRLKCnvRGSVGR-------------PLPGTEIK--IVDPEGNVV-LPPGEKGIVWV----RGPqvMKGYYKNPEAT-S 313
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 556957071 447 KILRDvfrkgDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWK-GENVATCEVADLLENVDFIQEASVYG 513
Cdd:cd17640 314 KVLDS-----DGWFNTGDLGWLTCGGELVLTGRAKDTIVLSnGENVEPQPIEEALMRSPFIEQIMVVG 376
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
60-287 |
7.49e-10 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 61.87 E-value: 7.49e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 60 QQVEKQPHKPFLVF------EKQVYTYLEVDKRSNQVARALQREAalKEGDTAALFLGNEPAYIwiwlgLAKLGCSMACL 133
Cdd:cd05931 1 RRAAARPDRPAYTFlddeggREETLTYAELDRRARAIAARLQAVG--KPGDRVLLLAPPGLDFV-----AAFLGCLYAGA 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 134 ncnI--------------RSRAFLHSfncSGAKVLIAGADLKGAVEEVLPNLRENnvtifylnDEPITQGVESLKEKIEA 199
Cdd:cd05931 74 ---IavplppptpgrhaeRLAAILAD---AGPRVVLTTAAALAAVRAFAASRPAA--------GTPRLLVVDLLPDTSAA 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 200 SSDQPIPKSfrittttKTAALYIYTSGTTGLPKAAIINQRRLWgASFLLSIS--GVTSEDVVYTPLPLYHSAGLLLGVNG 277
Cdd:cd05931 140 DWPPPSPDP-------DDIAYLQYTSGSTGTPKGVVVTHRNLL-ANVRQIRRayGLDPGDVVVSWLPLYHDMGLIGGLLT 211
|
250
....*....|
gi 556957071 278 CIELGTTCVL 287
Cdd:cd05931 212 PLYSGGPSVL 221
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
219-571 |
8.04e-10 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 60.81 E-value: 8.04e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 219 ALYIYTSGTTGLPKAAIIN-QRRLWGASFLLSISGVTSEDVVYTPLPLYHSAGLLLgVNGCIELGTTCVLRRKFSASQfw 297
Cdd:cd17630 3 ATVILTSGSTGTPKAVVHTaANLLASAAGLHSRLGFGGGDSWLLSLPLYHVGGLAI-LVRSLLAGAELVLLERNQALA-- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 298 NDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNglrADVWKEFLNRFGK--IRICEFYAATE--GNIAFINY 373
Cdd:cd17630 80 EDLAPPGVTHVSLVPTQLQRLLDSGQGPAALKSLRAVLLGG---APIPPELLERAADrgIPLYTTYGMTEtaSQVATKRP 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 374 SG-KIGAVGRAnFLHKRgvpyelikfntekeepVRDANGHCIHVSkgetgllvakitTRSPFCGYAgsktqseKKILRDV 452
Cdd:cd17630 157 DGfGRGGVGVL-LPGRE----------------LRIVEDGEIWVG------------GASLAMGYL-------RGQLVPE 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 453 FRKGDIfFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHeGRVGMAAIklke 532
Cdd:cd17630 201 FNEDGW-FTTKDLGELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEEL-GQRPVAVI---- 274
|
330 340 350 360
....*....|....*....|....*....|....*....|...
gi 556957071 533 gkKFDGPNIYKHVMEY----LPNYARPRFVRIQEAIEITGTFK 571
Cdd:cd17630 275 --VGRGPADPAELRAWlkdkLARFKLPKRIYPVPELPRTGGGK 315
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
218-292 |
2.07e-09 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 60.00 E-value: 2.07e-09
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 556957071 218 AALYIYTSGTTGLPKAAIINQRRLwgASFLLSIS---GVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFS 292
Cdd:PRK07787 130 PALIVYTSGTTGPPKGVVLSRRAI--AADLDALAeawQWTADDVLVHGLPLFHVHGLVLGVLGPLRIGNRFVHTGRPT 205
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
58-555 |
3.36e-09 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 60.36 E-value: 3.36e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 58 FLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNI 137
Cdd:PRK12316 517 FEEQVERTPEAPALAFGEETLDYAELNRRANRLAHAL-IERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLDPEY 595
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 138 RSRAFLHSFNCSGAKVLIAgadlKGAVEEVLPNLRENNVTIFylnDEPITQgveslkekIEASSDQPiPKsfrITTTTKT 217
Cdd:PRK12316 596 PAERLAYMLEDSGVQLLLS----QSHLGRKLPLAAGVQVLDL---DRPAAW--------LEGYSEEN-PG---TELNPEN 656
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 218 AALYIYTSGTTGLPKAAIINQRRL-----W---------GASFLLSISgvTSEDVVYTPL--PLYHSAGLLLGVNGciel 281
Cdd:PRK12316 657 LAYVIYTSGSTGKPKGAGNRHRALsnrlcWmqqayglgvGDTVLQKTP--FSFDVSVWEFfwPLMSGARLVVAAPG---- 730
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 282 gttcvlrRKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNGLRADVWKEFLNRFGKIRICEFY 361
Cdd:PRK12316 731 -------DHRDPAKLVELINREGVDTLHFVPSMLQAFLQDEDVASCTSLRRIVCSGEALPADAQEQVFAKLPQAGLYNLY 803
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 362 AATEGNIAfINYSGKIGAVGRAnflhkrgVPYELIKFNTEKEepVRDANGHCIHVS-KGETGLLVAKITTrspfcGYAGS 440
Cdd:PRK12316 804 GPTEAAID-VTHWTCVEEGGDS-------VPIGRPIANLACY--ILDANLEPVPVGvLGELYLAGRGLAR-----GYHGR 868
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 441 KTQSEKKILRDVFRKGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVY-------- 512
Cdd:PRK12316 869 PGLTAERFVPSPFVAGERMYRTGDLARYRADGVIEYAGRIDHQVKLRGLRIELGEIEARLLEHPWVREAAVLavdgkqlv 948
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 556957071 513 GVTVPEHEGRVGMAAIKlkegkkfdgpniyKHVMEYLPNYARP 555
Cdd:PRK12316 949 GYVVLESEGGDWREALK-------------AHLAASLPEYMVP 978
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
78-517 |
7.09e-09 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 58.63 E-value: 7.09e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 78 YTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIW----IWLGlaklGCSMACLNCNIRSRAFLHSFNCSGAKV 153
Cdd:cd05932 7 FTWGEVADKARRLAAAL-RALGLEPGSKIALISKNCAEWFItdlaIWMA----GHISVPLYPTLNPDTIRYVLEHSESKA 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 154 LIAG-----ADLKGAVEEVLPNlrennVTIFYLNDEPITQGVESLKEKIEASSDQPIPKSFRITTTtktaalyIYTSGTT 228
Cdd:cd05932 82 LFVGklddwKAMAPGVPEGLIS-----ISLPPPSAANCQYQWDDLIAQHPPLEERPTRFPEQLATL-------IYTSGTT 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 229 GLPKAAIINQRRL-WGASFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTC------------VLRRK----F 291
Cdd:cd05932 150 GQPKGVMLTFGSFaWAAQAGIEHIGTEENDRMLSYLPLAHVTERVFVEGGSLYGGVLVafaesldtfvedVQRARptlfF 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 292 SASQFWNdcrEYNTTAIQYIG-EMLRYLCSTPRKDNDRDHKVRIAVG-NGLR------ADVWKEFLNRFGKI--RICEFY 361
Cdd:cd05932 230 SVPRLWT---KFQQGVQDKIPqQKLNLLLKIPVVNSLVKRKVLKGLGlDQCRlagcgsAPVPPALLEWYRSLglNILEAY 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 362 AATEgNIAF--INYSG--KIGAVGRAnflhkrgvpyelikfntekeepvrdanGHCIHVSKGETGllvaKITTRSP--FC 435
Cdd:cd05932 307 GMTE-NFAYshLNYPGrdKIGTVGNA---------------------------GPGVEVRISEDG----EILVRSPalMM 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 436 GYAGSKTQSekkilRDVFRKgDIFFNSGDLLMIDHDNFIYFKDRIGDTFRW-KGENVATCEVADLLENVDFIQEASVYGV 514
Cdd:cd05932 355 GYYKDPEAT-----AEAFTA-DGFLRTGDKGELDADGNLTITGRVKDIFKTsKGKYVAPAPIENKLAEHDRVEMVCVIGS 428
|
...
gi 556957071 515 TVP 517
Cdd:cd05932 429 GLP 431
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
65-558 |
1.61e-08 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 57.55 E-value: 1.61e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 65 QPHKPFLVFEKQVYTYLEVDKRSNQVARALQREAaLKEGDTAALFLGNEPAyiwiwLGLAKLGCSM--ACLNC-NIRSRA 141
Cdd:PLN02479 33 HPTRKSVVHGSVRYTWAQTYQRCRRLASALAKRS-IGPGSTVAVIAPNIPA-----MYEAHFGVPMagAVVNCvNIRLNA 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 142 FLHSF--NCSGAKVLIAGADLKGAVEEVLPNLRENNVTIFylnDEPI-------TQGVESLK----------EKIEASSD 202
Cdd:PLN02479 107 PTIAFllEHSKSEVVMVDQEFFTLAEEALKILAEKKKSSF---KPPLlivigdpTCDPKSLQyalgkgaieyEKFLETGD 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 203 -----QPiPKSfrittTTKTAALYiYTSGTTGLPKAAIINQRrlwGAsFLLSISGV----TSEDVVYT-PLPLYHSAG-- 270
Cdd:PLN02479 184 pefawKP-PAD-----EWQSIALG-YTSGTTASPKGVVLHHR---GA-YLMALSNAliwgMNEGAVYLwTLPMFHCNGwc 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 271 ------LLLGVNGCIelgttcvlrRKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDN--DRDHKVRIAVGNGLRA 342
Cdd:PLN02479 253 ftwtlaALCGTNICL---------RQVTAKAIYSAIANYGVTHFCAAPVVLNTIVNAPKSETilPLPRVVHVMTAGAAPP 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 343 DVWKEFLNRFGkIRICEFYAATEGN-----IAFINYSGKIGAVGRANFLHKRGVPY----ELIKFNTEKEEPVrDANGHC 413
Cdd:PLN02479 324 PSVLFAMSEKG-FRVTHTYGLSETYgpstvCAWKPEWDSLPPEEQARLNARQGVRYigleGLDVVDTKTMKPV-PADGKT 401
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 414 IhvskGET---GLLVAKITTRSPfcgyagsktqsekKILRDVFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGEN 490
Cdd:PLN02479 402 M----GEIvmrGNMVMKGYLKNP-------------KANEEAFANG--WFHSGDLGVKHPDGYIEIKDRSKDIIISGGEN 462
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 556957071 491 VATCEVADLLENVDFIQEASVygVTVP-EHEGRVGMAAIKLKEG-KKFDGPNIYKHVMEY----LPNYARPRFV 558
Cdd:PLN02479 463 ISSLEVENVVYTHPAVLEASV--VARPdERWGESPCAFVTLKPGvDKSDEAALAEDIMKFcrerLPAYWVPKSV 534
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
57-571 |
1.69e-08 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 58.04 E-value: 1.69e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 57 KFLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCN 136
Cdd:PRK12316 2008 RIAEQAARAPEAIAVVFGDQHLSYAELDSRANRLAHRL-RARGVGPEVRVAIAAERSFELVVALLAVLKAGGAYVPLDPN 2086
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 137 IRSRAFLHSFNCSGAKVLIAGADLkgaveevlpnlrennvtifyLNDEPITQGVESLK-EKIEASSDQPIpKSFRITTTT 215
Cdd:PRK12316 2087 YPAERLAYMLEDSGAALLLTQRHL--------------------LERLPLPAGVARLPlDRDAEWADYPD-TAPAVQLAG 2145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 216 KTAALYIYTSGTTGLPKAAIINQRRLwgASFLLSI---SGVTSEDVV--YTPL-----------PLYHSAGLLLGVNGci 279
Cdd:PRK12316 2146 ENLAYVIYTSGSTGLPKGVAVSHGAL--VAHCQAAgerYELSPADCElqFMSFsfdgaheqwfhPLLNGARVLIRDDE-- 2221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 280 elgttcvlrrKFSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDhKVRIAV--GNGLRADVWKEFLNRFGKIRI 357
Cdd:PRK12316 2222 ----------LWDPEQLYDEMERHGVTILDFPPVYLQQLAEHAERDGRPP-AVRVYCfgGEAVPAASLRLAWEALRPVYL 2290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 358 CEFYAATEGNIAFINY-------SGKIGA-VGRA---NFLHKRGVPYELIkfntekeePVRDANGHCIhvskGETGLlvA 426
Cdd:PRK12316 2291 FNGYGPTEAVVTPLLWkcrpqdpCGAAYVpIGRAlgnRRAYILDADLNLL--------APGMAGELYL----GGEGL--A 2356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 427 KittrspfcGYAGSKTQSEKKILRDVF-RKGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDF 505
Cdd:PRK12316 2357 R--------GYLNRPGLTAERFVPDPFsASGERLYRTGDLARYRADGVVEYLGRIDHQVKIRGFRIELGEIEARLQAHPA 2428
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 556957071 506 IQEASVygVTVPEHEGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFK 571
Cdd:PRK12316 2429 VREAVV--VAQDGASGKQLVAYVVPDDAAEDLLAELRAWLAARLPAYMVPAHWVVLERLPLNPNGK 2492
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
78-534 |
3.28e-08 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 56.29 E-value: 3.28e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 78 YTYLEVDKRSNQVARALQrEAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKVLIAG 157
Cdd:cd05915 25 TTYAEVYQRARRLMGGLR-ALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLHTANPRLSPKEIAYILNHAEDKVLLFD 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 158 ADLKGAVEEVLPNLreNNVTifylnDEPITQgveSLKEKIE--ASSDQPIPKSFRITTTTKTAALYiYTSGTTGLPKAAI 235
Cdd:cd05915 104 PNLLPLVEAIRGEL--KTVQ-----HFVVMD---EKAPEGYlaYEEALGEEADPVRVPERAACGMA-YTTGTTGLPKGVV 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 236 INQRrlwGASFLLSISGVTSE------DVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSASQFWNDCREYNTTAIQ 309
Cdd:cd05915 173 YSHR---ALVLHSLAASLVDGtalsekDVVLPVVPMFHVNAWCLPYAATLVGAKQVLPGPRLDPASLVELFDGEGVTFTA 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 310 YIGEMLRYLCstprkdNDRDH-------KVRIAVGNGLRADVWKEfLNRFGKIRICEFYAATEgniafinysgkIGAVGR 382
Cdd:cd05915 250 GVPTVWLALA------DYLEStghrlktLRRLVVGGSAAPRSLIA-RFERMGVEVRQGYGLTE-----------TSPVVV 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 383 ANFLHKRgvpYELIKFNTEKEEPVRDANGH-----------CIHVSKGETGLLVAKITTRSPFCGYAGSKTQSEKkilrD 451
Cdd:cd05915 312 QNFVKSH---LESLSEEEKLTLKAKTGLPIplvrlrvadeeGRPVPKDGKALGEVQLKGPWITGGYYGNEEATRS----A 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 452 VFRKGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGvTVPEHEGRVGMAAIKLK 531
Cdd:cd05915 385 LTPDG--FFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVA-IPHPKWQERPLAVVVPR 461
|
...
gi 556957071 532 EGK 534
Cdd:cd05915 462 GEK 464
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
53-275 |
4.21e-08 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 55.92 E-value: 4.21e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 53 TLLDKFLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQReAALKEGDTAALFLGNEPAYIWIWLGLAKLGcsMAC 132
Cdd:COG1021 26 TLGDLLRRRAERHPDRIAVVDGERRLSYAELDRRADRLAAGLLA-LGLRPGDRVVVQLPNVAEFVIVFFALFRAG--AIP 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 133 LNC--NIRSRAFLHSFNCSGAKVLIA-----GADLKGAVEEV---LPNLRennvTIFYLNDepiTQGVESLKEKIEASSD 202
Cdd:COG1021 103 VFAlpAHRRAEISHFAEQSEAVAYIIpdrhrGFDYRALARELqaeVPSLR----HVLVVGD---AGEFTSLDALLAAPAD 175
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 203 QPIPksfriTTTTKTAALYIYTSGTTGLPKaaiinqrrL-------WGASFLLS--ISGVTSEDVVYTPLPLYHSAGL-- 271
Cdd:COG1021 176 LSEP-----RPDPDDVAFFQLSGGTTGLPK--------LiprthddYLYSVRASaeICGLDADTVYLAALPAAHNFPLss 242
|
....*.
gi 556957071 272 --LLGV 275
Cdd:COG1021 243 pgVLGV 248
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
76-575 |
7.07e-08 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 55.29 E-value: 7.07e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 76 QVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIwlglaklgcSMACLNCnirsraflhsfncsgakvli 155
Cdd:cd05907 4 QPITWAEFAEEVRALAKGL-IALGVEPGDRVAILSRNRPEWTIA---------DLAILAI-------------------- 53
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 156 agadlkGAVeevlpnlrenNVTIFY-LNDEPITQGVESLKEKI---EASSDqpipksfrittttkTAALyIYTSGTTGLP 231
Cdd:cd05907 54 ------GAV----------PVPIYPtSSAEQIAYILNDSEAKAlfvEDPDD--------------LATI-IYTSGTTGRP 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 232 KAAIINQRRL-WGASFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELG-TTCVLR---------RKFSASQF---- 296
Cdd:cd05907 103 KGVMLSHRNIlSNALALAERLPATEGDRHLSFLPLAHVFERRAGLYVPLLAGaRIYFASsaetllddlSEVRPTVFlavp 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 297 --WNdcREYNTTAIQYIGEMLRYL---CSTPRkdndrdhkVRIAVGNG--LRADVwKEFLNRFGkIRICEFYAATE-GNI 368
Cdd:cd05907 183 rvWE--KVYAAIKVKAVPGLKRKLfdlAVGGR--------LRFAASGGapLPAEL-LHFFRALG-IPVYEGYGLTEtSAV 250
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 369 AFINYSG--KIGAVGRAnflhkrgvpyelikfntekeepvrdanGHCIHVSKGETGLLVAKitTRSPFCGYAGSKTQSEK 446
Cdd:cd05907 251 VTLNPPGdnRIGTVGKP---------------------------LPGVEVRIADDGEILVR--GPNVMLGYYKNPEATAE 301
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 447 KILRDvfrkGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRW-KGENVATCEVADLLENVDFIQEASVYG--------VTVP 517
Cdd:cd05907 302 ALDAD----G--WLHTGDLGEIDEDGFLHITGRKKDLIITsGGKNISPEPIENALKASPLISQAVVIGdgrpflvaLIVP 375
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 556957071 518 EHEGRVGMAAIKLKEGKKFDG----PNIYKHVMEY-------LPNYARPRFVRI------QEAIEITGTFK-QRKV 575
Cdd:cd05907 376 DPEALEAWAEEHGIAYTDVAElaanPAVRAEIEAAveaanarLSRYEQIKKFLLlpepftIENGELTPTLKlKRPV 451
|
|
| PRK09029 |
PRK09029 |
O-succinylbenzoic acid--CoA ligase; Provisional |
63-271 |
1.03e-07 |
|
O-succinylbenzoic acid--CoA ligase; Provisional
Pssm-ID: 236363 [Multi-domain] Cd Length: 458 Bit Score: 54.88 E-value: 1.03e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 63 EKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQREAaLKEGDTAALFLGNEPAYIWIWLglaklgcsmACLNCnirsraf 142
Cdd:PRK09029 14 QVRPQAIALRLNDEVLTWQQLCARIDQLAAGFAQQG-VVEGSGVALRGKNSPETLLAYL---------ALLQC------- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 143 lhsfncsGAKVLIAGADLKGA-VEEVLPNLreNNVTIFYLNDEPITQGVESLKEKIEASSDQPIPKSFRITTTTktaaly 221
Cdd:PRK09029 77 -------GARVLPLNPQLPQPlLEELLPSL--TLDFALVLEGENTFSALTSLHLQLVEGAHAVAWQPQRLATMT------ 141
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|.
gi 556957071 222 iYTSGTTGLPKAAIIN-QRRLWGASFLLSISGVTSEDVVYTPLPLYHSAGL 271
Cdd:PRK09029 142 -LTSGSTGLPKAAVHTaQAHLASAEGVLSLMPFTAQDSWLLSLPLFHVSGQ 191
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
78-577 |
1.16e-07 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 54.78 E-value: 1.16e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 78 YTYLEVDKRSNQVARALQREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKVLIAG 157
Cdd:cd05928 42 WSFRELGSLSRKAANVLSGACGLQRGDRVAVILPRVPEWWLVNVACIRTGLVFIPGTIQLTAKDILYRLQASKAKCIVTS 121
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 158 ADLKGAVEEVL---PNLRennvTIFYLNDEPiTQGVESLKEKIEASSDQPIPksfrITTTTKTAALYIYTSGTTGLPKAA 234
Cdd:cd05928 122 DELAPEVDSVAsecPSLK----TKLLVSEKS-RDGWLNFKELLNEASTEHHC----VETGSQEPMAIYFTSGTTGSPKMA 192
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 235 IINQrrlwgASFLLSIS-------GVTSEDVVYTplplyhsagllLGVNGCIELGTTCVlrrkFS-----ASQFWNDCRE 302
Cdd:cd05928 193 EHSH-----SSLGLGLKvngrywlDLTASDIMWN-----------TSDTGWIKSAWSSL----FEpwiqgACVFVHHLPR 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 303 YNTTAIqyIGEMLRY----LCSTPR------KDNDRDHKVR-----IAVGNGLRADVWKEFLNRFGkIRICEFYAATEGN 367
Cdd:cd05928 253 FDPLVI--LKTLSSYpittFCGAPTvyrmlvQQDLSSYKFPslqhcVTGGEPLNPEVLEKWKAQTG-LDIYEGYGQTETG 329
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 368 IAFINYSG---KIGAVGRANflhkrgVPYELikfntekeePVRDANGHCihVSKGETGLLVAKITTRSPFC---GYAGSK 441
Cdd:cd05928 330 LICANFKGmkiKPGSMGKAS------PPYDV---------QIIDDNGNV--LPPGTEGDIGIRVKPIRPFGlfsGYVDNP 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 442 TQSEKKILRDvfrkgdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVygVTVPEH-E 520
Cdd:cd05928 393 EKTAATIRGD-------FYLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHPAVVESAV--VSSPDPiR 463
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 556957071 521 GRVGMAAIKLKEGKKFDGPN-----IYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKL 577
Cdd:cd05928 464 GEVVKAFVVLAPQFLSHDPEqltkeLQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRNEL 525
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
53-518 |
3.38e-07 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 53.10 E-value: 3.38e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 53 TLLDKFLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLgcsmac 132
Cdd:cd05920 16 PLGDLLARSAARHPDRIAVVDGDRRLTYRELDRRADRLAAGL-RGLGIRPGDRVVVQLPNVAEFVVLFFALLRL------ 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 133 lncnirsraflhsfncsGAKVLIAgadlkgaveevLPNLRENNVTIFYLNDEPIT-------QGVESLKEKIEASSDQPI 205
Cdd:cd05920 89 -----------------GAVPVLA-----------LPSHRRSELSAFCAHAEAVAyivpdrhAGFDHRALARELAESIPE 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 206 PksfrittttktaALYIYTSGTTGLPKaAIINQRRLWGASFLLS--ISGVTSEDVVYTPLPLYHSAGLLL-GVNGCIELG 282
Cdd:cd05920 141 V------------ALFLLSGGTTGTPK-LIPRTHNDYAYNVRASaeVCGLDQDTVYLAVLPAAHNFPLACpGVLGTLLAG 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 283 TTCVLRRKFSASQFWNDCREYNTT--------AIQYIGEMLRylcstpRKDNDRDHKVRIAVGNGLRADVWKEFLNRFGk 354
Cdd:cd05920 208 GRVVLAPDPSPDAAFPLIEREGVTvtalvpalVSLWLDAAAS------RRADLSSLRLLQVGGARLSPALARRVPPVLG- 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 355 IRICEFYAATEGniaFINYSgkigavgranflhKRGVPYELIkFNTE-------KEEPVRDANGHciHVSKGETGLLvak 427
Cdd:cd05920 281 CTLQQVFGMAEG---LLNYT-------------RLDDPDEVI-IHTQgrpmspdDEIRVVDEEGN--PVPPGEEGEL--- 338
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 428 iTTRSP--FCGYAGSKTQSEKkilrdVFRKgDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDF 505
Cdd:cd05920 339 -LTRGPytIRGYYRAPEHNAR-----AFTP-DGFYRTGDLVRRTPDGYLVVEGRIKDQINRGGEKIAAEEVENLLLRHPA 411
|
490
....*....|...
gi 556957071 506 IQEASVygVTVPE 518
Cdd:cd05920 412 VHDAAV--VAMPD 422
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
53-287 |
3.78e-07 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 53.63 E-value: 3.78e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 53 TLLDKFLQQVEKQPHKPFLVF------EKQVYTYLEVDKRSNQVARALQREAALkeGDTAALFLGNEPAYIwiwlgLAKL 126
Cdd:PRK05691 10 TLVQALQRRAAQTPDRLALRFladdpgEGVVLSYRDLDLRARTIAAALQARASF--GDRAVLLFPSGPDYV-----AAFF 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 127 GCSMACL----------NCNIRSRAFLHSFNCSGAKVLIAGADLKGAVEEvLPNLRENNVtifylndePITQGVESLKEK 196
Cdd:PRK05691 83 GCLYAGViavpayppesARRHHQERLLSIIADAEPRLLLTVADLRDSLLQ-MEELAAANA--------PELLCVDTLDPA 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 197 IEASSDQPIPKSFRIttttktaALYIYTSGTTGLPKAAIINQRRLWGASFLLSIS---GVTSEDVVYTPLPLYHSAGLLL 273
Cdd:PRK05691 154 LAEAWQEPALQPDDI-------AFLQYTSGSTALPKGVQVSHGNLVANEQLIRHGfgiDLNPDDVIVSWLPLYHDMGLIG 226
|
250
....*....|....
gi 556957071 274 GVNGCIELGTTCVL 287
Cdd:PRK05691 227 GLLQPIFSGVPCVL 240
|
|
| AFD_CAR-like |
cd17632 |
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ... |
71-531 |
3.92e-07 |
|
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.
Pssm-ID: 341287 [Multi-domain] Cd Length: 588 Bit Score: 53.23 E-value: 3.92e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 71 LVFEKQVYTYLEVDKRSNQVARALQREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSG 150
Cdd:cd17632 61 LLPRFETITYAELWERVGAVAAAHDPEQPVRPGDFVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASAAQLAPILAETE 140
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 151 AKVLIAGAD-LKGAVEEVLPNLRENNVTIFYLNDE----------------PITQGVESLKEKIEASSDQPIPKSFRITT 213
Cdd:cd17632 141 PRLLAVSAEhLDLAVEAVLEGGTPPRLVVFDHRPEvdahraalesarerlaAVGIPVTTLTLIAVRGRDLPPAPLFRPEP 220
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 214 TTKTAALYIYTSGTTGLPKAAIINQR---RLWGASFLLsISGVTSEDVVYTPLPLYHSAGLLLgVNGCIELGTTCVLRRK 290
Cdd:cd17632 221 DDDPLALLIYTSGSTGTPKGAMYTERlvaTFWLKVSSI-QDIRPPASITLNFMPMSHIAGRIS-LYGTLARGGTAYFAAA 298
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 291 FSASQFWNDCREYNTTAIQY---IGEML--RYLCSTPRKDNDRDHKVRIA--VGNGLRADV------------------W 345
Cdd:cd17632 299 SDMSTLFDDLALVRPTELFLvprVCDMLfqRYQAELDRRSVAGADAETLAerVKAELRERVlggrllaavcgsaplsaeM 378
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 346 KEFLNRFGKIRICEFYAATEGNIAFINysgkiGAVGRANFLHKR--GVPyELIKFNTEKEEPvrdanghcihvsKGEtgL 423
Cdd:cd17632 379 KAFMESLLDLDLHDGYGSTEAGAVILD-----GVIVRPPVLDYKlvDVP-ELGYFRTDRPHP------------RGE--L 438
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 424 LVakiTTRSPFCGYAGSktqseKKILRDVFRKgDIFFNSGDLLM-IDHDNFIYFkDRIGDTFRW-KGENVATCEVADLLE 501
Cdd:cd17632 439 LV---KTDTLFPGYYKR-----PEVTAEVFDE-DGFYRTGDVMAeLGPDRLVYV-DRRNNVLKLsQGEFVTVARLEAVFA 508
|
490 500 510
....*....|....*....|....*....|....*....
gi 556957071 502 NVDFIQEASVYG---------VTVPEHEGRVGMAAIKLK 531
Cdd:cd17632 509 ASPLVRQIFVYGnserayllaVVVPTQDALAGEDTARLR 547
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
67-558 |
6.03e-07 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 52.33 E-value: 6.03e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 67 HKPFLVFEKQVyTYLEVDKRSNQVARALQREAaLKEGDTAALFLGNEPAYIWIWLGLAKLGcsMACLNCN--IRSRAFLH 144
Cdd:PRK07059 39 RPAFICMGKAI-TYGELDELSRALAAWLQSRG-LAKGARVAIMMPNVLQYPVAIAAVLRAG--YVVVNVNplYTPRELEH 114
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 145 SFNCSGAKVLIAGADLKGAVEEVLPNLRENNVTIFYLNDEPITQG------VESLKEKIEASSdqpIP--KSF------- 209
Cdd:PRK07059 115 QLKDSGAEAIVVLENFATTVQQVLAKTAVKHVVVASMGDLLGFKGhivnfvVRRVKKMVPAWS---LPghVRFndalaeg 191
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 210 ------RITTTTKTAALYIYTSGTTGLPKAAIINQRRL---------WGASFLLSISGVTSEDVVyTPLPLYHSAGL--- 271
Cdd:PRK07059 192 arqtfkPVKLGPDDVAFLQYTGGTTGVSKGATLLHRNIvanvlqmeaWLQPAFEKKPRPDQLNFV-CALPLYHIFALtvc 270
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 272 -LLGvngcIELGTTCVL---RRKFSAsqFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNGLR-----A 342
Cdd:PRK07059 271 gLLG----MRTGGRNILipnPRDIPG--FIKELKKYQVHIFPAVNTLYNALLNNPDFDKLDFSKLIVANGGGMAvqrpvA 344
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 343 DVWKEFLnrfgKIRICEFYAATEGN-IAFIN------YSGKIGavgranflhkrgVPYElikfNTEKEepVRDANGHciH 415
Cdd:PRK07059 345 ERWLEMT----GCPITEGYGLSETSpVATCNpvdateFSGTIG------------LPLP----STEVS--IRDDDGN--D 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 416 VSKGETGllvaKITTRSP--FCGYAGSKTQSEKKILRDvfrkGdiFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVAT 493
Cdd:PRK07059 401 LPLGEPG----EICIRGPqvMAGYWNRPDETAKVMTAD----G--FFRTGDVGVMDERGYTKIVDRKKDMILVSGFNVYP 470
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 556957071 494 CEVADLLENVDFIQEASVYGvtVP-EHEGRvgmaAIKLKEGKKfdGPN-----IYKHVMEYLPNYARPRFV 558
Cdd:PRK07059 471 NEIEEVVASHPGVLEVAAVG--VPdEHSGE----AVKLFVVKK--DPAlteedVKAFCKERLTNYKRPKFV 533
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
61-368 |
8.42e-07 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 52.47 E-value: 8.42e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 61 QVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQrEAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSR 140
Cdd:PRK12467 521 QARQHPERPALVFGEQVLSYAELNRQANRLAHVLI-AAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPLDPEYPQD 599
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 141 AFLHSFNCSGAKVLIAGadlkgavEEVLPNL-RENNVTIFYLND-EPITQGVESLKEKIEASSDQpipksfrittttktA 218
Cdd:PRK12467 600 RLAYMLDDSGVRLLLTQ-------SHLLAQLpVPAGLRSLCLDEpADLLCGYSGHNPEVALDPDN--------------L 658
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 219 ALYIYTSGTTGLPKAAIINQRRLwgASFLLSIS---GVTSEDVVYtplpLYHSAGLLLGVN---GCIELGTTCVLRRK-- 290
Cdd:PRK12467 659 AYVIYTSGSTGQPKGVAISHGAL--ANYVCVIAerlQLAADDSML----MVSTFAFDLGVTelfGALASGATLHLLPPdc 732
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 291 -FSASQFWNDCREYNTTAIQYIGEMLRYLCSTPRKDNDRDHKVRIAVGNGLRADV---WKEFLNrfgKIRICEFYAATEG 366
Cdd:PRK12467 733 aRDAEAFAALMADQGVTVLKIVPSHLQALLQASRVALPRPQRALVCGGEALQVDLlarVRALGP---GARLINHYGPTET 809
|
..
gi 556957071 367 NI 368
Cdd:PRK12467 810 TV 811
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
66-241 |
1.33e-06 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 51.32 E-value: 1.33e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 66 PHKPFLVFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCsmACLNCNI-----RSR 140
Cdd:cd17656 2 PDAVAVVFENQKLTYRELNERSNQLARFL-REKGVKKDSIVAIMMERSAEMIVGILGILKAGG--AFVPIDPeypeeRRI 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 141 AFLHSfncSGAKVLIAGADLKGAVEEvlpnlrenNVTIFYLNDEPITQGVESLKEKIEASSDqpipksfrittttktaAL 220
Cdd:cd17656 79 YIMLD---SGVRVVLTQRHLKSKLSF--------NKSTILLEDPSISQEDTSNIDYINNSDD----------------LL 131
|
170 180
....*....|....*....|..
gi 556957071 221 Y-IYTSGTTGLPKAAIINQRRL 241
Cdd:cd17656 132 YiIYTSGTTGKPKGVQLEHKNM 153
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
219-294 |
2.95e-06 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 50.13 E-value: 2.95e-06
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 556957071 219 ALYIYTSGTTGLPKAAIINQRRLWG-ASFLLSISGVTSEDVVYTPLPLYHSAGLLLGVNGCIELGTTCVLRRKFSAS 294
Cdd:cd05914 92 ALINYTSGTTGNSKGVMLTYRNIVSnVDGVKEVVLLGKGDKILSILPLHHIYPLTFTLLLPLLNGAHVVFLDKIPSA 168
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
220-565 |
4.08e-06 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 49.30 E-value: 4.08e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 220 LYI-YTSGTTGLPKAAIINQRRLWGASF--------------LLSISGVTSEDVVYTPL-PLYHSAGLLLGVNGCIELGT 283
Cdd:cd05924 6 LYIlYTGGTTGMPKGVMWRQEDIFRMLMggadfgtgeftpseDAHKAAAAAAGTVMFPApPLMHGTGSWTAFGGLLGGQT 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 284 TCVLRRKFSASQFWNDCREYNTTAIQYIGE-MLRYLCSTPRKDNDRDHKVRIAVGNG---LRADVWKEFLNRFGKIRICE 359
Cdd:cd05924 86 VVLPDDRFDPEEVWRTIEKHKVTSMTIVGDaMARPLIDALRDAGPYDLSSLFAISSGgalLSPEVKQGLLELVPNITLVD 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 360 FYAATE-GNIAFINYSGKIGAVGRANFLHKRGVpyeLIKFNTEKEEPVRDANGhcihvskgetgllvaKITTRS--PFcG 436
Cdd:cd05924 166 AFGSSEtGFTGSGHSAGSGPETGPFTRANPDTV---VLDDDGRVVPPGSGGVG---------------WIARRGhiPL-G 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 437 YAGsktqSEKKILRDVFRKGDIFFN-SGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVT 515
Cdd:cd05924 227 YYG----DEAKTAETFPEVDGVRYAvPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRP 302
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|
gi 556957071 516 VPEHEGRVgMAAIKLKEGKKFDGPNIYKHVMEYLPNYARPRFVRIQEAIE 565
Cdd:cd05924 303 DERWGQEV-VAVVQLREGAGVDLEELREHCRTRIARYKLPKQVVFVDEIE 351
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
74-267 |
6.32e-06 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 49.01 E-value: 6.32e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 74 EKQVYTYLEVDKRSNQVARALQREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKV 153
Cdd:PRK05620 35 EQEQTTFAAIGARAAALAHALHDELGITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMNDQIVHIINHAEDEV 114
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 154 LIAGADLKGAVEEVLPNLRENNVTIFyLNDEPITQGVESLKEKIEA----------SSDQPIPKsfritTTTKTAALYIY 223
Cdd:PRK05620 115 IVADPRLAEQLGEILKECPCVRAVVF-IGPSDADSAAAHMPEGIKVysyealldgrSTVYDWPE-----LDETTAAAICY 188
|
170 180 190 200
....*....|....*....|....*....|....*....|....*..
gi 556957071 224 TSGTTGLPKAAIINQRRLWGASFLLSIS---GVTSEDVVYTPLPLYH 267
Cdd:PRK05620 189 STGTTGAPKGVVYSHRSLYLQSLSLRTTdslAVTHGESFLCCVPIYH 235
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
66-562 |
8.94e-06 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 48.52 E-value: 8.94e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 66 PHKPFLVFEKQVYTYLEVDKRSNQVARALQREAALKEgDTAALFLGNEPAYIWIWLGLAKLGCsmACLNCNIRSRA--FL 143
Cdd:cd17649 1 PDAVALVFGDQSLSYAELDARANRLAHRLRALGVGPE-VRVGIALERSLEMVVALLAILKAGG--AYVPLDPEYPAerLR 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 144 HSFNCSGAKVLiagadlkgaveevlpnlrennvtifylndepitqgveslkekieassdqpipksfrITTTTKTAALYIY 223
Cdd:cd17649 78 YMLEDSGAGLL--------------------------------------------------------LTHHPRQLAYVIY 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 224 TSGTTGLPKAAIINQRRLwgASFLLSIS---GVTSEDVVYTPLPLYHSAGLLLGVNGCIElGTTCVLRrkfsASQFWNDC 300
Cdd:cd17649 102 TSGSTGTPKGVAVSHGPL--AAHCQATAeryGLTPGDRELQFASFNFDGAHEQLLPPLIC-GACVVLR----PDELWASA 174
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 301 REYnTTAIQ------------YIGEMLRYLCSTPRkdnDRDHKVRIAVGNGLRADVwkEFLNRFGKI--RICEFYAATEG 366
Cdd:cd17649 175 DEL-AEMVRelgvtvldlppaYLQQLAEEADRTGD---GRPPSLRLYIFGGEALSP--ELLRRWLKApvRLFNAYGPTEA 248
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 367 NIAFINYSGKIGAvgranflhKRGVPYELIKfnteKEEPVRDA---NGHCIHVSKGETGLL------VAKittrspfcGY 437
Cdd:cd17649 249 TVTPLVWKCEAGA--------ARAGASMPIG----RPLGGRSAyilDADLNPVPVGVTGELyiggegLAR--------GY 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 438 AGSKTQSEKKILRDVF-RKGDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTV 516
Cdd:cd17649 309 LGRPELTAERFVPDPFgAPGSRLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIELGEIEAALLEHPGVREAAVVALDG 388
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 556957071 517 PEHEGRVGMAAIKLKEGKKFDGPNIYKHVMEYLPNYARP-RFVRIQE 562
Cdd:cd17649 389 AGGKQLVAYVVLRAAAAQPELRAQLRTALRASLPDYMVPaHLVFLAR 435
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
50-262 |
9.66e-06 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 48.89 E-value: 9.66e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 50 PVFTLLDKFLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLgnePAYIWIWLGL-AKLGC 128
Cdd:PRK10252 456 PETTLSALVAQQAAKTPDAPALADARYQFSYREMREQVVALANLL-RERGVKPGDSVAVAL---PRSVFLTLALhAIVEA 531
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 129 SMACLNCNI-----RSRAFLHSfncSGAKVLIAGADLkgaveevLPNLRENNVTIFYLNDEPITqgveslkekieASSDQ 203
Cdd:PRK10252 532 GAAWLPLDTgypddRLKMMLED---ARPSLLITTADQ-------LPRFADVPDLTSLCYNAPLA-----------PQGAA 590
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 556957071 204 PIPKSfrittTTKTAALYIYTSGTTGLPK------AAIINqRRLWgasfLLSISGVTSEDVVY--TP 262
Cdd:PRK10252 591 PLQLS-----QPHHTAYIIFTSGSTGRPKgvmvgqTAIVN-RLLW----MQNHYPLTADDVVLqkTP 647
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
58-257 |
1.86e-05 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 47.55 E-value: 1.86e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 58 FLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNI 137
Cdd:cd17645 4 FEEQVERTPDHVAVVDRGQSLTYKQLNEKANQLARHL-RGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDPDY 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 138 RSRAFLHSFNCSGAKVLIAGADlkgaveevlpNLrennvtifylndepitqgveslkekieassdqpipksfrittttkt 217
Cdd:cd17645 83 PGERIAYMLADSSAKILLTNPD----------DL---------------------------------------------- 106
|
170 180 190 200
....*....|....*....|....*....|....*....|....*
gi 556957071 218 aALYIYTSGTTGLPKAAIINQRRL-----WGASFLlsisGVTSED 257
Cdd:cd17645 107 -AYVIYTSGSTGLPKGVMIEHHNLvnlceWHRPYF----GVTPAD 146
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
151-382 |
2.28e-05 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 47.65 E-value: 2.28e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 151 AKVLIAGADLKGAVEEVlpnlrENNVTIFYLNDepITQGVeSLKEKIEASsdqpIPKSFRITTTTK----TAALYIYTSG 226
Cdd:PRK06814 736 SRAFIEKARLGPLIEAL-----EFGIRIIYLED--VRAQI-GLADKIKGL----LAGRFPLVYFCNrdpdDPAVILFTSG 803
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 227 TTGLPKAAIINQRRLWGASF-LLSISGVTSEDVVYTPLPLYHSAGLLLG-----VNG--------------CIEL----G 282
Cdd:PRK06814 804 SEGTPKGVVLSHRNLLANRAqVAARIDFSPEDKVFNALPVFHSFGLTGGlvlplLSGvkvflypsplhyriIPELiydtN 883
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 283 TTCVlrrkFSASQFWNDcreYNTTAIQYIGEMLRYLcstprkdndrdhkvrIAVGNGLRADVWKEFLNRFGkIRICEFYA 362
Cdd:PRK06814 884 ATIL----FGTDTFLNG---YARYAHPYDFRSLRYV---------------FAGAEKVKEETRQTWMEKFG-IRILEGYG 940
|
250 260
....*....|....*....|...
gi 556957071 363 ATE-GNIAFIN--YSGKIGAVGR 382
Cdd:PRK06814 941 VTEtAPVIALNtpMHNKAGTVGR 963
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
72-267 |
3.23e-05 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 46.89 E-value: 3.23e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 72 VFEKQVY-TYLEVDKRSNQVARALqREAALKEGDTAALFLGNEpayiWIWLGLAkLGC---SM--ACLNCNIRSRAFLHS 145
Cdd:PTZ00216 115 HFNETRYiTYAELWERIVNFGRGL-AELGLTKGSNVAIYEETR----WEWLASI-YGIwsqSMvaATVYANLGEDALAYA 188
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 146 FNCSGAKVLIAGADlkgAVEEVLPNLREN---NVTIFYLNDEPitQGVES-----------LKEKIEASSDQPIpksfRI 211
Cdd:PTZ00216 189 LRETECKAIVCNGK---NVPNLLRLMKSGgmpNTTIIYLDSLP--ASVDTegcrlvawtdvVAKGHSAGSHHPL----NI 259
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 556957071 212 TTTTKTAALYIYTSGTTGLPKAAI------------INQRrlwgasfLLSISGVTSEDVVYTP-LPLYH 267
Cdd:PTZ00216 260 PENNDDLALIMYTSGTTGDPKGVMhthgsltagilaLEDR-------LNDLIGPPEEDETYCSyLPLAH 321
|
|
| PLN02387 |
PLN02387 |
long-chain-fatty-acid-CoA ligase family protein |
49-267 |
4.98e-05 |
|
long-chain-fatty-acid-CoA ligase family protein
Pssm-ID: 215217 [Multi-domain] Cd Length: 696 Bit Score: 46.26 E-value: 4.98e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 49 EPVFTLLDKFLQQVEKQPHKPFL-----------------VFEK------QVYTYLEVDKRSNQVARALqreAAL--KEG 103
Cdd:PLN02387 55 EGATTLAALFEQSCKKYSDKRLLgtrklisrefetssdgrKFEKlhlgeyEWITYGQVFERVCNFASGL---VALghNKE 131
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 104 DTAALFLGNEPAyiwiWLgLAKLGC-----SMACLNCNIRSRAFLHSFNCSGAKVLIAGA-DLKG--AVEEVLPNLREnn 175
Cdd:PLN02387 132 ERVAIFADTRAE----WL-IALQGCfrqniTVVTIYASLGEEALCHSLNETEVTTVICDSkQLKKliDISSQLETVKR-- 204
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 176 vtIFYLNDEPITQG----------VESLKEKIEASSDQPIPKSFRITTTTktaALYIYTSGTTGLPKAAIINQRRLWG-- 243
Cdd:PLN02387 205 --VIYMDDEGVDSDsslsgssnwtVSSFSEVEKLGKENPVDPDLPSPNDI---AVIMYTSGSTGLPKGVMMTHGNIVAtv 279
|
250 260
....*....|....*....|....
gi 556957071 244 ASFLLSISGVTSEDVVYTPLPLYH 267
Cdd:PLN02387 280 AGVMTVVPKLGKNDVYLAYLPLAH 303
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
79-580 |
1.28e-04 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 45.13 E-value: 1.28e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 79 TYLEVDKRSNQVARALQREAaLKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIRSRAFLHSFNCSGAKVLIAGA 158
Cdd:PRK06018 41 TYAQIHDRALKVSQALDRDG-IKLGDRVATIAWNTWRHLEAWYGIMGIGAICHTVNPRLFPEQIAWIINHAEDRVVITDL 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 159 DLKGAVEEVLPNLRENNVTIFYLNDEPITQ----GVESLKEKIEASSDQPIPKSFrittTTKTAALYIYTSGTTGLPKAA 234
Cdd:PRK06018 120 TFVPILEKIADKLPSVERYVVLTDAAHMPQttlkNAVAYEEWIAEADGDFAWKTF----DENTAAGMCYTSGTTGDPKGV 195
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 235 IINQRrlwgASFLLSIS-------GVTSEDVVYTPLPLYHSAGLLLGVNgCIELGTTCVL--RRKFSAS--QFWNDCREY 303
Cdd:PRK06018 196 LYSHR----SNVLHALManngdalGTSAADTMLPVVPLFHANSWGIAFS-APSMGTKLVMpgAKLDGASvyELLDTEKVT 270
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 304 NTTAIQYIGEML-RYLCSTPRKDNDRDhKVRIAvGNGLRADVWKEFLNRFGKIRicEFYAATE----GNIA-----FINY 373
Cdd:PRK06018 271 FTAGVPTVWLMLlQYMEKEGLKLPHLK-MVVCG-GSAMPRSMIKAFEDMGVEVR--HAWGMTEmsplGTLAalkppFSKL 346
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 374 SGKigavGRANFLHKRGVPyeliKFNTEKEePVRDANGHCIHVSKGETGLLVakittRSPfcGYAGSKTQSEKKILRDvf 453
Cdd:PRK06018 347 PGD----ARLDVLQKQGYP----PFGVEMK-ITDDAGKELPWDGKTFGRLKV-----RGP--AVAAAYYRVDGEILDD-- 408
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 454 rkgDIFFNSGDLLMIDHDNFIYFKDRIGDTFRWKGENVATCEVADLLENVDFIQEASVYGVTVPEHEGRvGMAAIKLKEG 533
Cdd:PRK06018 409 ---DGFFDTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHPKWDER-PLLIVQLKPG 484
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 556957071 534 KKFDGPNIYKHVMEYLPNYARPRFVRIQEAIEITGTFKQRKVKLVEE 580
Cdd:PRK06018 485 ETATREEILKYMDGKIAKWWMPDDVAFVDAIPHTATGKILKTALREQ 531
|
|
| PRK03584 |
PRK03584 |
acetoacetate--CoA ligase; |
64-233 |
5.04e-04 |
|
acetoacetate--CoA ligase;
Pssm-ID: 235134 [Multi-domain] Cd Length: 655 Bit Score: 43.25 E-value: 5.04e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 64 KQPHKPFLVF-----EKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMACLNCNIR 138
Cdd:PRK03584 96 RRDDRPAIIFrgedgPRRELSWAELRRQVAALAAAL-RALGVGPGDRVAAYLPNIPETVVAMLATASLGAIWSSCSPDFG 174
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 139 SRAFLHSFNCSGAKVLIAGA---------DLKGAVEEV---LPNLrENNVTIFYLNDEPITQGVESLKEKIEASSDQPiP 206
Cdd:PRK03584 175 VQGVLDRFGQIEPKVLIAVDgyryggkafDRRAKVAELraaLPSL-EHVVVVPYLGPAAAAAALPGALLWEDFLAPAE-A 252
|
170 180
....*....|....*....|....*...
gi 556957071 207 KSFRITTTTKTAALYI-YTSGTTGLPKA 233
Cdd:PRK03584 253 AELEFEPVPFDHPLWIlYSSGTTGLPKC 280
|
|
| PRK05850 |
PRK05850 |
acyl-CoA synthetase; Validated |
202-287 |
6.87e-04 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235624 [Multi-domain] Cd Length: 578 Bit Score: 42.62 E-value: 6.87e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 202 DQPIPKSFRITTTTKTAALYiYTSGTTGLPKAAIINQRRLWG------ASFLLSISGVTSED-VVYTPLPLYHSAGLLLG 274
Cdd:PRK05850 147 DSPRGSDARPRDLPSTAYLQ-YTSGSTRTPAGVMVSHRNVIAnfeqlmSDYFGDTGGVPPPDtTVVSWLPFYHDMGLVLG 225
|
90
....*....|...
gi 556957071 275 VNGCIELGTTCVL 287
Cdd:PRK05850 226 VCAPILGGCPAVL 238
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
53-236 |
7.66e-04 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 42.85 E-value: 7.66e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 53 TLLDKFLQQVEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGNEPAYIWIWLGLAKLGCSMAC 132
Cdd:PRK05691 2189 TLHGLFAAQAARTPQAPALTFAGQTLSYAELDARANRLARAL-RERGVGPQVRVGLALERSLEMVVGLLAILKAGGAYVP 2267
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 133 LNCNIRSRAFLHSFNCSGAKVLIAGADLKGAVEEvLPnlreNNVTIFYLNDEPITqgveslkekIEASSDQPIPksfRIT 212
Cdd:PRK05691 2268 LDPEYPLERLHYMIEDSGIGLLLSDRALFEALGE-LP----AGVARWCLEDDAAA---------LAAYSDAPLP---FLS 2330
|
170 180
....*....|....*....|....
gi 556957071 213 TTTKTAALyIYTSGTTGLPKAAII 236
Cdd:PRK05691 2331 LPQHQAYL-IYTSGSTGKPKGVVV 2353
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
34-127 |
9.11e-04 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 42.28 E-value: 9.11e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 34 RTIPFN---------YKMKKFEEKEPVFTLLDKflqqvEKQPHKPFLVFEKQVYTYLEVDKRSNQVARALQReAALKEGD 104
Cdd:PRK10946 1 MSIPFTrwpeefarrYREKGYWQDLPLTDILTR-----HAASDAIAVICGERQFSYRELNQASDNLACSLRR-QGIKPGD 74
|
90 100
....*....|....*....|...
gi 556957071 105 TAALFLGNEPAYIWIWLGLAKLG 127
Cdd:PRK10946 75 TALVQLGNVAEFYITFFALLKLG 97
|
|
| PLN02736 |
PLN02736 |
long-chain acyl-CoA synthetase |
42-267 |
1.20e-03 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 178337 [Multi-domain] Cd Length: 651 Bit Score: 42.01 E-value: 1.20e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 42 MKKFEEKEPVFTLLDKFLQQVEKQPHKPFL---------VFEKQVYTYLEVDKRSNQVARALqREAALKEGDTAALFLGN 112
Cdd:PLN02736 34 VSRFPDHPEIGTLHDNFVYAVETFRDYKYLgtrirvdgtVGEYKWMTYGEAGTARTAIGSGL-VQHGIPKGACVGLYFIN 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 113 EP----------AYIWI------WLG------------LAKLGCSMACLNCNIrsrAFLHSFNCSGAKVLIAGADlkgav 164
Cdd:PLN02736 113 RPewlivdhacsAYSYVsvplydTLGpdavkfivnhaeVAAIFCVPQTLNTLL---SCLSEIPSVRLIVVVGGAD----- 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 165 eEVLPNLrennvtifylndePITQGVESLK-EKIEA---SSDQPI--PKSFRITTTTktaalyiYTSGTTGLPKAAIINQ 238
Cdd:PLN02736 185 -EPLPSL-------------PSGTGVEIVTySKLLAqgrSSPQPFrpPKPEDVATIC-------YTSGTTGTPKGVVLTH 243
|
250 260 270
....*....|....*....|....*....|...
gi 556957071 239 RRL----WGASFLLSISgvtSEDVVYTPLPLYH 267
Cdd:PLN02736 244 GNLianvAGSSLSTKFY---PSDVHISYLPLAH 273
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
197-272 |
2.42e-03 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 40.73 E-value: 2.42e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 197 IEASSDQPIPKSFRITTTTkTAALYIYTSGTTGLPKAAIINQR----RLWGASfllSISGVTSEDVVYTPLPLYHSAGLL 272
Cdd:cd05906 149 IEELLDTAADHDLPQSRPD-DLALLMLTSGSTGFPKAVPLTHRnilaRSAGKI---QHNGLTPQDVFLNWVPLDHVGGLV 224
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
78-267 |
3.22e-03 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 40.28 E-value: 3.22e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 78 YTYLEVDKRSNQVARALQREAALKEGDTA-ALFLGNEPAYIwiwlgLAKLGC---SMAC--LNCNIRSRAFLHSFNCSGA 151
Cdd:cd05927 6 ISYKEVAERADNIGSALRSLGGKPAPASFvGIYSINRPEWI-----ISELACyaySLVTvpLYDTLGPEAIEYILNHAEI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556957071 152 KVLIAGADLKgaveevlpnlrennvtiFYLNDEPITQGveslKEKIEassdQPIPKsfrittTTKTAALYIYTSGTTGLP 231
Cdd:cd05927 81 SIVFCDAGVK-----------------VYSLEEFEKLG----KKNKV----PPPPP------KPEDLATICYTSGTTGNP 129
|
170 180 190 200
....*....|....*....|....*....|....*....|.
gi 556957071 232 KAAIINQRRLWGAS-----FLLSISGVTSEDVVYTPLPLYH 267
Cdd:cd05927 130 KGVMLTHGNIVSNVagvfkILEILNKINPTDVYISYLPLAH 170
|
|
| |
